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Tubulin as a binding partner of the heag2 voltage-gated potassium channel.
Bracey K
,
Ju M
,
Tian C
,
Stevens L
,
Wray D
.
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The aim of this work was to investigate interactions of the human ether-a-go-go channel heag2 with human brain proteins. For this, we used heag2-GST fusion proteins in pull-down assays with brain proteins and mass spectrometry, as well as coimmunoprecipitation. We identified tubulin and heat shock 70 proteins as binding to intracellular C-terminal regions of the channel. To study functional effects, heag2 channels were expressed in Xenopus laevis oocytes for two-electrode voltage clamping. Coexpression of alpha-tubulin or the application of colchicine significantly prolonged channel activation times. Application at different times of colchicine gave similar results. The data suggest that colchicine application and tubulin expression do not affect heag2 trafficking and that tubulin may associate with the channel to cause functional effects. Coexpression of heat shock 70 proteins had no functional effect on the channel. The role of tubulin in the cell cytoskeleton suggests a link for the heag2 channel in tubulin-dependent physiological functions, such as cellular proliferation.
Bauer,
Physiology of EAG K+ channels.
2001, Pubmed
Bauer,
Physiology of EAG K+ channels.
2001,
Pubmed
Bianchi,
herg encodes a K+ current highly conserved in tumors of different histogenesis: a selective advantage for cancer cells?
1998,
Pubmed
,
Xenbase
Brüggemann,
Mitosis-promoting factor-mediated suppression of a cloned delayed rectifier potassium channel expressed in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Camacho,
Cytoskeletal interactions determine the electrophysiological properties of human EAG potassium channels.
2000,
Pubmed
Camacho,
Ether à go-go potassium channels and cancer.
2006,
Pubmed
Chappell,
Uncoating ATPase is a member of the 70 kilodalton family of stress proteins.
1986,
Pubmed
Chen,
The gamma-aminobutyric acid type A (GABAA) receptor-associated protein (GABARAP) promotes GABAA receptor clustering and modulates the channel kinetics.
2000,
Pubmed
Chirico,
70K heat shock related proteins stimulate protein translocation into microsomes.
1988,
Pubmed
Farias,
Ether a go-go potassium channels as human cervical cancer markers.
2004,
Pubmed
Ficker,
Role of the cytosolic chaperones Hsp70 and Hsp90 in maturation of the cardiac potassium channel HERG.
2003,
Pubmed
Galli,
Inactivation of L-type Ca channels in embryonic chick ventricle cells: dependence on the cytoskeletal agents colchicine and taxol.
1994,
Pubmed
Givogri,
New insights on the biology of myelin basic protein gene: the neural-immune connection.
2000,
Pubmed
Ju,
The Roles of N- and C-terminal determinants in the activation of the Kv2.1 potassium channel.
2003,
Pubmed
,
Xenbase
Ju,
Molecular identification and characterisation of the human eag2 potassium channel.
2002,
Pubmed
Ju,
Molecular regions responsible for differences in activation between heag channels.
2006,
Pubmed
,
Xenbase
Kirsch,
The 93-kDa glycine receptor-associated protein binds to tubulin.
1991,
Pubmed
Lang,
Ion channels in cell proliferation and apoptotic cell death.
2005,
Pubmed
Massover,
Complex surface invaginations in frog oocytes.
1973,
Pubmed
Mello de Queiroz,
Ether à go-go potassium channel expression in soft tissue sarcoma patients.
2006,
Pubmed
Modesti,
The interaction of myelin basic protein with tubulin and the inhibition of tubulin carboxypeptidase activity.
1986,
Pubmed
Moran,
Functional properties of sodium channels do not depend on the cytoskeleton integrity.
2000,
Pubmed
,
Xenbase
Nogales,
Structure of the alpha beta tubulin dimer by electron crystallography.
1998,
Pubmed
Pardo,
Oncogenic potential of EAG K(+) channels.
1999,
Pubmed
Pardo,
Voltage-gated potassium channels in cell proliferation.
2004,
Pubmed
Pardo,
Cell cycle-related changes in the conducting properties of r-eag K+ channels.
1998,
Pubmed
,
Xenbase
Patel,
The 2P-domain K+ channels: role in apoptosis and tumorigenesis.
2004,
Pubmed
Perkins,
Probability-based protein identification by searching sequence databases using mass spectrometry data.
1999,
Pubmed
Pestell,
Microtubule protein synthesis during oogenesis and early embryogenesis in Xenopus laevis.
1975,
Pubmed
,
Xenbase
Sackett,
Molecular mechanism of colchicine action: induced local unfolding of beta-tubulin.
1993,
Pubmed
Schlossman,
An enzyme that removes clathrin coats: purification of an uncoating ATPase.
1984,
Pubmed
Schönherr,
Functional distinction of human EAG1 and EAG2 potassium channels.
2002,
Pubmed
,
Xenbase
Toral,
Effect of extracellular matrix on adhesion, viability, actin cytoskeleton and K+ currents of cells expressing human ether à go-go channels.
2007,
Pubmed
Torin Huzil,
Comparative modelling of human β tubulin isotypes and implications for drug binding.
2006,
Pubmed
Venter,
Molecular dissection of membrane-transport proteins: mass spectrometry and sequence determination of the galactose-H+ symport protein, GalP, of Escherichia coli and quantitative assay of the incorporation of [ring-2-13C]histidine and (15)NH(3).
2002,
Pubmed
Wang,
Roles of K+ channels in regulating tumour cell proliferation and apoptosis.
2004,
Pubmed
Wang,
GABA(A)-receptor-associated protein links GABA(A) receptors and the cytoskeleton.
1999,
Pubmed
Wilson,
Role for the oligodendrocyte cytoskeleton in myelination.
1989,
Pubmed
Yellen,
The voltage-gated potassium channels and their relatives.
2002,
Pubmed
Zagotta,
Structural basis for modulation and agonist specificity of HCN pacemaker channels.
2003,
Pubmed
van Rossum,
Dynamic interaction between soluble tubulin and C-terminal domains of N-methyl-D-aspartate receptor subunits.
1999,
Pubmed
