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Plant Mol Biol
2009 Aug 01;706:681-92. doi: 10.1007/s11103-009-9500-3.
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OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints.
Aoyama T
,
Kobayashi T
,
Takahashi M
,
Nagasaka S
,
Usuda K
,
Kakei Y
,
Ishimaru Y
,
Nakanishi H
,
Mori S
,
Nishizawa NK
.
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Iron uptake and translocation in plants are important processes for both plant and human nutrition, whereas relatively little is known about the molecular mechanisms of iron transport within the plant body. Several reports have shown that yellow stripe 1 (YS1) and YS1-like (YSL) transporters mediate metal-phytosiderophore uptake and/or metal-nicotianamine translocation. Among the 18 YSL genes in rice (OsYSLs), OsYSL18 is predicted to encode a polypeptide of 679 amino acids containing 13 putative transmembrane domains. An OsYSL18-green fluorescent protein (GFP) fusion was localized to the plasma membrane when transiently expressed in onion epidermal cells. Electrophysiological measurements using Xenopus laevis oocytes showed that OsYSL18 transports iron(III)-deoxymugineic acid, but not iron(II)-nicotianamine, zinc(II)-deoxymugineic acid, or zinc(II)-nicotianamine. Reverse transcriptase PCR analysis revealed more OsYSL18 transcripts in flowers than in shoots or roots. OsYSL18 promoter-beta-glucuronidase (GUS) analysis revealed that OsYSL18 was expressed in reproductive organs including the pollen tube. In vegetative organs, OsYSL18 was specifically expressed in lamina joints, the inner cortex of crown roots, and phloem parenchyma and companion cells at the basal part of every leaf sheath. These results suggest that OsYSL18 is an iron-phytosiderophore transporter involved in the translocation of iron in reproductive organs and phloem in joints.
Fig. 1. Sequence characteristics of OsYSL18. a Phylogenetic relationships of YS1-like proteins in rice, maize, barley, and Arabidopsis. Circles represent the four subfamilies. Accession numbers: AB190912 for OsYSL1; AB126253 (AB164646) for OsYSL2; AB190913 for OsYSL3; AB190914 for OsYSL4; AB190915 for OsYSL5; AB190916 for OsYSL6; AB190917 for OsYSL7; AB190918 for OsYSL8; AB190919 for OsYSL9; AB190920 for OsYSL10; AB190921 for OsYSL11; AB190922 for OsYSL12; AB164644 for OsYSL13; AB164645 for OsYSL14; AB190923 for OsYSL15; AB190924 for OsYSL16; AB190925 for OsYSL17; AB190926 for OsYSL18; AF186234 for YS1; AB214183 for HvYS1; At4g24120 for AtYSL1; At5g24380 for AtYSL2; At5g53550 for AtYSL3; At5g41000 for AtYSL4; At3g17650 for AtYSL5; At3g27020 for AtYSL6; At1g65730 for AtYSL7; and At1g48370 for AtYSL8; b comparison of the putative amino acid sequences of OsYSL18 and YS1. White letters in black indicate identical amino acid residues. Lines indicate putative transmembrane domains of OsYSL18 and YS1. The boxed region represents the sequence determining structural properties and substrate specificity of YS1/YSL transporters as reported by Harada et al. (2007)
Fig. 2. Transporting activity of OsYSL18 analyzed by electrophysiological measurements using Xenopus laevis oocytes. The transport activities of Fe(III)–DMA, Fe(II)–NA, Zn(II)–DMA, and Zn(II)–NA were measured using the two-electrode voltage-clamp method. The oocytes were clamped at −80 mV, and steady-state currents in response to the addition of a metal–chelate complex (10 μl, 5 mM) were obtained. The data are mean ± SE of six independent oocytes injected with OsYSL18. The same number of water-injected oocytes was used as a control. N.D., not detected
Fig. 3. RT-PCR analysis of OsYSL18 expression. Total RNA from root (R), crown (C), shoot (S), or flower (F) grown under Fe-sufficient conditions was reverse-transcribed and amplified by PCR using primers specific to OsYSL18 or α-tubulin for the indicated number of cycles
