Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Am J Physiol Regul Integr Comp Physiol
2009 May 01;2965:R1512-7. doi: 10.1152/ajpregu.90901.2008.
Show Gene links
Show Anatomy links
Effect of physiological levels of caffeine on Ca2+ handling and fatigue development in Xenopus isolated single myofibers.
Rosser JI
,
Walsh B
,
Hogan MC
.
???displayArticle.abstract???
The purpose of the present study was to determine whether exposure to exogenous physiological concentrations of caffeine influence contractility, Ca(2+) handling, and fatigue development in isolated single Xenopus laevis skeletal muscle fibers. After isolation, two identical contractile periods (separated by 60-min rest) were conducted in each single myofiber (n = 8) at 20 degrees C. During the first contractile period, four fibers were perfused with a noncaffeinated Ringer solution, while the other four fibers were perfused with a caffeinated (70 microM) Ringer solution. The order was reversed for the second contractile period. The single myofibers were stimulated during each contractile period at increasing frequencies (0.16, 0.20, 0.25, 0.33, 0.50, and 1.0 tetanic contractions/s), with each stimulation frequency lasting 2 min until fatigue ensued, defined in this study as a fall in tension development to 66% of maximum. Tension development and free cytosolic [Ca(2+)] (fura-2 fluorescence spectroscopy) were simultaneously measured. There was no significant difference in the peak force generation, time to fatigue, cytosolic Ca(2+) levels, or relaxation times between the noncaffeinated and caffeinated trials. These results demonstrate that physiological levels of caffeine have no significant effect on Xenopus single myofiber contractility, Ca(2+) handling, and fatigue development, and suggest that any ergogenic effects of physiological levels of caffeine on muscle performance during contractions of moderate to high intensity are likely related to factors extraneous to the muscle fiber.
Allen,
The effects of caffeine on intracellular calcium, force and the rate of relaxation of mouse skeletal muscle.
1995, Pubmed
Allen,
The effects of caffeine on intracellular calcium, force and the rate of relaxation of mouse skeletal muscle.
1995,
Pubmed
Allen,
Impaired calcium release during fatigue.
2008,
Pubmed
Allen,
Intracellular calcium and tension during fatigue in isolated single muscle fibres from Xenopus laevis.
1989,
Pubmed
,
Xenbase
Beck,
The acute effects of a caffeine-containing supplement on strength, muscular endurance, and anaerobic capabilities.
2006,
Pubmed
Beck,
The acute effects of a caffeine-containing supplement on bench press strength and time to running exhaustion.
2008,
Pubmed
Chesley,
Regulation of muscle glycogenolytic flux during intense aerobic exercise after caffeine ingestion.
1998,
Pubmed
Costill,
Effects of caffeine ingestion on metabolism and exercise performance.
1978,
Pubmed
Crowe,
Physiological and cognitive responses to caffeine during repeated, high-intensity exercise.
2006,
Pubmed
Davis,
Central nervous system effects of caffeine and adenosine on fatigue.
2003,
Pubmed
Delay,
Caffeine potentiation of calcium release in frog skeletal muscle fibres.
1986,
Pubmed
Fitts,
Cellular mechanisms of muscle fatigue.
1994,
Pubmed
Fredholm,
[Connection between caffeine, adenosine receptors and dopamine. Coffee reduces the risk of Parkinson disease].
2004,
Pubmed
Fryer,
Actions of caffeine on fast- and slow-twitch muscles of the rat.
1989,
Pubmed
Germinario,
Effects of modulators of sarcoplasmic Ca2+ release on the development of skeletal muscle fatigue.
2004,
Pubmed
Graham,
Metabolic, catecholamine, and exercise performance responses to various doses of caffeine.
1995,
Pubmed
Graham,
Caffeine, coffee and ephedrine: impact on exercise performance and metabolism.
2001,
Pubmed
Graham,
Performance and metabolic responses to a high caffeine dose during prolonged exercise.
1991,
Pubmed
Greer,
Caffeine, performance, and metabolism during repeated Wingate exercise tests.
