Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Plant Mol Biol
2003 Nov 01;535:633-45. doi: 10.1023/B:PLAN.0000019070.60954.77.
Show Gene links
Show Anatomy links
Members of the aquaporin family in the developing pea seed coat include representatives of the PIP, TIP, and NIP subfamilies.
Schuurmans JA
,
van Dongen JT
,
Rutjens BP
,
Boonman A
,
Pieterse CM
,
Borstlap AC
.
???displayArticle.abstract???
Water and nutrients required by developing seeds are mainly supplied by the phloem and have to be released from a maternal parenchyma tissue before being utilized by the filial tissues of embryo and endosperm. To identify aquaporins that could be involved in this process four full-length cDNAs were cloned and sequenced from a cDNA library of developing seed coats of pea (Pisum sativum L.). The cDNA of PsPIP1-1 appeared to be identical to that of clone 7a/TRG-31, a turgor-responsive gene cloned previously from pea roots. PsPIP1-1, PsPIP2-1, and PsTIP1-1, or their possible close homologues, were also expressed in cotyledons of developing and germinating seeds, and in roots and shoots of seedlings, but transcripts of PsNIP-1 were only detected in the seed coat. In mature dry seeds, high hybridization signals were observed with the probe for PsPIP1-1, but transcripts of PsPIP2-1, PsTIP1-1, and PsNIP-1 were not detected. Functional characterization after heterologous expression in Xenopus oocytes showed that PsPIP2-1 and PsTIP1-1 are aquaporins whereas PsNIP-1 is an aquaglyceroporin. PsNIP-1, like several other NIPs, contains a tryptophan residue corresponding with Trp-48 in GlpF (the glycerol facilitator of Escherichia coli) that borders the selectivity filter in the permeation channel. It is suggested that PsPIP1-1 and/or its possible close homologues could play a role in water absorption during seed imbibition, and that PsPIP2-1, possibly together with PsPIP1-1, could be involved in the release of phloem water from the seed coat symplast, which is intimately connected with the release of nutrients for the embryo.
Agre,
The aquaporins, blueprints for cellular plumbing systems.
1998, Pubmed
Agre,
The aquaporins, blueprints for cellular plumbing systems.
1998,
Pubmed
Aharon,
Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress.
2003,
Pubmed
Altschul,
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
1997,
Pubmed
Barrieu,
High expression of the tonoplast aquaporin ZmTIP1 in epidermal and conducting tissues of maize.
1998,
Pubmed
Biela,
The Nicotiana tabacum plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol.
1999,
Pubmed
,
Xenbase
Borgnia,
Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels from Escherichia coli.
2001,
Pubmed
Borstlap,
Early diversification of plant aquaporins.
2002,
Pubmed
Chaumont,
Plasma membrane intrinsic proteins from maize cluster in two sequence subgroups with differential aquaporin activity.
2000,
Pubmed
,
Xenbase
Chaumont,
Aquaporins constitute a large and highly divergent protein family in maize.
2001,
Pubmed
,
Xenbase
Chen,
Inhibiting expression of a tomato ripening-associated membrane protein increases organic acids and reduces sugar levels of fruit.
2001,
Pubmed
Ciavatta,
An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine.
2001,
Pubmed
,
Xenbase
Corpet,
Multiple sequence alignment with hierarchical clustering.
1988,
Pubmed
Daniels,
The plasma membrane of Arabidopsis thaliana contains a mercury-insensitive aquaporin that is a homolog of the tonoplast water channel protein TIP.
1994,
Pubmed
,
Xenbase
De Jong,
The Mechanism of Amino Acid Efflux from Seed Coats of Developing Pea Seeds as Revealed by Uptake Experiments.
1997,
Pubmed
Dean,
Purification and functional reconstitution of soybean nodulin 26. An aquaporin with water and glycerol transport properties.
1999,
Pubmed
,
Xenbase
Doering-Saad,
Use of aphid stylectomy and RT-PCR for the detection of transporter mRNAs in sieve elements.
2002,
Pubmed
Dordas,
Permeability and channel-mediated transport of boric acid across membrane vesicles isolated from squash roots.
2000,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Frangne,
Expression and distribution of a vaculoar aquaporin in young and mature leaf tissues of Brassica napus in relation to water fluxes.
2001,
Pubmed
Froger,
Prediction of functional residues in water channels and related proteins.
1998,
Pubmed
Fu,
Structure of a glycerol-conducting channel and the basis for its selectivity.
