Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Invert Neurosci
2009 Nov 01;92:77-84. doi: 10.1007/s10158-009-0089-7.
Show Gene links
Show Anatomy links
Alternative splicing of the Anopheles gambiae nicotinic acetylcholine receptor, Agamalphabeta9, generates both alpha and beta subunits.
Jones AK
,
Buckingham SD
,
Brown LA
,
Sattelle DB
.
???displayArticle.abstract???
Nicotinic acetylcholine receptors (nAChRs) are the members of the cys-loop ligand-gated ion channel superfamily and are formed by five subunits arranged around a central ion channel. Each subunit is encoded by a separate gene and is classified as either alpha or non-alpha depending on the presence or absence, respectively, of two adjacent cysteine residues which are important for acetylcholine binding. Here, we report for the first time a single nAChR gene encoding both alpha and non-alpha subunits. Specifically, alternative splicing of the Anopheles gambiae nAChR subunit, previously called Agamalpha9 and renamed here Agamalphabeta9, generates two variants, one possessing the two cysteines (denoted Agamalphabeta9(alpha)) and the other lacking the cysteine doublet (Agamalphabeta9(beta)). Attempts to heterologously express functional nAChRs consisting of the Agamalphabeta9 splice variants in Xenopus laevis oocytes were unsuccessful. Our findings further characterise a potential target to control the malaria mosquito as well as provide insights into the diversification of nAChRs.
Altschul,
Basic local alignment search tool.
1990, Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Ballivet,
Nicotinic acetylcholine receptors in the nematode Caenorhabditis elegans.
1996,
Pubmed
,
Xenbase
Bendtsen,
Improved prediction of signal peptides: SignalP 3.0.
2004,
Pubmed
Bentley,
ShAR1alpha and ShAR1beta: novel putative nicotinic acetylcholine receptor subunits from the platyhelminth blood fluke Schistosoma.
2004,
Pubmed
,
Xenbase
Bertrand,
Physiological properties of neuronal nicotinic receptors reconstituted from the vertebrate beta 2 subunit and Drosophila alpha subunits.
1994,
Pubmed
,
Xenbase
Borges,
Agrin-induced phosphorylation of the acetylcholine receptor regulates cytoskeletal anchoring and clustering.
2001,
Pubmed
Boulin,
Eight genes are required for functional reconstitution of the Caenorhabditis elegans levamisole-sensitive acetylcholine receptor.
2008,
Pubmed
,
Xenbase
Buckingham,
Oocytes as an expression system for studying receptor/channel targets of drugs and pesticides.
2006,
Pubmed
,
Xenbase
Castillo,
Improved gating of a chimeric alpha7-5HT3A receptor upon mutations at the M2-M3 extracellular loop.
2006,
Pubmed
,
Xenbase
Corringer,
Nicotinic receptors at the amino acid level.
2000,
Pubmed
Couturier,
A neuronal nicotinic acetylcholine receptor subunit (alpha 7) is developmentally regulated and forms a homo-oligomeric channel blocked by alpha-BTX.
1990,
Pubmed
,
Xenbase
Felsenstein,
CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP.
1985,
Pubmed
Gao,
The nicotinic acetylcholine receptor subunits Mdalpha5 and Mdbeta3 on autosome 1 of Musca domestica are not involved in spinosad resistance.
2007,
Pubmed
Grauso,
Novel putative nicotinic acetylcholine receptor subunit genes, Dalpha5, Dalpha6 and Dalpha7, in Drosophila melanogaster identify a new and highly conserved target of adenosine deaminase acting on RNA-mediated A-to-I pre-mRNA editing.
2002,
Pubmed
Green,
The role of the cystine loop in acetylcholine receptor assembly.
1997,
Pubmed
Hemingway,
The molecular basis of insecticide resistance in mosquitoes.
2004,
Pubmed
Hopfield,
Functional modulation of the nicotinic acetylcholine receptor by tyrosine phosphorylation.
1988,
Pubmed
Hulo,
The PROSITE database.
2006,
Pubmed
Jensen,
Charge selectivity of the Cys-loop family of ligand-gated ion channels.
2005,
Pubmed
Jeschke,
Neonicotinoids-from zero to hero in insecticide chemistry.
