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J Biol Chem
2008 Nov 21;28347:32236-43. doi: 10.1074/jbc.M806636200.
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Histone N-terminal tails interfere with nucleosome traversal by RNA polymerase II.
Ujvári A
,
Hsieh FK
,
Luse SW
,
Studitsky VM
,
Luse DS
.
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We determined the effect of the N-terminal histone tails on nucleosome traversal by yeast and human RNA polymerase II (pol II). Removal of H2A/H2B tails, H3/H4 tails, or all tails increased complete traversal of the nucleosome by human pol II, although the increase varied considerably depending on the template and on which tails were removed. Human pol II achieved >80% traversal of one nucleosomal template lacking the H2A/H2B tails, but even in those reactions, the transcript elongation rate was lower than the rate on pure DNA templates. For yeast pol II, transcription proceeded much farther into the nucleosome in the absence of tails, but complete read-through was not substantially increased by tail removal. Transcription factor IIS provided roughly the same level of read-through stimulation for transcript elongation in the presence or absence of tails. FACT also stimulated elongation on nucleosomal templates, and this effect was similar regardless of the presence of tails. For both polymerases, removal of the H2A/H2B tails reduced pausing throughout the nucleosome, suggesting that histone tails affect a common step at most points during nucleosome traversal. We conclude that histone tails provide a significant part of the nucleosomal barrier to pol II transcript elongation.
Ausio,
Use of selectively trypsinized nucleosome core particles to analyze the role of the histone "tails" in the stabilization of the nucleosome.
1989, Pubmed
Ausio,
Use of selectively trypsinized nucleosome core particles to analyze the role of the histone "tails" in the stabilization of the nucleosome.
1989,
Pubmed
Awrey,
Transcription elongation through DNA arrest sites. A multistep process involving both RNA polymerase II subunit RPB9 and TFIIS.
1997,
Pubmed
Bancaud,
Nucleosome chiral transition under positive torsional stress in single chromatin fibers.
2007,
Pubmed
Belotserkovskaya,
FACT facilitates transcription-dependent nucleosome alteration.
2003,
Pubmed
Bertin,
H2A and H2B tails are essential to properly reconstitute nucleosome core particles.
2007,
Pubmed
,
Xenbase
Bondarenko,
Nucleosomes can form a polar barrier to transcript elongation by RNA polymerase II.
2006,
Pubmed
Brower-Toland,
Specific contributions of histone tails and their acetylation to the mechanical stability of nucleosomes.
2005,
Pubmed
Carruthers,
The core histone N termini function independently of linker histones during chromatin condensation.
2000,
Pubmed
Dyer,
Reconstitution of nucleosome core particles from recombinant histones and DNA.
2004,
Pubmed
Fischle,
Histone and chromatin cross-talk.
2003,
Pubmed
Fletcher,
Core histone tail domains mediate oligonucleosome folding and nucleosomal DNA organization through distinct molecular mechanisms.
1995,
Pubmed
Garcia-Ramirez,
Role of the histone "tails" in the folding of oligonucleosomes depleted of histone H1.
1992,
Pubmed
Hayes,
Histone contributions to the structure of DNA in the nucleosome.
1991,
Pubmed
,
Xenbase
Hill,
Core histone-DNA interactions in sea urchin sperm chromatin. The N-terminal tail of H2B interacts with linker DNA.
1990,
Pubmed
Izban,
Transcription on nucleosomal templates by RNA polymerase II in vitro: inhibition of elongation with enhancement of sequence-specific pausing.
1991,
Pubmed
,
Xenbase
Kan,
The H3 tail domain participates in multiple interactions during folding and self-association of nucleosome arrays.
2007,
Pubmed
,
Xenbase
Kettenberger,
Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS.
2004,
Pubmed
Kireeva,
Nucleosome remodeling induced by RNA polymerase II: loss of the H2A/H2B dimer during transcription.
2002,
Pubmed
Kireeva,
Nature of the nucleosomal barrier to RNA polymerase II.
2005,
Pubmed
Kouzarides,
Chromatin modifications and their function.
2007,
Pubmed
Lee,
The N-terminal tail of histone H2A binds to two distinct sites within the nucleosome core.
1997,
Pubmed
,
Xenbase
Li,
Rapid spontaneous accessibility of nucleosomal DNA.
2005,
Pubmed
Lowary,
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning.
1998,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Luger,
Characterization of nucleosome core particles containing histone proteins made in bacteria.
1997,
Pubmed
,
Xenbase
Luger,
Preparation of nucleosome core particle from recombinant histones.
1999,
Pubmed
,
Xenbase
Mellor,
Dynamic nucleosomes and gene transcription.
2006,
Pubmed
Morse,
Effect of trypsinization and histone H5 addition on DNA twist and topology in reconstituted minichromosomes.
1986,
Pubmed
Rhoades,
Structural features of nucleosomes reorganized by yeast FACT and its HMG box component, Nhp6.
2004,
Pubmed
Schones,
Dynamic regulation of nucleosome positioning in the human genome.
2008,
Pubmed
Sivolob,
Nucleosome dynamics. VI. Histone tail regulation of tetrasome chiral transition. A relaxation study of tetrasomes on DNA minicircles.
2000,
Pubmed
Studitsky,
Preparation and analysis of positioned nucleosomes.
1999,
Pubmed
Thåström,
Sequence motifs and free energies of selected natural and non-natural nucleosome positioning DNA sequences.
1999,
Pubmed
Tse,
Hybrid trypsinized nucleosomal arrays: identification of multiple functional roles of the H2A/H2B and H3/H4 N-termini in chromatin fiber compaction.
1997,
Pubmed
Widlund,
DNA sequence-dependent contributions of core histone tails to nucleosome stability: differential effects of acetylation and proteolytic tail removal.
2000,
Pubmed
Zhang,
Combinatorial control of human RNA polymerase II (RNAP II) pausing and transcript cleavage by transcription factor IIF, hepatitis delta antigen, and stimulatory factor II.
2003,
Pubmed
Zheng,
Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.
2003,
Pubmed
,
Xenbase