Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biophys J
2009 May 20;9610:4063-74. doi: 10.1016/j.bpj.2009.02.055.
Show Gene links
Show Anatomy links
The role of the C-terminus for functional heteromerization of the plant channel KDC1.
Naso A
,
Dreyer I
,
Pedemonte L
,
Testa I
,
Gomez-Porras JL
,
Usai C
,
Mueller-Rueber B
,
Diaspro A
,
Gambale F
,
Picco C
.
???displayArticle.abstract???
Voltage-gated potassium channels are formed by the assembly of four identical (homotetramer) or different (heterotetramer) subunits. Tetramerization of plant potassium channels involves the C-terminus of the protein. We investigated the role of the C-terminus of KDC1, a Shaker-like inward-rectifying K(+) channel that does not form functional homomeric channels, but participates in the formation of heteromeric complexes with other potassium alpha-subunits when expressed in Xenopus oocytes. The interaction of KDC1 with KAT1 was investigated using the yeast two-hybrid system, fluorescence and electrophysiological studies. We found that the KDC1-EGFP fusion protein is not targeted to the plasma membrane of Xenopus oocytes unless it is coexpressed with KAT1. Deletion mutants revealed that the KDC1 C-terminus is involved in heteromerization. Two domains of the C-terminus, the region downstream the putative cyclic nucleotide binding domain and the distal part of the C-terminus called K(HA) domain, contributed to a different extent to channel assembly. Whereas the first interacting region of the C-terminus was necessary for channel heteromerization, the removal of the distal K(HA) domain decreased but did not abolish the formation of heteromeric complexes. Similar results were obtained when coexpressing KDC1 with the KAT1-homolog KDC2 from carrots, thus indicating the physiological significance of the KAT1/KDC1 characterization. Electrophysiological experiments showed furthermore that the heteromerization capacity of KDC1 was negatively influenced by the presence of the enhanced green fluorescence protein fusion.
Ache,
VFK1, a Vicia faba K(+) channel involved in phloem unloading.
2001, Pubmed,
Xenbase
Ache,
VFK1, a Vicia faba K(+) channel involved in phloem unloading.
2001,
Pubmed
,
Xenbase
Baizabal-Aguirre,
Suppression of inward-rectifying K+ channels KAT1 and AKT2 by dominant negative point mutations in the KAT1 alpha-subunit.
1999,
Pubmed
Balduzzi,
Confocal microscopic study of GABA(A) receptors in Xenopus oocytes after rat brain mRNA injection: modulation by tyrosine kinase activity.
2001,
Pubmed
,
Xenbase
Bregante,
KDC1, a carrot Shaker-like potassium channel, reveals its role as a silent regulatory subunit when expressed in plant cells.
2008,
Pubmed
,
Xenbase
Daram,
Tetramerization of the AKT1 plant potassium channel involves its C-terminal cytoplasmic domain.
1997,
Pubmed
Downey,
KDC1, a novel carrot root hair K+ channel. Cloning, characterization, and expression in mammalian cells.
2000,
Pubmed
Dreyer,
A plant Shaker-like K+ channel switches between two distinct gating modes resulting in either inward-rectifying or "leak" current.
2001,
Pubmed
,
Xenbase
Dreyer,
Assembly of plant Shaker-like K(out) channels requires two distinct sites of the channel alpha-subunit.
2004,
Pubmed
,
Xenbase
Dreyer,
Plant K+ channel alpha-subunits assemble indiscriminately.
1997,
Pubmed
,
Xenbase
Duby,
AtKC1, a conditionally targeted Shaker-type subunit, regulates the activity of plant K+ channels.
2008,
Pubmed
Ehrhardt,
Association of plant K+(in) channels is mediated by conserved C-termini and does not affect subunit assembly.
1997,
Pubmed
Formentin,
KDC2, a functional homomeric potassium channel expressed during carrot embryogenesis.
2006,
Pubmed
,
Xenbase
Formentin,
DKT1, a novel K+ channel from carrot, forms functional heteromeric channels with KDC1.
2004,
Pubmed
,
Xenbase
Huth,
Fourier transformed spectral bio-imaging for studying the intracellular fate of liposomes.
2004,
Pubmed
Marten,
Voltage-dependent gating characteristics of the K+ channel KAT1 depend on the N and C termini.
1997,
Pubmed
Michard,
Inward rectification of the AKT2 channel abolished by voltage-dependent phosphorylation.
2005,
Pubmed
,
Xenbase
Michard,
A unique voltage sensor sensitizes the potassium channel AKT2 to phosphoregulation.
2005,
Pubmed
Mikosch,
Diacidic motif is required for efficient transport of the K+ channel KAT1 to the plasma membrane.
2006,
Pubmed
Naso,
Stoichiometry studies reveal functional properties of KDC1 in plant shaker potassium channels.
2006,
Pubmed
,
Xenbase
Ottolia,
Xenopus oocyte plasma membrane sheets for FRET analysis.
2007,
Pubmed
,
Xenbase
Paganetto,
A novel K+ channel expressed in carrot roots with a low susceptibility toward metal ions.
2001,
Pubmed
,
Xenbase
Picco,
Histidines are responsible for zinc potentiation of the current in KDC1 carrot channels.
2004,
Pubmed
,
Xenbase
Pilot,
Guard cell inward K+ channel activity in arabidopsis involves expression of the twin channel subunits KAT1 and KAT2.
2001,
Pubmed
,
Xenbase
Reintanz,
AtKC1, a silent Arabidopsis potassium channel alpha -subunit modulates root hair K+ influx.
2002,
Pubmed
Schroeder,
Involvement of ion channels and active transport in osmoregulation and signaling of higher plant cells.
1989,
Pubmed
Singer-Lahat,
Imaging plasma membrane proteins in large membrane patches of Xenopus oocytes.
2000,
Pubmed
,
Xenbase
Subramanian,
Intracellular trafficking/membrane targeting of human reduced folate carrier expressed in Xenopus oocytes.
2001,
Pubmed
,
Xenbase
Sutter,
Selective mobility and sensitivity to SNAREs is exhibited by the Arabidopsis KAT1 K+ channel at the plasma membrane.
2006,
Pubmed
,
Xenbase
Tsurui,
Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition.
2000,
Pubmed
Véry,
Molecular mechanisms and regulation of K+ transport in higher plants.
2003,
Pubmed
Xicluna,
Increased functional diversity of plant K+ channels by preferential heteromerization of the shaker-like subunits AKT2 and KAT2.
2007,
Pubmed
,
Xenbase
Yu,
Phosphorylation of SPICK2, an AKT2 channel homologue from Samanea motor cells.
2006,
Pubmed
Zampighi,
Functional and morphological correlates of connexin50 expressed in Xenopus laevis oocytes.
1999,
Pubmed
,
Xenbase
Zimmermann,
The K+ channel SKT1 is co-expressed with KST1 in potato guard cells--both channels can co-assemble via their conserved KT domains.
2001,
Pubmed
,
Xenbase
Zimmermann,
Spectral imaging and its applications in live cell microscopy.
2003,
Pubmed
van der Ven,
Indications for a novel muscular dystrophy pathway. gamma-filamin, the muscle-specific filamin isoform, interacts with myotilin.
2000,
Pubmed