Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
CK2alpha/CK1alpha chimeras are sensitive to regulation by the CK2beta subunit.
Jedlicki A
,
Allende CC
,
Allende JE
.
???displayArticle.abstract???
The effect of CK2beta on the activity of CK2alpha and other protein kinases that can bind this regulatory subunit is not fully understood. In an attempt to improve our understanding of this effect, chimeras of CK2alpha and CK1alpha have been constructed. These chimeras contain different portions of the CK2alpha amino terminal region that are involved in the interaction with CK2beta to form CK2 tetramers. In the case of chimeras 1 and 2, the portions of CK2alpha replace the corresponding segments of CK1alpha. In the case of chimera 3, the fragment of CK2alpha is added to the whole CK1alpha molecule with the exception of the initial methionine. Chimera 3 has 8% of the activity of CK1alphaWT, while chimeras 1 and 2 are 3 orders of magnitude less active than CK1alphaWT. All three chimeras bind tightly to CK2beta, but only chimeras 1 and 2 are significantly stimulated in their capacity to phosphorylate casein and canonical peptide substrates by addition of the regulatory subunit. No stimulation was observed with phosvitin or non-canonical peptides derived from beta-catenin. CK2beta protects chimeras 1 and 2 from thermal inactivation. Chimera 2 can phosphorylate CK2beta and autophosphorylate; however, salt concentrations above 150 mM NaCl eliminate the phosphorylation of CK2beta but not the autophosphorylation of chimera 2. Similarly, high salt decrease the stimulatory effect of CK2beta on the phosphorylation of casein.
Allende,
Protein kinases. 4. Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation.
1995, Pubmed
Allende,
Protein kinases. 4. Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation.
1995,
Pubmed
Allende,
Promiscuous subunit interactions: a possible mechanism for the regulation of protein kinase CK2.
1998,
Pubmed
Bibby,
The multiple personalities of the regulatory subunit of protein kinase CK2: CK2 dependent and CK2 independent roles reveal a secret identity for CK2beta.
2005,
Pubmed
Boldyreff,
Reconstitution of normal and hyperactivated forms of casein kinase-2 by variably mutated beta-subunits.
1993,
Pubmed
Boldyreff,
Efficient autophosphorylation and phosphorylation of the beta-subunit by casein kinase-2 require the integrity of an acidic cluster 50 residues downstream from the phosphoacceptor site.
1994,
Pubmed
Burzio,
Biochemical and cellular characteristics of the four splice variants of protein kinase CK1alpha from zebrafish (Danio rerio).
2002,
Pubmed
Bustos,
Generation of protein kinase Ck1alpha mutants which discriminate between canonical and non-canonical substrates.
2005,
Pubmed
Chong,
Mechanisms of regulating the Raf kinase family.
2003,
Pubmed
Cosmelli,
An inactive mutant of the alpha subunit of protein kinase CK2 that traps the regulatory CK2beta subunit.
1997,
Pubmed
,
Xenbase
English,
Identification of substrates and regulators of the mitogen-activated protein kinase ERK5 using chimeric protein kinases.
1998,
Pubmed
Flotow,
Phosphate groups as substrate determinants for casein kinase I action.
1990,
Pubmed
Guerra,
Protein kinase CK2 and its role in cellular proliferation, development and pathology.
1999,
Pubmed
Guerra,
Modulation of human checkpoint kinase Chk1 by the regulatory beta-subunit of protein kinase CK2.
2003,
Pubmed
Hanks,
Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification.
1995,
Pubmed
Hinrichs,
Activity of recombinant alpha and beta subunits of casein kinase II from Xenopus laevis.
1993,
Pubmed
,
Xenbase
Jedlicki,
The cDNAs coding for the alpha- and beta-subunits of Xenopus laevis casein kinase II.
1992,
Pubmed
,
Xenbase
Kusk,
Interactions of protein kinase CK2beta subunit within the holoenzyme and with other proteins.
1999,
Pubmed
Lieberman,
CK2 beta, which inhibits Mos function, binds to a discrete domain in the N-terminus of Mos.
2004,
Pubmed
,
Xenbase
Litchfield,
Phosphorylation of the beta subunit of casein kinase II in human A431 cells. Identification of the autophosphorylation site and a site phosphorylated by p34cdc2.
1991,
Pubmed
Liu,
Src-Abl tyrosine kinase chimeras: replacement of the adenine binding pocket of c-Abl with v-Src to swap nucleotide and inhibitor specificities.
2000,
Pubmed
Marin,
A noncanonical sequence phosphorylated by casein kinase 1 in beta-catenin may play a role in casein kinase 1 targeting of important signaling proteins.
2003,
Pubmed
Marin,
Structural features underlying the unusual mode of calmodulin phosphorylation by protein kinase CK2: A study with synthetic calmodulin fragments.
1999,
Pubmed
Niefind,
Primary and secondary interactions between CK2alpha and CK2beta lead to ring-like structures in the crystals of the CK2 holoenzyme.
2005,
Pubmed
Niefind,
Crystal structure of human protein kinase CK2: insights into basic properties of the CK2 holoenzyme.
2001,
Pubmed
Pagano,
Autophosphorylation at the regulatory beta subunit reflects the supramolecular organization of protein kinase CK2.
2005,
Pubmed
Pinna,
Protein kinase CK2: a challenge to canons.
2002,
Pubmed
Rodríguez,
Protein kinase casein kinase 2 holoenzyme produced ectopically in human cells can be exported to the external side of the cellular membrane.
2005,
Pubmed
Sarrouilhe,
The tight association of protein kinase CK2 with plasma membranes is mediated by a specific domain of its regulatory beta-subunit.
1998,
Pubmed
Tapia,
Role of the carboxyl terminus on the catalytic activity of protein kinase CK2alpha subunit.
2002,
Pubmed
,
Xenbase
Tapia,
Cell cycle regulatory protein p27KIP1 is a substrate and interacts with the protein kinase CK2.
2004,
Pubmed
,
Xenbase
Valero,
Quaternary structure of casein kinase 2. Characterization of multiple oligomeric states and relation with its catalytic activity.
1995,
Pubmed
Yue,
Mechanistic studies of the mitotic activation of Mos.
2006,
Pubmed
,
Xenbase