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Chromatin-mediated restriction of nuclear factor 1/CTF binding in a repressed and hormone-activated promoter in vivo.
Belikov S
,
Astrand C
,
Holmqvist PH
,
Wrange O
.
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Mouse mammary tumor virus (MMTV) promoter-driven transcription is induced by glucocorticoid hormone via binding of the glucocorticoid receptor (GR). The MMTV promoter also harbors a binding site for nuclear factor 1 (NF1). NF1 and GR were expressed in Xenopus oocytes; this revealed GR-NF1 cooperativity both in terms of DNA binding and chromatin remodeling but not transcription. A fraction of NF1 sites were occupied in a hormone-dependent fashion, but a significant and NF1 concentration-dependent fraction were constitutively bound. Activation of the MMTV promoter resulted in an approximately 50-fold increase in the NF1 accessibility for its DNA site. The hormone-dependent component of NF1 binding was dissociated by addition of a GR antagonist; however, the antagonist RU486, which supports partial GR-DNA binding, also maintained partial NF1 binding. Hence GR-NF1 cooperativity is independent of agonist-driven chromatin remodeling. NF1 induced the formation of a micrococcal-nuclease-resistant protein-DNA complex containing the DNA segment from -185 to -55, the MMTV enhanceosome. Coexpression of NF1 and Oct1 resulted in a significant stimulation of hormone-induced MMTV transcription and also in increased basal transcription. We propose that hormone-independent NF1 binding may be involved in maintaining transcriptional competence and establishment of tissue-specific gene networks.
Adams,
Binding of disparate transcriptional activators to nucleosomal DNA is inherently cooperative.
1995, Pubmed
Adams,
Binding of disparate transcriptional activators to nucleosomal DNA is inherently cooperative.
1995,
Pubmed
Almouzni,
Replication-coupled chromatin assembly is required for the repression of basal transcription in vivo.
1993,
Pubmed
,
Xenbase
Anderson,
Spontaneous access of proteins to buried nucleosomal DNA target sites occurs via a mechanism that is distinct from nucleosome translocation.
2002,
Pubmed
Archer,
Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter.
1991,
Pubmed
Belikov,
Hormone-induced nucleosome positioning in the MMTV promoter is reversible.
2001,
Pubmed
,
Xenbase
Belikov,
Hormone activation induces nucleosome positioning in vivo.
2000,
Pubmed
,
Xenbase
Blomquist,
The affinity of nuclear factor 1 for its DNA site is drastically reduced by nucleosome organization irrespective of its rotational or translational position.
1996,
Pubmed
Blomquist,
Increased nuclear factor 1 binding to its nucleosomal site mediated by sequence-dependent DNA structure.
1999,
Pubmed
Brüggemeier,
Ubiquitous transcription factor OTF-1 mediates induction of the MMTV promoter through synergistic interaction with hormone receptors.
1991,
Pubmed
Buetti,
Dual function of a nuclear factor I binding site in MMTV transcription regulation.
1989,
Pubmed
Buetti,
Stably integrated mouse mammary tumor virus long terminal repeat DNA requires the octamer motifs for basal promoter activity.
1994,
Pubmed
Buetti,
Distinct sequence elements involved in the glucocorticoid regulation of the mouse mammary tumor virus promoter identified by linker scanning mutagenesis.
1986,
Pubmed
Cato,
DNA sequences outside the receptor-binding sites differently modulate the responsiveness of the mouse mammary tumour virus promoter to various steroid hormones.
1988,
Pubmed
Chaudhry,
Nuclear factor I (NFI) isoforms differentially activate simple versus complex NFI-responsive promoters.
1998,
Pubmed
Cordingley,
Steroid-dependent interaction of transcription factors with the inducible promoter of mouse mammary tumor virus in vivo.
1987,
Pubmed
Di Croce,
Two-step synergism between the progesterone receptor and the DNA-binding domain of nuclear factor 1 on MMTV minichromosomes.
1999,
Pubmed
Eisfeld,
Binding of NF1 to the MMTV promoter in nucleosomes: influence of rotational phasing, translational positioning and histone H1.
