Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Blood Cells Mol Dis
2009 Jan 01;423:233-40. doi: 10.1016/j.bcmd.2009.01.021.
Show Gene links
Show Anatomy links
Chemical crosslinking studies with the mouse Kcc1 K-Cl cotransporter.
Casula S
,
Zolotarev AS
,
Stuart-Tilley AK
,
Wilhelm S
,
Shmukler BE
,
Brugnara C
,
Alper SL
.
???displayArticle.abstract???
Oligomerization, function, and regulation of unmodified mouse Kcc1 K-Cl cotransporter were studied by chemical crosslinking. Treatment of Xenopus oocytes and 293T cells expressing K-Cl cotransporter Kcc1 with several types of chemical cross-linkers shifted Kcc1 polypeptide to higher molecular weight forms. More extensive studies were performed with the amine-reactive disuccinyl suberate (DSS) and with the sulfhydryl-reactive bis-maleimidohexane (BMH). Kcc1 cross-linking was time-dependent in intact oocytes, and was independent of protein concentration in detergent lysates from oocytes or 293T cells. Kcc1 cross-linking by the cleavable cross-linker DTME was reversible. The N-terminal and C-terminal cytoplasmic tails of Kcc1 were not essential for Kcc1 crosslinking. PFO-PAGE and gel filtration revealed oligomeric states of uncrosslinked KCC1 corresponding in mobility to that of cross-linked protein. DSS and BMH each inhibited KCC1-mediated (86)Rb(+) uptake stimulated by hypotonicity or by N-ethylmaleimide (NEM) without reduction in nominal surface abundance of KCC1. These data add to evidence supporting the oligomeric state of KCC polypeptides.
Boettger,
Deafness and renal tubular acidosis in mice lacking the K-Cl co-transporter Kcc4.
2002, Pubmed
Boettger,
Deafness and renal tubular acidosis in mice lacking the K-Cl co-transporter Kcc4.
2002,
Pubmed
Brugnara,
Novel therapies for prevention of erythrocyte dehydration in sickle cell anemia.
2001,
Pubmed
Caron,
Cloning and functional characterization of a cation-Cl- cotransporter-interacting protein.
2000,
Pubmed
,
Xenbase
Casula,
A dominant negative mutant of the KCC1 K-Cl cotransporter: both N- and C-terminal cytoplasmic domains are required for K-Cl cotransport activity.
2001,
Pubmed
,
Xenbase
Delpire,
SPAK and OSR1: STE20 kinases involved in the regulation of ion homoeostasis and volume control in mammalian cells.
2008,
Pubmed
Gamba,
Molecular physiology and pathophysiology of electroneutral cation-chloride cotransporters.
2005,
Pubmed
Hankins,
Phase I study of magnesium pidolate in combination with hydroxycarbamide for children with sickle cell anaemia.
2008,
Pubmed
Hayashi,
A slow pH-dependent conformational transition underlies a novel mode of activation of the epithelial Na+/H+ exchanger-3 isoform.
2002,
Pubmed
Howard,
The K-Cl cotransporter KCC3 is mutant in a severe peripheral neuropathy associated with agenesis of the corpus callosum.
2002,
Pubmed
,
Xenbase
Hübner,
Disruption of KCC2 reveals an essential role of K-Cl cotransport already in early synaptic inhibition.
2001,
Pubmed
Jennings,
Volume-sensitive K(+)/Cl(-) cotransport in rabbit erythrocytes. Analysis of the rate-limiting activation and inactivation events.
1999,
Pubmed
Kahle,
Molecular physiology of the WNK kinases.
2008,
Pubmed
Kinsella,
Na+/H+ exchanger: proton modifier site regulation of activity.
1998,
Pubmed
Lacroix,
Kinetic analysis of the regulation of the Na+/H+ exchanger NHE-1 by osmotic shocks.
2008,
Pubmed
Lauf,
K-Cl cotransport in red blood cells from patients with KCC3 isoform mutants.
2006,
Pubmed
Lauf,
K+:Cl- cotransport: sulfhydryls, divalent cations, and the mechanism of volume activation in a red cell.
1985,
Pubmed
Lauf,
K-Cl cotransport: properties and molecular mechanism.
2000,
Pubmed
,
Xenbase
Moore-Hoon,
The structural unit of the secretory Na+-K+-2Cl- cotransporter (NKCC1) is a homodimer.
2000,
Pubmed
Parker,
Glutaraldehyde fixation of sodium transport in dog red blood cells.
1984,
Pubmed
Peters,
Mild spherocytosis and altered red cell ion transport in protein 4. 2-null mice.
1999,
Pubmed
Plata,
Isoforms of the Na-K-2Cl cotransporter in murine TAL II. Functional characterization and activation by cAMP.
1999,
Pubmed
,
Xenbase
Popov,
Transmembrane folding of the human erythrocyte anion exchanger (AE1, Band 3) determined by scanning and insertional N-glycosylation mutagenesis.
1999,
Pubmed
Ramjeesingh,
Novel method for evaluation of the oligomeric structure of membrane proteins.
1999,
Pubmed
,
Xenbase
Ramjeesingh,
Quaternary structure of the chloride channel ClC-2.
2000,
Pubmed
Ramjeesingh,
A monomer is the minimum functional unit required for channel and ATPase activity of the cystic fibrosis transmembrane conductance regulator.
2001,
Pubmed
Richardson,
The regulation of salt transport and blood pressure by the WNK-SPAK/OSR1 signalling pathway.
2008,
Pubmed
Rust,
Disruption of erythroid K-Cl cotransporters alters erythrocyte volume and partially rescues erythrocyte dehydration in SAD mice.
2007,
Pubmed
Simard,
Homooligomeric and heterooligomeric associations between K+-Cl- cotransporter isoforms and between K+-Cl- and Na+-K+-Cl- cotransporters.
2007,
Pubmed
,
Xenbase
Su,
Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and functional regulation.
1999,
Pubmed
,
Xenbase
Watty,
Fixation of allosteric states of the nicotinic acetylcholine receptor by chemical cross-linking.
1997,
Pubmed
,
Xenbase
Zolotarev,
AE2 anion exchanger polypeptide is a homooligomer in pig gastric membranes: a chemical cross-linking study.
1999,
Pubmed