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J Phys Chem B
2009 Sep 10;11336:12358-63. doi: 10.1021/jp904154w.
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Molecular interactions between magainin 2 and model membranes in situ.
Nguyen KT
,
Le Clair SV
,
Ye S
,
Chen Z
.
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In this paper, we investigated the molecular interactions of magainin 2 with model cell membranes using sum frequency generation (SFG) vibrational spectroscopy and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). Symmetric 1-palmitoyl-2-oleoyl-sn-glycero-3-[Phospho-rac-(1-glycerol)] (POPG) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayers, which model the bacterial and mammalian cell membranes, respectively, were used in the studies. It was observed by SFG that magainin 2 orients relatively parallel to the POPG lipid bilayer surface at low solution concentrations, around 200 nM. When increasing the magainin 2 concentration to 800 nM, both SFG and ATR-FTIR results indicate that magainin 2 molecules insert into the POPG bilayer and adopt a transmembrane orientation with an angle of about 20 degrees from the POPG bilayer normal. For the POPC bilayer, even at a much higher peptide concentration of 2.0 microM, no ATR-FTIR signal was detected. For this concentration on POPC, SFG studies indicated that magainin 2 molecules adopt an orientation nearly parallel to the bilayer surface, with an orientation angle of about 75 degrees from the surface normal. This shows that SFG has a much better detection limit than ATR-FTIR and can therefore be applied to study interfacial molecules with a much lower surface coverage. This magainin 2 orientation study and further investigation of the lipid bilayer SFG signals support the proposed toroidal pore model for the antimicrobial activity of magainin 2.
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19728722
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Axelsen,
The infrared dichroism of transmembrane helical polypeptides.
1995, Pubmed
Axelsen,
The infrared dichroism of transmembrane helical polypeptides.
1995,
Pubmed
Axelsen,
Orientational order determination by internal reflection infrared spectroscopy.
1996,
Pubmed
Baldelli,
Surface structure at the ionic liquid-electrified metal interface.
2008,
Pubmed
Bechinger,
Orientations of amphipathic helical peptides in membrane bilayers determined by solid-state NMR spectroscopy.
1991,
Pubmed
,
Xenbase
Bechinger,
Structure and orientation of the antibiotic peptide magainin in membranes by solid-state nuclear magnetic resonance spectroscopy.
1993,
Pubmed
,
Xenbase
Brasseur,
Differentiation of lipid-associating helices by use of three-dimensional molecular hydrophobicity potential calculations.
1991,
Pubmed
Chen,
Evidence for membrane thinning effect as the mechanism for peptide-induced pore formation.
2003,
Pubmed
Chen,
SFG studies on interactions between antimicrobial peptides and supported lipid bilayers.
2006,
Pubmed
Chen,
Real-time structural investigation of a lipid bilayer during its interaction with melittin using sum frequency generation vibrational spectroscopy.
2007,
Pubmed
Chen,
Observing a molecular knife at work.
2006,
Pubmed
Chen,
In situ investigation of heterotrimeric G protein betagamma subunit binding and orientation on membrane bilayers.
2007,
Pubmed
Chen,
Multiple orientation of melittin inside a single lipid bilayer determined by combined vibrational spectroscopic studies.
2007,
Pubmed
Gregory,
Magainin 2 revisited: a test of the quantitative model for the all-or-none permeabilization of phospholipid vesicles.
2009,
Pubmed
,
Xenbase
Hallock,
MSI-78, an analogue of the magainin antimicrobial peptides, disrupts lipid bilayer structure via positive curvature strain.
2003,
Pubmed
,
Xenbase
Hirsh,
Secondary structure and location of a magainin analogue in synthetic phospholipid bilayers.
1996,
Pubmed
,
Xenbase
Illya,
Coarse-grained simulation studies of peptide-induced pore formation.
2008,
Pubmed
Imura,
Action mechanism of PEGylated magainin 2 analogue peptide.
2007,
Pubmed
,
Xenbase
Imura,
Magainin 2 in action: distinct modes of membrane permeabilization in living bacterial and mammalian cells.
2008,
Pubmed
,
Xenbase
Jackson,
Conformation of magainin-2 and related peptides in aqueous solution and membrane environments probed by Fourier transform infrared spectroscopy.
1992,
Pubmed
,
Xenbase
Kim,
Elucidating changes in interfacial water structure upon protein adsorption.
2001,
Pubmed
Kim,
Molecular packing of lysozyme, fibrinogen, and bovine serum albumin on hydrophilic and hydrophobic surfaces studied by infrared-visible sum frequency generation and fluorescence microscopy.
2003,
Pubmed
Lad,
Antimicrobial peptide-lipid binding interactions and binding selectivity.
2007,
Pubmed
Li,
Surface structure relaxation of poly(methyl methacrylate).
2008,
Pubmed
Ludtke,
Membrane pores induced by magainin.
1996,
Pubmed
,
Xenbase
Ludtke,
Membrane thinning caused by magainin 2.
1995,
Pubmed
,
Xenbase
Maloy,
Structure-activity studies on magainins and other host defense peptides.
1995,
Pubmed
Matsuzaki,
Physicochemical determinants for the interactions of magainins 1 and 2 with acidic lipid bilayers.
1991,
Pubmed
,
Xenbase
Matsuzaki,
An antimicrobial peptide, magainin 2, induced rapid flip-flop of phospholipids coupled with pore formation and peptide translocation.
1996,
Pubmed
,
Xenbase
Matsuzaki,
Orientational and aggregational states of magainin 2 in phospholipid bilayers.
1994,
Pubmed
,
Xenbase
Matsuzaki,
Magainins as paradigm for the mode of action of pore forming polypeptides.
1998,
Pubmed
,
Xenbase
Matsuzaki,
Magainin 1-induced leakage of entrapped calcein out of negatively-charged lipid vesicles.
1989,
Pubmed
,
Xenbase
Mecke,
Membrane thinning due to antimicrobial peptide binding: an atomic force microscopy study of MSI-78 in lipid bilayers.
2005,
Pubmed
Murzyn,
Construction of a toroidal model for the magainin pore.
2003,
Pubmed
,
Xenbase
Tamm,
Infrared spectroscopy of proteins and peptides in lipid bilayers.
1997,
Pubmed
Tamm,
Supported phospholipid bilayers.
1985,
Pubmed
Wang,
Measuring polymer surface ordering differences in air and water by sum frequency generation vibrational spectroscopy.
2002,
Pubmed
Wang,
Quantifying the ordering of adsorbed proteins in situ.
2008,
Pubmed
Wieprecht,
Conformational and functional study of magainin 2 in model membrane environments using the new approach of systematic double-D-amino acid replacement.
1996,
Pubmed
,
Xenbase
Wieprecht,
Binding of antibacterial magainin peptides to electrically neutral membranes: thermodynamics and structure.
1999,
Pubmed
,
Xenbase
Williams,
Raman spectroscopy of synthetic antimicrobial frog peptides magainin 2a and PGLa.
1990,
Pubmed
,
Xenbase
Yatawara,
Carbohydrate surface attachment characterized by sum frequency generation spectroscopy.
2009,
Pubmed
Ye,
Kinetics of ultraviolet and plasma surface modification of poly(dimethylsiloxane) probed by sum frequency vibrational spectroscopy.
2006,
Pubmed
Ye,
In situ molecular level studies on membrane related peptides and proteins in real time using sum frequency generation vibrational spectroscopy.
2009,
Pubmed
Zasloff,
Antimicrobial activity of synthetic magainin peptides and several analogues.
1988,
Pubmed
,
Xenbase
