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Probing the interaction between KCNE2 and KCNQ1 in their transmembrane regions.
Liu XS
,
Zhang M
,
Jiang M
,
Wu DM
,
Tseng GN
.
???displayArticle.abstract??? KCNE1-KCNE5 are single membrane-spanning proteins that associate with voltage-gated potassium channels to diversify their function. Other than the KCNQ1/KCNE1 complex, little is known about how KCNE proteins work. We focus on KCNE2, which associates with KCNQ1 to form K channels critical for gastric acid secretion in parietal cells. We use cysteine (Cys)-scanning mutagenesis to probe the functional role of residues along the KCNE2 transmembrane domain (TMD) in modulating KCNQ1 function. There is an alpha-helical periodicity in how Cys substitutions along the KCNE2TMD perturb KCNQ1 pore conductance/ion selectivity. However, positions where Cys substitutions perturb KCNQ1 gating kinetics cluster to the extracellular end and cytoplasmic half of the KCNE2TMD. This is the first systematic perturbation analysis of a KCNE TMD. We propose that the KCNE2TMD adopts an alpha-helical secondary structure with one face making intimate contact with the KCNQ1 pore domain, while the contacts with the KCNQ1 voltage-sensing domain appear more dynamic.
Abbott,
A superfamily of small potassium channel subunits: form and function of the MinK-related peptides (MiRPs).
1998, Pubmed
Abbott,
A superfamily of small potassium channel subunits: form and function of the MinK-related peptides (MiRPs).
1998,
Pubmed
Abbott,
Disease-associated mutations in KCNE potassium channel subunits (MiRPs) reveal promiscuous disruption of multiple currents and conservation of mechanism.
2002,
Pubmed
Aggeli,
Conformation and ion-channeling activity of a 27-residue peptide modeled on the single-transmembrane segment of the IsK (minK) protein.
1998,
Pubmed
Bendahhou,
In vitro molecular interactions and distribution of KCNE family with KCNQ1 in the human heart.
2005,
Pubmed
Chandrasekhar,
KCNE1 subunits require co-assembly with K+ channels for efficient trafficking and cell surface expression.
2006,
Pubmed
Chen,
KCNQ1 gain-of-function mutation in familial atrial fibrillation.
2003,
Pubmed
Franqueza,
Long QT syndrome-associated mutations in the S4-S5 linker of KvLQT1 potassium channels modify gating and interaction with minK subunits.
1999,
Pubmed
,
Xenbase
Goldstein,
Site-specific mutations in a minimal voltage-dependent K+ channel alter ion selectivity and open-channel block.
1991,
Pubmed
,
Xenbase
Hong,
De novo KCNQ1 mutation responsible for atrial fibrillation and short QT syndrome in utero.
2005,
Pubmed
,
Xenbase
Isbrandt,
Identification and functional characterization of a novel KCNE2 (MiRP1) mutation that alters HERG channel kinetics.
2002,
Pubmed
LeMasurier,
KcsA: it's a potassium channel.
2001,
Pubmed
Lerche,
Molecular impact of MinK on the enantiospecific block of I(Ks) by chromanols.
2000,
Pubmed
,
Xenbase
Liu,
Structural and functional role of the extracellular s5-p linker in the HERG potassium channel.
2002,
Pubmed
,
Xenbase
Long,
Voltage sensor of Kv1.2: structural basis of electromechanical coupling.
2005,
Pubmed
Lu,
Coupling between voltage sensors and activation gate in voltage-gated K+ channels.
2002,
Pubmed
,
Xenbase
Melman,
Structural determinants of KvLQT1 control by the KCNE family of proteins.
2001,
Pubmed
Melman,
A single transmembrane site in the KCNE-encoded proteins controls the specificity of KvLQT1 channel gating.
2002,
Pubmed
Morais-Cabral,
Energetic optimization of ion conduction rate by the K+ selectivity filter.
2001,
Pubmed
Popot,
Helical membrane protein folding, stability, and evolution.
2000,
Pubmed
Roepke,
The KCNE2 potassium channel ancillary subunit is essential for gastric acid secretion.
2006,
Pubmed
Sanguinetti,
Coassembly of K(V)LQT1 and minK (IsK) proteins to form cardiac I(Ks) potassium channel.
1996,
Pubmed
,
Xenbase
Schreibmayer,
Voltage clamping of Xenopus laevis oocytes utilizing agarose-cushion electrodes.
1994,
Pubmed
,
Xenbase
Sesti,
Single-channel characteristics of wild-type IKs channels and channels formed with two minK mutants that cause long QT syndrome.
1998,
Pubmed
,
Xenbase
Tai,
The conduction pore of a cardiac potassium channel.
1998,
Pubmed
,
Xenbase
Takumi,
Alteration of channel activities and gating by mutations of slow ISK potassium channel.
1991,
Pubmed
,
Xenbase
Tapper,
Location and orientation of minK within the I(Ks) potassium channel complex.
2001,
Pubmed
,
Xenbase
Tapper,
MinK subdomains that mediate modulation of and association with KvLQT1.
2000,
Pubmed
,
Xenbase
Teng,
Novel gene hKCNE4 slows the activation of the KCNQ1 channel.
2003,
Pubmed
,
Xenbase
Wang,
MinK residues line a potassium channel pore.
1996,
Pubmed
Wilson,
Changes in activation gating of IsK potassium currents brought about by mutations in the transmembrane sequence.
1994,
Pubmed
,
Xenbase
Wu,
KCNE2 is colocalized with KCNQ1 and KCNE1 in cardiac myocytes and may function as a negative modulator of I(Ks) current amplitude in the heart.
2006,
Pubmed
,
Xenbase
Wu,
Characterization of an LQT5-related mutation in KCNE1, Y81C: implications for a role of KCNE1 cytoplasmic domain in IKs channel function.
2006,
Pubmed
Zhang,
minK-related peptide 1 associates with Kv4.2 and modulates its gating function: potential role as beta subunit of cardiac transient outward channel?
2001,
Pubmed
,
Xenbase
Zhang,
Interactions between charged residues in the transmembrane segments of the voltage-sensing domain in the hERG channel.
2005,
Pubmed
,
Xenbase