2000,
Pubmed
Gao,
Characterization and expression of plasma and tonoplast membrane aquaporins in primed seed of Brassica napus during germination under stress conditions.
1999,
Pubmed
Gerbeau,
Aquaporin Nt-TIPa can account for the high permeability of tobacco cell vacuolar membrane to small neutral solutes.
1999,
Pubmed
,
Xenbase
Guenther,
Water-selective and multifunctional aquaporins from Lotus japonicus nodules.
2000,
Pubmed
,
Xenbase
Guerrero,
Turgor-responsive gene transcription and RNA levels increase rapidly when pea shoots are wilted. Sequence and expression of three inducible genes.
1990,
Pubmed
Guerrero,
Tissue-specific expression of a plant turgor-responsive gene with amino acid sequence homology to transport-facilitating proteins.
1993,
Pubmed
Hakman,
High expression of putative aquaporin genes in cells with transporting and nutritive functions during seed development in Norway spruce (Picea abies).
2002,
Pubmed
Heller,
Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli.
1980,
Pubmed
Heymann,
Aquaporins: Phylogeny, Structure, and Physiology of Water Channels.
1999,
Pubmed
Ikeda,
Characterization of aquaporin-6 as a nitrate channel in mammalian cells. Requirement of pore-lining residue threonine 63.
2002,
Pubmed
,
Xenbase
Jauh,
Tonoplast intrinsic protein isoforms as markers for vacuolar functions.
1999,
Pubmed
Johanson,
The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants.
2001,
Pubmed
Jones,
Developmental expression of a turgor-responsive gene that encodes an intrinsic membrane protein.
1995,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Lagrée,
Switch from an aquaporin to a glycerol channel by two amino acids substitution.
1999,
Pubmed
,
Xenbase
Liu,
Isolation and expression analysis of two rice genes encoding the major intrinsic protein.
1994,
Pubmed
Logemann,
Improved method for the isolation of RNA from plant tissues.
1987,
Pubmed
Ludevid,
The Expression Pattern of the Tonoplast Intrinsic Protein gamma-TIP in Arabidopsis thaliana Is Correlated with Cell Enlargement.
1992,
Pubmed
Martre,
Plasma membrane aquaporins play a significant role during recovery from water deficit.
2002,
Pubmed
Maurel,
Function and regulation of seed aquaporins.
1997,
Pubmed
,
Xenbase
Moshelion,
Plasma membrane aquaporins in the motor cells of Samanea saman: diurnal and circadian regulation.
2002,
Pubmed
,
Xenbase
Opperman,
Root-knot nematode--directed expression of a plant root--specific gene.
1994,
Pubmed
Ouyang,
Protein phosphorylation stimulates the rate of malate uptake across the peribacteroid membrane of soybean nodules.
1991,
Pubmed
Patrick,
Compartmentation of transport and transfer events in developing seeds.
2001,
Pubmed
Shackel,
Seed coat cell turgor in chickpea is independent of changes in plant and pod water potential.
2000,
Pubmed
Siefritz,
PIP1 plasma membrane aquaporins in tobacco: from cellular effects to function in plants.
2002,
Pubmed
,
Xenbase
Silberberg,
Voltage-induced slow activation and deactivation of mechanosensitive channels in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Suga,
Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporins in radish organs.
2001,
Pubmed
Sui,
Structural basis of water-specific transport through the AQP1 water channel.
,
Pubmed
Tsukaguchi,
Molecular characterization of a broad selectivity neutral solute channel.
1998,
Pubmed
,
Xenbase
Udvardi,
METABOLITE TRANSPORT ACROSS SYMBIOTIC MEMBRANES OF LEGUME NODULES.
1997,
Pubmed
Van Dongen,
Structure of the developing pea seed coat and the post-phloem transport pathway of nutrients.
2003,
Pubmed
Wallace,
Functional selectivity for glycerol of the nodulin 26 subfamily of plant membrane intrinsic proteins.
2002,
Pubmed
Weig,
Functional identification of the glycerol permease activity of Arabidopsis thaliana NLM1 and NLM2 proteins by heterologous expression in Saccharomyces cerevisiae.
2000,
Pubmed
Wienkoop,
Proteome analysis. Novel proteins identified at the peribacteroid membrane from Lotus japonicus root nodules.
2003,
Pubmed
van Dongen,
Electrodiffusional uptake of organic cations by pea seed coats. Further evidence for poorly selective pores in the plasma membrane of seed coat parenchyma cells.
2001,
Pubmed
