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Transport and sorting of the solanum tuberosum sucrose transporter SUT1 is affected by posttranslational modification.
Krügel U
,
Veenhoff LM
,
Langbein J
,
Wiederhold E
,
Liesche J
,
Friedrich T
,
Grimm B
,
Martinoia E
,
Poolman B
,
Kühn C
.
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The plant sucrose transporter SUT1 from Solanum tuberosum revealed a dramatic redox-dependent increase in sucrose transport activity when heterologously expressed in Saccharomyces cerevisiae. Plant plasma membrane vesicles do not show any change in proton flux across the plasma membrane in the presence of redox reagents, indicating a SUT1-specific effect of redox reagents. Redox-dependent sucrose transport activity was confirmed electrophysiologically in Xenopus laevis oocytes with SUT1 from maize (Zea mays). Localization studies of green fluorescent protein fusion constructs showed that an oxidative environment increased the targeting of SUT1 to the plasma membrane where the protein concentrates in 200- to 300-nm raft-like microdomains. Using plant plasma membranes, St SUT1 can be detected in the detergent-resistant membrane fraction. Importantly, in yeast and in plants, oxidative reagents induced a shift in the monomer to dimer equilibrium of the St SUT1 protein and increased the fraction of dimer. Biochemical methods confirmed the capacity of SUT1 to form a dimer in plants and yeast cells in a redox-dependent manner. Blue native PAGE, chemical cross-linking, and immunoprecipitation, as well as the analysis of transgenic plants with reduced expression of St SUT1, confirmed the dimerization of St SUT1 and Sl SUT1 (from Solanum lycopersicum) in planta. The ability to form homodimers in plant cells was analyzed by the split yellow fluorescent protein technique in transiently transformed tobacco (Nicotiana tabacum) leaves and protoplasts. Oligomerization seems to be cell type specific since under native-like conditions, a phloem-specific reduction of the dimeric form of the St SUT1 protein was detectable in SUT1 antisense plants, whereas constitutively inhibited antisense plants showed reduction only of the monomeric form. The role of redox control of sucrose transport in plants is discussed.
Alberini,
Secretion of immunoglobulin M assembly intermediates in the presence of reducing agents.
1990, Pubmed
Alberini,
Secretion of immunoglobulin M assembly intermediates in the presence of reducing agents.
1990,
Pubmed
Alosi,
The regulation of gelation of Phloem exudate from cucurbita fruit by dilution, glutathione, and glutathione reductase.
1988,
Pubmed
Andrews,
Comparison of transient protein expression in tobacco leaves and plant suspension culture.
2005,
Pubmed
Balmer,
A complete ferredoxin/thioredoxin system regulates fundamental processes in amyloplasts.
2006,
Pubmed
Bolwell,
Pathogenic infection and the oxidative defences in plant apoplast.
2001,
Pubmed
Boorer,
Transport mechanism of the cloned potato H+/sucrose cotransporter StSUT1.
1996,
Pubmed
,
Xenbase
Borner,
Analysis of detergent-resistant membranes in Arabidopsis. Evidence for plasma membrane lipid rafts.
2005,
Pubmed
Bourquin,
Inhibition of Loading of C Assimilates by p-Chloromercuribenzenesulfonic Acid : Localization of the Apoplastic Pathway in Vicia faba.
1990,
Pubmed
Brown,
Functions of lipid rafts in biological membranes.
1998,
Pubmed
Bush,
Inhibitors of the proton-sucrose symport.
1993,
Pubmed
Bush,
Proton-Coupled Sucrose Transport in Plasmalemma Vesicles Isolated from Sugar Beet (Beta vulgaris L. cv Great Western) Leaves.
1989,
Pubmed
Carpaneto,
Phloem-localized, proton-coupled sucrose carrier ZmSUT1 mediates sucrose efflux under the control of the sucrose gradient and the proton motive force.
2005,
Pubmed
,
Xenbase
Chincinska,
Sucrose transporter StSUT4 from potato affects flowering, tuberization, and shade avoidance response.