1998,
Pubmed
Greer,
Wingate performance and surface EMG frequency variables are not affected by caffeine ingestion.
2006,
Pubmed
Greer,
Comparison of caffeine and theophylline ingestion: exercise metabolism and endurance.
2000,
Pubmed
Herrmann-Frank,
Caffeine and excitation-contraction coupling in skeletal muscle: a stimulating story.
1999,
Pubmed
Howlett,
Caffeine administration results in greater tension development in previously fatigued canine muscle in situ.
2005,
Pubmed
Huang,
Adenosine A2A, but not A1, receptors mediate the arousal effect of caffeine.
2005,
Pubmed
James,
70 microM caffeine treatment enhances in vitro force and power output during cyclic activities in mouse extensor digitorum longus muscle.
2005,
Pubmed
James,
Effects of caffeine on mouse skeletal muscle power output during recovery from fatigue.
2004,
Pubmed
Jones,
Low-frequency fatigue in isolated skeletal muscles and the effects of methylxanthines.
1982,
Pubmed
Kalmar,
Effects of caffeine on neuromuscular function.
1999,
Pubmed
Kindig,
Effect of dissociating cytosolic calcium and metabolic rate on intracellular PO2 kinetics in single frog myocytes.
2005,
Pubmed
,
Xenbase
Kong,
Caffeine induces Ca2+ release by reducing the threshold for luminal Ca2+ activation of the ryanodine receptor.
2008,
Pubmed
Lee,
Changes in tetanic and resting [Ca2+]i during fatigue and recovery of single muscle fibres from Xenopus laevis.
1991,
Pubmed
,
Xenbase
Lynge,
Distribution of adenosine A1, A2A and A2B receptors in human skeletal muscle.
2000,
Pubmed
Meyers,
Caffeine increases time to fatigue by maintaining force and not by altering firing rates during submaximal isometric contractions.
2005,
Pubmed
Mohr,
Caffeine ingestion and metabolic responses of tetraplegic humans during electrical cycling.
1998,
Pubmed
Nehlig,
Caffeine and the central nervous system: mechanisms of action, biochemical, metabolic and psychostimulant effects.
1992,
Pubmed
Pagala,
Potassium and caffeine contractures of mouse muscles before and after fatiguing stimulation.
1994,
Pubmed
Paluska,
Caffeine and exercise.
2003,
Pubmed
Plaskett,
Caffeine increases endurance and attenuates force sensation during submaximal isometric contractions.
2001,
Pubmed
Reading,
Positive inotropism in mammalian skeletal muscle in vitro during and after fatigue.
2004,
Pubmed
Rousseau,
Activation of the Ca2+ release channel of skeletal muscle sarcoplasmic reticulum by caffeine and related compounds.
1988,
Pubmed
Spriet,
Caffeine ingestion and muscle metabolism during prolonged exercise in humans.
1992,
Pubmed
Stary,
Impairment of Ca(2+) release in single Xenopus muscle fibers fatigued at varied extracellular PO(2).
2000,
Pubmed
,
Xenbase
Stuart,
Multiple effects of caffeine on simulated high-intensity team-sport performance.
2005,
Pubmed
Tarnopolsky,
Effect of caffeine on the neuromuscular system--potential as an ergogenic aid.
2008,
Pubmed
Tarnopolsky,
Caffeine potentiates low frequency skeletal muscle force in habitual and nonhabitual caffeine consumers.
2000,
Pubmed
Westerblad,
Functional significance of Ca2+ in long-lasting fatigue of skeletal muscle.
2000,
Pubmed
Westerblad,
Tension restoration with caffeine in fatigued Xenopus muscle fibres of various types.
1987,
Pubmed
,
Xenbase
Westerblad,
Changes of myoplasmic calcium concentration during fatigue in single mouse muscle fibers.
1991,
Pubmed
Wiles,
The effects of caffeine ingestion on performance time, speed and power during a laboratory-based 1 km cycling time-trial.
2006,
Pubmed