Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Comp Physiol B
2008 May 01;1784:457-63. doi: 10.1007/s00360-007-0238-4.
Show Gene links
Show Anatomy links
Endogenous lactate transport in Xenopus laevis oocyte: dependence on cytoskeleton and regulation by protein kinases.
Tosco M
,
Faelli A
,
Gastaldi G
,
Paulmichl M
,
Orsenigo MN
.
???displayArticle.abstract???
Carbon flux in Xenopus laevis oocyte is glycogenic and an endogenous monocarboxylate transporter is responsible for intracellular lactate uptake. The aim of the present study was to determine if direct activation of protein kinases C and A modulates the activity of lactate transporter, as well as to investigate the possible role of cytoskeleton in these regulatory phenomena. The modulation was studied in isolated Xenopus oocytes of stage V-VI by measuring (14)C-lactate uptake, both in the absence and in the presence of cytoskeletal-perturbing toxins. We found that the basal lactate transporter activity depends on the integrity of the cytoskeleton since it is partially inhibited by cytoskeleton disorganisation. Both PKA and PKC activation caused a significant decrease in transport activity and this decrease could be blocked by specific protein kinase inhibitors. The evidenced effects were not additive. Transport inhibition was annulled by agents that destabilize actin filaments or microtubules. We conclude that both protein kinases A and C, whose effects are mediated by cytoskeleton, negatively regulate the endogenous lactate transporter of Xenopus oocyte, suggesting that these kinases may have a role in the control of cytosolic pyruvate/lactate pool in the oocyte.
Bement,
Activators of protein kinase C trigger cortical granule exocytosis, cortical contraction, and cleavage furrow formation in Xenopus laevis oocytes and eggs.
1989, Pubmed,
Xenbase
Bement,
Activators of protein kinase C trigger cortical granule exocytosis, cortical contraction, and cleavage furrow formation in Xenopus laevis oocytes and eggs.
1989,
Pubmed
,
Xenbase
Coady,
The human tumour suppressor gene SLC5A8 expresses a Na+-monocarboxylate cotransporter.
2004,
Pubmed
,
Xenbase
Dekel,
Cellular, biochemical and molecular mechanisms regulating oocyte maturation.
2005,
Pubmed
Dworkin,
Regulation of carbon flux from amino acids into sugar phosphates in Xenopus embryos.
1990,
Pubmed
,
Xenbase
Dworkin,
Metabolic regulation during early frog development: glycogenic flux in Xenopus oocytes, eggs, and embryos.
1989,
Pubmed
,
Xenbase
Dworkin,
Carbon metabolism in early amphibian embryos.
1991,
Pubmed
,
Xenbase
Forster,
Protein kinase C activators induce membrane retrieval of type II Na+-phosphate cotransporters expressed in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Frank-Vaillant,
Progesterone regulates the accumulation and the activation of Eg2 kinase in Xenopus oocytes.
2000,
Pubmed
,
Xenbase
Gupta,
SLC5A8 (SMCT1)-mediated transport of butyrate forms the basis for the tumor suppressive function of the transporter.
2006,
Pubmed
Halestrap,
The SLC16 gene family-from monocarboxylate transporters (MCTs) to aromatic amino acid transporters and beyond.
2004,
Pubmed
Hirsch,
Regulation of Na+/glucose cotransporter expression by protein kinases in Xenopus laevis oocytes.
1996,
Pubmed
,
Xenbase
Hoffmann,
The role of volume-sensitive ion transport systems in regulation of epithelial transport.
2007,
Pubmed
Kim,
Modulation of lysophosphatidic acid-induced Cl- currents by protein kinases A and C in the Xenopus oocyte.
2000,
Pubmed
,
Xenbase
Kurashima,
The apical Na(+)/H(+) exchanger isoform NHE3 is regulated by the actin cytoskeleton.
1999,
Pubmed
Liao,
Insulin modulation of cloned mouse NMDA receptor currents in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Lo,
An endogenous carrier-mediated uptake system for folate in oocytes of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Plata,
Protein kinase C activation reduces the function of the Na(+):K(+):2Cl(-) cotransporter in Xenopus laevis oocytes.
2000,
Pubmed
,
Xenbase
Said,
Uptake of biotin by native Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Sakai,
Involvement of the actin cytoskeleton in the regulation of serotonin transporter (SET) activity: possible mechanism underlying SET regulation by protein kinase C.
2000,
Pubmed
Srinivas,
Cloning and functional identification of slc5a12 as a sodium-coupled low-affinity transporter for monocarboxylates (SMCT2).
2005,
Pubmed
,
Xenbase
Tosco,
An endogenous monocarboxylate transport in Xenopus laevis oocytes.
2000,
Pubmed
,
Xenbase
Trotti,
Inhibition of the glutamate transporter EAAC1 expressed in Xenopus oocytes by phorbol esters.
2001,
Pubmed
,
Xenbase
Wang,
Progesterone inhibits protein kinase A (PKA) in Xenopus oocytes: demonstration of endogenous PKA activities using an expressed substrate.
2004,
Pubmed
,
Xenbase
Weber,
Endogenous D-glucose transport in oocytes of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Wolff,
Protein kinase C activation downregulates human organic anion transporter 1-mediated transport through carrier internalization.
2003,
Pubmed
,
Xenbase