2008,
Pubmed
Diaz-Vivancos,
The apoplastic antioxidant system in Prunus: response to long-term plum pox virus infection.
2006,
Pubmed
Flury,
An Inducible Glutathione S-Transferase in Soybean Hypocotyl Is Localized in the Apoplast.
1996,
Pubmed
Fra,
Quality control of ER synthesized proteins: an exposed thiol group as a three-way switch mediating assembly, retention and degradation.
1993,
Pubmed
Franceschi,
L-Ascorbic acid is accumulated in source leaf phloem and transported to sink tissues in plants.
2002,
Pubmed
Gao,
Distinct protein domains of the yeast Golgi GDP-mannose transporter mediate oligomer assembly and export from the endoplasmic reticulum.
2000,
Pubmed
Grossmann,
Lipid raft-based membrane compartmentation of a plant transport protein expressed in Saccharomyces cerevisiae.
2006,
Pubmed
Grossmann,
Membrane potential governs lateral segregation of plasma membrane proteins and lipids in yeast.
2007,
Pubmed
Gutscher,
Real-time imaging of the intracellular glutathione redox potential.
2008,
Pubmed
Hackel,
Sucrose transporter LeSUT1 and LeSUT2 inhibition affects tomato fruit development in different ways.
2006,
Pubmed
Hanzal-Bayer,
Lipid rafts and membrane traffic.
2007,
Pubmed
Hendriks,
ADP-glucose pyrophosphorylase is activated by posttranslational redox-modification in response to light and to sugars in leaves of Arabidopsis and other plant species.
2003,
Pubmed
Henriksen,
Identification of intra- and intermolecular disulfide bridges in the multidrug resistance transporter ABCG2.
2005,
Pubmed
Heuberger,
Oligomeric state of membrane transport proteins analyzed with blue native electrophoresis and analytical ultracentrifugation.
2002,
Pubmed
Hu,
Abscisic acid-induced apoplastic H2O2 accumulation up-regulates the activities of chloroplastic and cytosolic antioxidant enzymes in maize leaves.
2005,
Pubmed
Ikonen,
Roles of lipid rafts in membrane transport.
2001,
Pubmed
Ishiwatari,
Thioredoxin h is one of the major proteins in rice phloem sap.
1995,
Pubmed
Jamai,
Characterization of Glutathione Uptake in Broad Bean Leaf Protoplasts.
1996,
Pubmed
Khan,
The mechanism of inhibition of the Ca(2+)-ATPase of skeletal-muscle sarcoplasmic reticulum by the cross-linker o-phthalaldehyde.
1996,
Pubmed
Kolbe,
Combined transcript and metabolite profiling of Arabidopsis leaves reveals fundamental effects of the thiol-disulfide status on plant metabolism.
2006,
Pubmed
Kühn,
Macromolecular trafficking indicated by localization and turnover of sucrose transporters in enucleate sieve elements.
1997,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lajoie,
Regulation of raft-dependent endocytosis.
2007,
Pubmed
Lalonde,
Fusion to GFP blocks intercellular trafficking of the sucrose transporter SUT1 leading to accumulation in companion cells.
2003,
Pubmed
Laude,
Plasma membrane microdomains: organization, function and trafficking.
2004,
Pubmed
Laughery,
Mutational analysis of alpha-beta subunit interactions in the delivery of Na,K-ATPase heterodimers to the plasma membrane.
2003,
Pubmed
Lefebvre,
Characterization of lipid rafts from Medicago truncatula root plasma membranes: a proteomic study reveals the presence of a raft-associated redox system.
2007,
Pubmed
Lemaire,
Plant thioredoxin gene expression: control by light, circadian clock, and heavy metals.
2002,
Pubmed
Lemaire,
The complex regulation of ferredoxin/thioredoxin-related genes by light and the circadian clock.
1999,
Pubmed
Liang,
The C-terminus of prenylin is important in forming a dimer conformation necessary for endoplasmic-reticulum-to-Golgi transport.
