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Exp Eye Res
2008 Jan 01;861:81-91. doi: 10.1016/j.exer.2007.09.011.
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Expression of Kir7.1 and a novel Kir7.1 splice variant in native human retinal pigment epithelium.
Yang D
,
Swaminathan A
,
Zhang X
,
Hughes BA
.
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Previous studies on bovine retinal pigment epithelium (RPE) established that Kir7.1 channels compose this epithelium's large apical membrane K+ conductance. The purpose of this study was to determine whether Kir7.1 and potential Kir7.1 splice variants are expressed in native adult human RPE and, if so, to determine their function and how they are generated. RT-PCR analysis indicated that human RPE expresses full-length Kir7.1 and a novel Kir7.1 splice variant, designated Kir7.1S. Analysis of the human Kir7.1 gene (KCNJ13) organization revealed that it contains three exons, two introns, and a novel alternative 5' splice site in exon 2. In human RPE, the alternative usage of two competing 5' splice sites in exon 2 gives rise to transcripts encoding full-length Kir7.1 and Kir7.1S, which is predicted to encode a truncated protein. Real-time PCR indicated that Kir7.1 transcript is nearly as abundant as GAPDH mRNA in human RPE whereas Kir7.1S transcript expression is 4-fold lower. Western blot analysis showed that the splice variant is translated in Xenopus oocytes injected with Kir7.1S cRNA and revealed the expression of full-length Kir7.1 but not Kir7.1S in adult human RPE. Co-expression of Kir7.1 with Kir7.1S in Xenopus oocytes had no effect on either the kinetics or amplitude of Kir7.1 currents. This study confirms the expression of Kir7.1 in human RPE, identifies a Kir7.1 splice variant resulting in predicted changes in protein sequence, and indicates that there is no functional interaction between this splice variant and full-length Kir7.1.
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Buraczynska,
Gene expression profile of native human retinal pigment epithelium.
2002, Pubmed
Buraczynska,
Gene expression profile of native human retinal pigment epithelium.
2002,
Pubmed
Derst,
Partial gene structure and assignment to chromosome 2q37 of the human inwardly rectifying K+ channel (Kir7.1) gene (KCNJ13).
1998,
Pubmed
Döring,
The epithelial inward rectifier channel Kir7.1 displays unusual K+ permeation properties.
1998,
Pubmed
,
Xenbase
Geering,
A role for the beta-subunit in the expression of functional Na+-K+-ATPase in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Hughes,
Voltage-dependent currents in isolated cells of the frog retinal pigment epithelium.
1990,
Pubmed
Hughes,
Effects of Ba2+ and Cs+ on apical membrane K+ conductance in toad retinal pigment epithelium.
1995,
Pubmed
Hughes,
Inwardly rectifying K+ currents in isolated human retinal pigment epithelial cells.
1996,
Pubmed
Hughes,
ATP-dependent regulation of inwardly rectifying K+ current in bovine retinal pigment epithelial cells.
1998,
Pubmed
Isomoto,
Inwardly rectifying potassium channels: their molecular heterogeneity and function.
1997,
Pubmed
,
Xenbase
Kusaka,
Functional Kir7.1 channels localized at the root of apical processes in rat retinal pigment epithelium.
2001,
Pubmed
Nakamura,
Inwardly rectifying K+ channel Kir7.1 is highly expressed in thyroid follicular cells, intestinal epithelial cells and choroid plexus epithelial cells: implication for a functional coupling with Na+,K+-ATPase.
1999,
Pubmed
Nakamura,
Complex structure and regulation of expression of the rat gene for inward rectifier potassium channel Kir7.1.
2000,
Pubmed
Ookata,
Localization of inward rectifier potassium channel Kir7.1 in the basolateral membrane of distal nephron and collecting duct.
2000,
Pubmed
Shimura,
Expression and permeation properties of the K(+) channel Kir7.1 in the retinal pigment epithelium.
2001,
Pubmed
,
Xenbase
Tian,
Regulation of TRPV1 by a novel renally expressed rat TRPV1 splice variant.
2006,
Pubmed
Wischmeyer,
Stable cation coordination at a single outer pore residue defines permeation properties in Kir channels.
2000,
Pubmed
,
Xenbase
Yamada,
A truncated splice variant of KCNQ1 cloned from rat heart.
2002,
Pubmed
,
Xenbase
Yang,
Molecular cloning and expression of an inwardly rectifying K(+) channel from bovine corneal endothelial cells.
2000,
Pubmed
,
Xenbase
Yang,
Expression and localization of the inwardly rectifying potassium channel Kir7.1 in native bovine retinal pigment epithelium.
2003,
Pubmed
Yuan,
Regulation of inwardly rectifying K+ channels in retinal pigment epithelial cells by intracellular pH.
2003,
Pubmed
Zhang,
Effects of phospholipids on the oligomeric state of phospholamban of the cardiac sarcoplasmic reticulum.
2005,
Pubmed