Fig. 4. Subcellular localization of OsYSL18 expression. OsYSL18–GFP fusion protein (a–f) or GFP alone (g–i) was transiently expressed in onion epidermal cells and was observed by confocal laser scanning microscopy. (a, d, g) Overlay of confocal cross-sections and transmission images. PM, plasma membrane; T, tonoplast. (b, e, h) Confocal cross-sections. (c, f, i) Transmission images. (d–f) Magnified images of the boxed area shown in (a). Scale bars 20 μm
Fig. 5. Cellular localization of OsYSL18 expression in flowers and developing seeds during maturation as observed by histochemical staining of OsYSL18 promoter–GUS expression in transgenic rice plants. Before anthesis (a), just after fertilization (b), and 3 (c), 5 (d), 10 (e), 15 (f), and 30 (g) days after fertilization. (h, i) Magnified image just after fertilization. (j) Germinated pollen tube on in vitro culture medium. Shown are the results from a representative T1 line stained overnight
Fig. 6. Cellular localization of OsYSL18 expression in vegetative organs as observed by histochemical staining of OsYSL18 promoter–GUS expression in transgenic rice plants. (a) Expression in lamina joints. (b) Magnified image of (a). (c) Expression in crown roots. (d) Longitudinal section of crown roots. (e) Expression in the crown. (f) Longitudinal section around the basal parts of the leaf sheath. Three arrows indicate the positions of the cross-sections shown in (g–j). (g) Cross-section at the upper arrow of (f). The boxed area shows the magnified area in (h). (h–j) Magnified images of cross sections at the three arrows of (f). (h), upper arrow; (i),middle arrow; and (j) lower arrow. Intervals between the images are 600 μm. (k) Magnified image of a cross-section of a vascular bundle. Arrow, phloem companion cell; Xy, xylem. Shown are the results from a representative T1 line stained for 3 h for (a–d), 30 min for (e) and (f), and overnight for (g–k). Scale bars 1 mm for (f–j), and 50 μm for (k)
Bashir,
Cloning and characterization of deoxymugineic acid synthase genes from graminaceous plants.
2006, Pubmed
Bashir,
Cloning and characterization of deoxymugineic acid synthase genes from graminaceous plants.
2006,
Pubmed
Bughio,
Real-time [11C]methionine translocation in barley in relation to mugineic acid phytosiderophore biosynthesis.
2001,
Pubmed
Curie,
Maize yellow stripe1 encodes a membrane protein directly involved in Fe(III) uptake.
2001,
Pubmed
Curie,
Metal movement within the plant: contribution of nicotianamine and yellow stripe 1-like transporters.
2009,
Pubmed
,
Xenbase
DiDonato,
Arabidopsis Yellow Stripe-Like2 (YSL2): a metal-regulated gene encoding a plasma membrane transporter of nicotianamine-metal complexes.
2004,
Pubmed
Durrett,
The FRD3-mediated efflux of citrate into the root vasculature is necessary for efficient iron translocation.
2007,
Pubmed
,
Xenbase
Eide,
A novel iron-regulated metal transporter from plants identified by functional expression in yeast.
1996,
Pubmed
Gendre,
TcYSL3, a member of the YSL gene family from the hyper-accumulator Thlaspi caerulescens, encodes a nicotianamine-Ni/Fe transporter.
2007,
Pubmed
Grusak,
IMPROVING THE NUTRIENT COMPOSITION OF PLANTS TO ENHANCE HUMAN NUTRITION AND HEALTH1.
1999,
Pubmed
Harada,
Structural element responsible for the Fe(III)-phytosiderophore specific transport by HvYS1 transporter in barley.
2007,
Pubmed
,
Xenbase
Hell,
Iron uptake, trafficking and homeostasis in plants.
2003,
Pubmed
Hiei,
Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.
1994,
Pubmed
Higuchi,
Nicotianamine synthase gene expression differs in barley and rice under Fe-deficient conditions.
2001,
Pubmed
Igarashi,
Molecular cloning and characterization of a cDNA encoding proline transporter in rice.
2000,
Pubmed
,
Xenbase
Inoue,
Three rice nicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are expressed in cells involved in long-distance transport of iron and differentially regulated by iron.