2004,
Pubmed
Lichtner,
Sucrose uptake by developing soybean cotyledons.
1981,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
M'batchi,
Parachloromercuribenzenesulfonic Acid : a potential tool for differential labeling of the sucrose transporter.
1984,
Pubmed
Malínská,
Visualization of protein compartmentation within the plasma membrane of living yeast cells.
2003,
Pubmed
Marino,
Evidence for transcriptional and post-translational regulation of sucrose synthase in pea nodules by the cellular redox state.
2008,
Pubmed
Maurel,
Plant aquaporins: novel functions and regulation properties.
2007,
Pubmed
Maurousset,
Sulfur dioxide inhibits the sucrose carrier of the plant plasma membrane.
1992,
Pubmed
Mesecke,
A disulfide relay system in the intermembrane space of mitochondria that mediates protein import.
2005,
Pubmed
Mikosch,
Diacidic motif is required for efficient transport of the K+ channel KAT1 to the plasma membrane.
2006,
Pubmed
Mongrand,
Lipid rafts in higher plant cells: purification and characterization of Triton X-100-insoluble microdomains from tobacco plasma membrane.
2004,
Pubmed
Morel,
Proteomics of plant detergent-resistant membranes.
2006,
Pubmed
Murphy,
Endocytotic cycling of PM proteins.
2005,
Pubmed
Or,
Specific cross-links between fragments of proteolyzed Na,K-ATPase induced by o-phthalaldehyde.
1998,
Pubmed
Palmgren,
Lysophosphatidylcholine stimulates ATP dependent proton accumulation in isolated oat root plasma membrane vesicles.
1989,
Pubmed
Palmgren,
An H-ATPase Assay: Proton Pumping and ATPase Activity Determined Simultaneously in the Same Sample.
1990,
Pubmed
Parton,
Lipid rafts and caveolae as portals for endocytosis: new insights and common mechanisms.
2003,
Pubmed
Reinders,
Protein-protein interactions between sucrose transporters of different affinities colocalized in the same enucleate sieve element.
2002,
Pubmed
Riesmeier,
Potato sucrose transporter expression in minor veins indicates a role in phloem loading.
1993,
Pubmed
Riesmeier,
Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning.
1994,
Pubmed
Riesmeier,
Isolation and characterization of a sucrose carrier cDNA from spinach by functional expression in yeast.
1992,
Pubmed
Roblin,
Regulation of a plant plasma membrane sucrose transporter by phosphorylation.
1998,
Pubmed
Salahpour,
Homodimerization of the beta2-adrenergic receptor as a prerequisite for cell surface targeting.
2004,
Pubmed
Samaj,
Endocytosis, actin cytoskeleton, and signaling.
2004,
Pubmed
Schägger,
Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis.
1994,
Pubmed
Simons,
Functional rafts in cell membranes.
1997,
Pubmed
Szederkényi,
Cloning of the cDNA for glutaredoxin, an abundant sieve-tube exudate protein from Ricinus communis L. and characterisation of the glutathione-dependent thiol-reduction system in sieve tubes.
1997,
Pubmed
Thompson,
A plasma membrane-anchored fluorescent protein fusion illuminates sieve element plasma membranes in Arabidopsis and tobacco.
2008,
Pubmed
Vanacker,
Pathogen-induced changes in the antioxidant status of the apoplast in barley leaves.
1998,
Pubmed
Veenhoff,
Quaternary structure and function of transport proteins.
2002,
Pubmed
Walter,
Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation.
2004,
Pubmed
Walz,
Evidence for the presence and activity of a complete antioxidant defence system in mature sieve tubes.
2002,
Pubmed
Wang,
Identification and characterization of a phloem-specific beta-amylase.
1995,
Pubmed
Weise,
Introns control expression of sucrose transporter LeSUT1 in trichomes, companion cells and in guard cells.
2008,
Pubmed
Weise,
A new subfamily of sucrose transporters, SUT4, with low affinity/high capacity localized in enucleate sieve elements of plants.
2000,
Pubmed