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Medium weight neurofilament mRNA in goldfish Mauthner axoplasm.
Weiner OD
,
Zorn AM
,
Krieg PA
,
Bittner GD
.
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Although axons are generally considered to lack the ability to synthesize proteins, the Mauthner axon (M-axon) of the goldfish has been reported to contain some of the basic components of the translational machinery, such as transfer RNA (tRNA), ribosomal RNA (rRNA), and ribosomes. To determine if the M-axon also contains mRNA, we isolated samples of M-axoplasm free of glial contamination as demonstrated by the absence of glial-specific mRNA and protein. Reverse transcription-polymerase chain reaction (RT-PCR) of M-axoplasmic cDNA in the presence of primers for the goldfish medium-weight neurofilament (NF-M) gene produced a single product of the expected length for RT-PCR amplification of goldfish NF-M mRNA. This mRNA might direct protein synthesis of NF-M within the M-axoplasm.
Balcarek,
Structure of the mouse glial fibrillary acidic protein gene: implications for the evolution of the intermediate filament multigene family.
1985, Pubmed
Balcarek,
Structure of the mouse glial fibrillary acidic protein gene: implications for the evolution of the intermediate filament multigene family.
1985,
Pubmed
Bittner,
Long-term survival of anucleate axons and its implications for nerve regeneration.
1991,
Pubmed
Black,
Slow components of axonal transport: two cytoskeletal networks.
1980,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Dirks,
Ultrastructural evidence for the axonal localization of caudodorsal cell hormone mRNA in the central nervous system of the mollusc Lymnaea stagnalis.
1993,
Pubmed
Gioio,
Kinesin mRNA is present in the squid giant axon.
1994,
Pubmed
Giuditta,
Active polysomes in the axoplasm of the squid giant axon.
1991,
Pubmed
Glasgow,
Organization, sequence, and expression of a gene encoding goldfish neurofilament medium protein.
1994,
Pubmed
Jirikowski,
mRNA coding for oxytocin is present in axons of the hypothalamo-neurohypophysial tract.
1990,
Pubmed
Kislauskis,
Sequences responsible for intracellular localization of beta-actin messenger RNA also affect cell phenotype.
1994,
Pubmed
Kloppstech,
Stability of poly(A)(+)RNA in nucleate and anucleate cells of Acetabularia.
1982,
Pubmed
Koenig,
Ribosomal RNA in Mauthner axon: implications for a protein synthesizing machinery in the myelinated axon.
1979,
Pubmed
Koenig,
Evaluation of local synthesis of axonal proteins in the goldfish Mauthner cell axon and axons of dorsal and ventral roots of the rat in vitro.
1991,
Pubmed
Koenig,
Cortical plaque-like structures identify ribosome-containing domains in the Mauthner cell axon.
1996,
Pubmed
Lasek,
Cell-to-cell transfer of glial proteins to the squid giant axon. The glia-neuron protein trnasfer hypothesis.
1977,
Pubmed
Levy,
Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein.
1987,
Pubmed
Moehlenbruck,
Long-term survival followed by degradation of neurofilament proteins in severed mauthner axons of goldfish.
1994,
Pubmed
Mohr,
Differential subcellular mRNA targeting: deletion of a single nucleotide prevents the transport to axons but not to dendrites of rat hypothalamic magnocellular neurons.
1995,
Pubmed
Nixon,
Protein degradation in the mouse visual system. I. Degradation of axonally transported and retinal proteins.
1980,
Pubmed
Skutella,
Localization of tyrosine hydroxylase mRNA in the axons of the hypothalamo-neurohypophysial system.
1994,
Pubmed
Tanner,
Maintenance and degradation of proteins in intact and severed axons: implications for the mechanisms of long-term survival of anucleate crayfish axons.
1995,
Pubmed
