Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Virol
2004 Mar 01;785:2615-9. doi: 10.1128/jvi.78.5.2615-2619.2004.
Show Gene links
Show Anatomy links
Bovine papillomavirus replicative helicase E1 is a target of the ubiquitin ligase APC.
Mechali F, Hsu CY, Castro A, Lorca T, Bonne-Andrea C.
???displayArticle.abstract???
The papillomavirus E1 replicative helicase is essential for replication and maintenance of extrachromosomal viral genomes in infected cells. We previously found that the bovine papillomavirus E1 protein is a substrate of the ubiquitin-dependent proteolytic pathway. Here we show that E1 is targeted for degradation by the anaphase-promoting complex (APC). Inhibition of APC activity by the specific inhibitor Emi1 or point mutations in the D-box and KEN-box motifs of E1 stabilize the protein and increase viral DNA replication in both a cell-free system and in living cells. These findings involve APC as the ubiquitin ligase that controls E1 levels to maintain a constant low copy number of the viral genome during latent infection.
Bermejo,
Regulation of CDC6, geminin, and CDT1 in human cells that undergo polyploidization.
2002, Pubmed
Bermejo,
Regulation of CDC6, geminin, and CDT1 in human cells that undergo polyploidization.
2002,
Pubmed Bonne-Andrea,
Bovine papillomavirus E1 protein can, by itself, efficiently drive multiple rounds of DNA synthesis in vitro.
1995,
Pubmed Castro,
APC/Fizzy-Related targets Aurora-A kinase for proteolysis.
2002,
Pubmed
,
Xenbase Cueille,
Functional interaction between the bovine papillomavirus virus type 1 replicative helicase E1 and cyclin E-Cdk2.
1998,
Pubmed
,
Xenbase Furstenthal,
Cyclin E uses Cdc6 as a chromatin-associated receptor required for DNA replication.
2001,
Pubmed
,
Xenbase Harper,
The anaphase-promoting complex: it's not just for mitosis any more.
2002,
Pubmed Holt,
DNA binding specificity of the bovine papillomavirus E1 protein is determined by sequences contained within an 18-base-pair inverted repeat element at the origin of replication.
1994,
Pubmed Hsu,
E2F-dependent accumulation of hEmi1 regulates S phase entry by inhibiting APC(Cdh1).
2002,
Pubmed
,
Xenbase Ilves,
Long-term episomal maintenance of bovine papillomavirus type 1 plasmids is determined by attachment to host chromosomes, which Is mediated by the viral E2 protein and its binding sites.
1999,
Pubmed Lambert,
The genetics of bovine papillomavirus type 1.
1988,
Pubmed Lorca,
Fizzy is required for activation of the APC/cyclosome in Xenopus egg extracts.
1998,
Pubmed
,
Xenbase Lusky,
Genetic analysis of bovine papillomavirus type 1 trans-acting replication factors.
1985,
Pubmed Ma,
Interaction between cyclin-dependent kinases and human papillomavirus replication-initiation protein E1 is required for efficient viral replication.
1999,
Pubmed Malcles,
Regulation of bovine papillomavirus replicative helicase e1 by the ubiquitin-proteasome pathway.
2002,
Pubmed
,
Xenbase Pfleger,
Substrate recognition by the Cdc20 and Cdh1 components of the anaphase-promoting complex.
2001,
Pubmed
,
Xenbase Piirsoo,
Cis and trans requirements for stable episomal maintenance of the BPV-1 replicator.
1996,
Pubmed Rangasamy,
Bovine papillomavirus E1 protein is sumoylated by the host cell Ubc9 protein.
2000,
Pubmed Reimann,
Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex.
2001,
Pubmed
,
Xenbase Sedman,
The papillomavirus E1 protein forms a DNA-dependent hexameric complex with ATPase and DNA helicase activities.
1998,
Pubmed Skiadopoulos,
Bovine papillomavirus type 1 genomes and the E2 transactivator protein are closely associated with mitotic chromatin.
1998,
Pubmed Swindle,
Human papillomavirus DNA replication compartments in a transient DNA replication system.
1999,
Pubmed Ustav,
Transient replication of BPV-1 requires two viral polypeptides encoded by the E1 and E2 open reading frames.
1991,
Pubmed Wan,
Identification of multiple CDH1 homologues in vertebrates conferring different substrate specificities.
2001,
Pubmed zur Hausen,
Papillomaviruses and cancer: from basic studies to clinical application.
2002,
Pubmed