1997,
Pubmed
Gelius,
Glucocorticoid hormone-induced receptor localization to the chromatin fibers formed on injected DNA in Xenopus oocytes.
2001,
Pubmed
,
Xenbase
Gelius,
Characterization of a chromatin remodelling activity in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Gronostajski,
Roles of the NFI/CTF gene family in transcription and development.
2000,
Pubmed
Gründer,
Nuclear factor I-B (Nfib) deficient mice have severe lung hypoplasia.
2002,
Pubmed
Han,
Depletion of histone H4 and nucleosomes activates the PHO5 gene in Saccharomyces cerevisiae.
1988,
Pubmed
Kim,
Oct-1 protein promotes functional transcription complex assembly on the mouse mammary tumor virus promoter.
1995,
Pubmed
Kruse,
Transcription factor nuclear factor I proteins form stable homo- and heterodimers.
1994,
Pubmed
Kutoh,
Functional interference between the ubiquitous and constitutive octamer transcription factor 1 (OTF-1) and the glucocorticoid receptor by direct protein-protein interaction involving the homeo subdomain of OTF-1.
1992,
Pubmed
Li,
Accessibility of a glucocorticoid response element in a nucleosome depends on its rotational positioning.
1995,
Pubmed
Luisi,
Crystallographic analysis of the interaction of the glucocorticoid receptor with DNA.
1991,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
McNally,
The glucocorticoid receptor: rapid exchange with regulatory sites in living cells.
2000,
Pubmed
Mermod,
The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain.
1989,
Pubmed
Nagata,
Adenovirus DNA replication in vitro: identification of a host factor that stimulates synthesis of the preterminal protein-dCMP complex.
1982,
Pubmed
Nowock,
The TGGCA protein binds to the MMTV-LTR, the adenovirus origin of replication, and the BK virus enhancer.
1985,
Pubmed
Payvar,
Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment whose transcription is regulated by glucocorticoids in vivo.
1981,
Pubmed
Perlmann,
Specific glucocorticoid receptor binding to DNA reconstituted in a nucleosome.
1988,
Pubmed
Phillips,
The virtuoso of versatility: POU proteins that flex to fit.
2000,
Pubmed
Piña,
Nucleosome positioning modulates accessibility of regulatory proteins to the mouse mammary tumor virus promoter.
1990,
Pubmed
Prado,
Accurate chromatin organization of the mouse mammary tumor virus promoter determines the nature of the synergism between transcription factors.
2002,
Pubmed
Prado,
Differential role of the proline-rich domain of nuclear factor 1-C splice variants in DNA binding and transactivation.
2002,
Pubmed
Préfontaine,
Recruitment of octamer transcription factors to DNA by glucocorticoid receptor.
1998,
Pubmed
Richard-Foy,
Sequence-specific positioning of nucleosomes over the steroid-inducible MMTV promoter.
1987,
Pubmed
Ringold,
Dexamethasone-mediated induction of mouse mammary tumor virus RNA: a system for studying glucocorticoid action.
1975,
Pubmed
Roulet,
Experimental analysis and computer prediction of CTF/NFI transcription factor DNA binding sites.
2000,
Pubmed
Spangenberg,
The mouse mammary tumour virus promoter positioned on a tetramer of histones H3 and H4 binds nuclear factor 1 and OTF1.
1998,
Pubmed
Steele-Perkins,
Essential role for NFI-C/CTF transcription-replication factor in tooth root development.
2003,
Pubmed
Toohey,
Functional elements of the steroid hormone-responsive promoter of mouse mammary tumor virus.
1990,
Pubmed
Truss,
Hormone induces binding of receptors and transcription factors to a rearranged nucleosome on the MMTV promoter in vivo.
1995,
Pubmed
Zaret,
Reversible and persistent changes in chromatin structure accompany activation of a glucocorticoid-dependent enhancer element.
1984,
Pubmed
Zernicka-Goetz,
An indelible lineage marker for Xenopus using a mutated green fluorescent protein.
1996,
Pubmed
,
Xenbase
das Neves,
Disruption of the murine nuclear factor I-A gene (Nfia) results in perinatal lethality, hydrocephalus, and agenesis of the corpus callosum.
1999,
Pubmed
