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Planta
2007 Dec 01;2271:47-56. doi: 10.1007/s00425-007-0593-0.
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Foliar trichome- and aquaporin-aided water uptake in a drought-resistant epiphyte Tillandsia ionantha Planchon.
Ohrui T
,
Nobira H
,
Sakata Y
,
Taji T
,
Yamamoto C
,
Nishida K
,
Yamakawa T
,
Sasuga Y
,
Yaguchi Y
,
Takenaga H
,
Tanaka S
.
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The atmospheric epiphyte Tillandsia ionantha is capable of surviving drought stress for 6 months or more without any exogenous water supply via an as of yet to be determined mechanism. When plants were soaked in water for 3 h, leaves absorbed a remarkably large amount of water (30-40% on the basis of fresh weight), exhibiting a bimodal absorption pattern. Radiolabeled water was taken up by the leaves by capillary action of the epidermal trichomes within 1 min (phase 1) and then transported intracellularly to leaf tissues over 3 h (phase 2). The removal of epidermal trichome wings from leaves as well as rinsing leaves with water significantly lowered the extracellular accumulation of water on leaf surfaces. The intracellular transport of water was inhibited by mercuric chloride, implicating the involvement of a water channel aquaporin in second-phase water absorption. Four cDNA clones (TiPIP1a, TiPIP1b, TiPIP1c, and TiPIP2a) homologous to PIP family aquaporins were isolated from the leaves, and RT-PCR showed that soaking plants in water stimulated the expression of TiPIP2a mRNA, suggesting the reinforcement in ability to rapidly absorb a large amount of water. The expression of TiPIP2a complementary RNA in Xenopus oocytes enhanced permeability, and treatment with inhibitors suggested that the water channel activity of TiPIP2a protein was regulated by phosphorylation. Thus, the high water uptake capability of T. ionantha leaves surviving drought is attributable to a bimodal trichome- and aquaporin-aided water uptake system based on rapid physical collection of water and subsequent, sustained chemical absorption.
Aharon,
Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress.
2003, Pubmed
Aharon,
Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress.
2003,
Pubmed
Chaumont,
Regulation of plant aquaporin activity.
2005,
Pubmed
Cohen,
The structure and regulation of protein phosphatases.
1989,
Pubmed
Guenther,
Phosphorylation of soybean nodulin 26 on serine 262 enhances water permeability and is regulated developmentally and by osmotic signals.
2003,
Pubmed
,
Xenbase
Javot,
Role of a single aquaporin isoform in root water uptake.
2003,
Pubmed
Johansson,
The role of aquaporins in cellular and whole plant water balance.
2000,
Pubmed
,
Xenbase
Johansson,
Water transport activity of the plasma membrane aquaporin PM28A is regulated by phosphorylation.
1998,
Pubmed
,
Xenbase
Johansson,
The major integral proteins of spinach leaf plasma membranes are putative aquaporins and are phosphorylated in response to Ca2+ and apoplastic water potential.
1996,
Pubmed
Kaldenhoff,
Significance of plasmalemma aquaporins for water-transport in Arabidopsis thaliana.
1998,
Pubmed
,
Xenbase
Kase,
K-252 compounds, novel and potent inhibitors of protein kinase C and cyclic nucleotide-dependent protein kinases.
1987,
Pubmed
Kirch,
Expression of water channel proteins in Mesembryanthemum crystallinum.
2000,
Pubmed
Lian,
The role of aquaporin RWC3 in drought avoidance in rice.
2004,
Pubmed
,
Xenbase
Maggio,
Effects of Mercuric Chloride on the Hydraulic Conductivity of Tomato Root Systems (Evidence for a Channel-Mediated Water Pathway).
1995,
Pubmed
Martin,
Causes and consequences of high osmotic potentials in epiphytic higher plants.
2004,
Pubmed
Maurel,
Phosphorylation regulates the water channel activity of the seed-specific aquaporin alpha-TIP.
1995,
Pubmed
,
Xenbase
Nagata,
Gamma-radiation induces leaf trichome formation in Arabidopsis.
1999,
Pubmed
Nyman,
Active Uptake of Amino Acids by Leaves of an Epiphytic Vascular Plant, Tillandsia paucifolia (Bromeliaceae).
1987,
Pubmed
Ohshima,
Low aquaporin content and low osmotic water permeability of the plasma and vacuolar membranes of a CAM plant Graptopetalum paraguayense: comparison with radish.
2001,
Pubmed
Preston,
Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein.
1992,
Pubmed
,
Xenbase
Pérez-Estrada,
Variation in leaf trichomes of Wigandia urens: environmental factors and physiological consequences.
2000,
Pubmed
Shinohara,
How have the alpine dwarf plants in Yakushima been miniaturized? A comparative study of two alpine dwarf species in Yakushima, Blechnum niponicum (Blechnaceae) and Lysimachia japonica (Primulaceae).
2006,
Pubmed
Siefritz,
PIP1 plasma membrane aquaporins in tobacco: from cellular effects to function in plants.
2002,
Pubmed
,
Xenbase
Tischler,
Mimicry and inhibition of nerve growth factor effects: interactions of staurosporine, forskolin, and K252a in PC12 cells and normal rat chromaffin cells in vitro.
1990,
Pubmed
Tyerman,
Plant aquaporins: multifunctional water and solute channels with expanding roles.
2002,
Pubmed
Wagner,
New approaches for studying and exploiting an old protuberance, the plant trichome.
2004,
Pubmed
Yamada,
A family of transcripts encoding water channel proteins: tissue-specific expression in the common ice plant.
1995,
Pubmed
,
Xenbase
Yamaura,
Localization of the biosynthesis and accumulation of monoterpenoids in glandular trichomes of thyme.
1992,
Pubmed
Zhang,
Inhibition of water channels by HgCl2 in intact wheat root cells.
1999,
Pubmed
Zotz,
The physiological ecology of vascular epiphytes: current knowledge, open questions.
2001,
Pubmed
del Martínez-Ballesta,
Plant aquaporins: new perspectives on water and nutrient uptake in saline environment.
2006,
Pubmed