2008,
Pubmed
Jin,
RNA editing and alternative splicing of the insect nAChR subunit alpha6 transcript: evolutionary conservation, divergence and regulation.
2007,
Pubmed
Jones,
The nicotinic acetylcholine receptor gene family of the nematode Caenorhabditis elegans: an update on nomenclature.
2007,
Pubmed
Jones,
The nicotinic acetylcholine receptor gene family of the malaria mosquito, Anopheles gambiae.
2005,
Pubmed
Jones,
Insect nicotinic acetylcholine receptor gene families: from genetic model organism to vector, pest and beneficial species.
2007,
Pubmed
Jones,
The cys-loop ligand-gated ion channel gene superfamily of the red flour beetle, Tribolium castaneum.
2007,
Pubmed
Jones,
The nicotinic acetylcholine receptor gene family of the honey bee, Apis mellifera.
2006,
Pubmed
Kao,
Acetylcholine receptor binding site contains a disulfide cross-link between adjacent half-cystinyl residues.
1986,
Pubmed
Kerah-Hinzoumbé,
Insecticide resistance in Anopheles gambiae from south-western Chad, Central Africa.
2008,
Pubmed
Lansdell,
Dbeta3, an atypical nicotinic acetylcholine receptor subunit from Drosophila : molecular cloning, heterologous expression and coassembly.
2002,
Pubmed
Lansdell,
Cloning and heterologous expression of Dalpha4, a Drosophila neuronal nicotinic acetylcholine receptor subunit: identification of an alternative exon influencing the efficiency of subunit assembly.
2000,
Pubmed
Lansdell,
Host-cell specific effects of the nicotinic acetylcholine receptor chaperone RIC-3 revealed by a comparison of human and Drosophila RIC-3 homologues.
2008,
Pubmed
Le Novère,
Molecular evolution of the nicotinic acetylcholine receptor: an example of multigene family in excitable cells.
1995,
Pubmed
Littleton,
Ion channels and synaptic organization: analysis of the Drosophila genome.
2000,
Pubmed
Matsuda,
Neonicotinoids: insecticides acting on insect nicotinic acetylcholine receptors.
2001,
Pubmed
Millar,
RIC-3: a nicotinic acetylcholine receptor chaperone.
2008,
Pubmed
Millar,
Nicotinic acetylcholine receptors: targets for commercially important insecticides.
2007,
Pubmed
Millar,
Diversity of vertebrate nicotinic acetylcholine receptors.
2009,
Pubmed
Müller,
Decline of Anopheles sergentii and Aedes caspius populations following presentation of attractive toxic (spinosad) sugar bait stations in an oasis.
2008,
Pubmed
Page,
TreeView: an application to display phylogenetic trees on personal computers.
1996,
Pubmed
Pérez,
Spinosad, a naturally derived insecticide, for control of Aedes aegypti (Diptera: Culicidae): efficacy, persistence, and elicited oviposition response.
2007,
Pubmed
Saitou,
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
1987,
Pubmed
Sattelle,
Edit, cut and paste in the nicotinic acetylcholine receptor gene family of Drosophila melanogaster.
2005,
Pubmed
Shao,
The nicotinic acetylcholine receptor gene family of the silkworm, Bombyx mori.
2007,
Pubmed
Sine,
Recent advances in Cys-loop receptor structure and function.
2006,
Pubmed
Szarecka,
Dynamics of heteropentameric nicotinic acetylcholine receptor: implications of the gating mechanism.
2007,
Pubmed
Tasneem,
Identification of the prokaryotic ligand-gated ion channels and their implications for the mechanisms and origins of animal Cys-loop ion channels.
2005,
Pubmed
Thany,
Exploring the pharmacological properties of insect nicotinic acetylcholine receptors.
2007,
Pubmed
Thompson,
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
1997,
Pubmed
Thompson,
The 5-HT3 receptor as a therapeutic target.
2007,
Pubmed
Treinin,
RIC-3 and nicotinic acetylcholine receptors: biogenesis, properties, and diversity.
2008,
Pubmed
Unwin,
Refined structure of the nicotinic acetylcholine receptor at 4A resolution.
2005,
Pubmed
Yassin,
Characterization of the deg-3/des-2 receptor: a nicotinic acetylcholine receptor that mutates to cause neuronal degeneration.
2001,
Pubmed
,
Xenbase