2003,
Pubmed
Inoue,
Identification and localisation of the rice nicotianamine aminotransferase gene OsNAAT1 expression suggests the site of phytosiderophore synthesis in rice.
2008,
Pubmed
Inoue,
Rice OsYSL15 is an iron-regulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings.
2009,
Pubmed
,
Xenbase
Ishimaru,
Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+.
2006,
Pubmed
Jefferson,
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
1987,
Pubmed
Karimi,
GATEWAY vectors for Agrobacterium-mediated plant transformation.
2002,
Pubmed
Kiyomiya,
Light activates H2 15O flow in rice: Detailed monitoring using a positron-emitting tracer imaging system (PETIS).
2001,
Pubmed
Kiyomiya,
Real time visualization of 13N-translocation in rice under different environmental conditions using positron emitting Ttacer imaging system.
2001,
Pubmed
Koike,
OsYSL2 is a rice metal-nicotianamine transporter that is regulated by iron and expressed in the phloem.
2004,
Pubmed
,
Xenbase
Kosugi,
Upstream sequences of rice proliferating cell nuclear antigen (PCNA) gene mediate expression of PCNA-GUS chimeric gene in meristems of transgenic tobacco plants.
1991,
Pubmed
Le Jean,
A loss-of-function mutation in AtYSL1 reveals its role in iron and nicotianamine seed loading.
2005,
Pubmed
Mizuno,
Three nicotianamine synthase genes isolated from maize are differentially regulated by iron nutritional status.
2003,
Pubmed
Mori,
Iron acquisition by plants.
1999,
Pubmed
Murata,
A specific transporter for iron(III)-phytosiderophore in barley roots.
2006,
Pubmed
,
Xenbase
Nagasaka,
Time course analysis of gene expression over 24 hours in Fe-deficient barley roots.
2009,
Pubmed
Roberts,
Yellow stripe1. Expanded roles for the maize iron-phytosiderophore transporter.
2004,
Pubmed
Robinson,
A ferric-chelate reductase for iron uptake from soils.
1999,
Pubmed
Römheld,
Evidence for a specific uptake system for iron phytosiderophores in roots of grasses.
1986,
Pubmed
Schaaf,
ZmYS1 functions as a proton-coupled symporter for phytosiderophore- and nicotianamine-chelated metals.
2004,
Pubmed
,
Xenbase
Schaaf,
A putative function for the arabidopsis Fe-Phytosiderophore transporter homolog AtYSL2 in Fe and Zn homeostasis.
2005,
Pubmed
Shojima,
Biosynthesis of Phytosiderophores : In Vitro Biosynthesis of 2'-Deoxymugineic Acid from l-Methionine and Nicotianamine.
1990,
Pubmed
Stacey,
The Arabidopsis AtOPT3 protein functions in metal homeostasis and movement of iron to developing seeds.
2008,
Pubmed
Stacey,
Expression analyses of Arabidopsis oligopeptide transporters during seed germination, vegetative growth and reproduction.
2006,
Pubmed
Suzuki,
Deoxymugineic acid increases Zn translocation in Zn-deficient rice plants.
2008,
Pubmed
Takahashi,
Role of nicotianamine in the intracellular delivery of metals and plant reproductive development.
2003,
Pubmed
Tanaka,
NIP6;1 is a boric acid channel for preferential transport of boron to growing shoot tissues in Arabidopsis.
2008,
Pubmed
,
Xenbase
Tsukamoto,
(52)Fe translocation in barley as monitored by a positron-emitting tracer imaging system (PETIS): evidence for the direct translocation of Fe from roots to young leaves via phloem.
2009,
Pubmed
Von Wiren,
Iron Inefficiency in Maize Mutant ys1 (Zea mays L. cv Yellow-Stripe) Is Caused by a Defect in Uptake of Iron Phytosiderophores.
1994,
Pubmed
Waters,
Mutations in Arabidopsis yellow stripe-like1 and yellow stripe-like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds.
2006,
Pubmed
Yokosho,
OsFRDL1 is a citrate transporter required for efficient translocation of iron in rice.
2009,
Pubmed
,
Xenbase
von Wiren N,
Nicotianamine chelates both FeIII and FeII. Implications for metal transport in plants.
1999,
Pubmed