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XB-ART-35822
Methods 2007 Mar 01;413:291-5. doi: 10.1016/j.ymeth.2006.08.015.
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FRET-based methods to study ATP-dependent changes in chromatin structure.

Yang JG , Narlikar GJ .


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DNA packaging into chromatin imposes several levels of regulation on the central nuclear processes of DNA replication, recombination, repair and transcription. ATP-dependent chromatin-remodeling enzymes play a critical role in this regulation by altering the accessibility of nucleosomal DNA. Remodeling can result in large-scale changes in chromatin, such as the formation of heterochromatin, or smaller changes in exposure or occlusion of specific DNA regions. To understand the mechanisms of chromatin remodeling, we report a FRET-based method to follow remodeling of a single histone octamer on DNA. This technique provides a non-perturbing, solution-based approach to quantitatively track the movement of DNA with respect to the octamer in real-time. The method can easily be altered to examine other conformational changes within the nucleosome, and is applicable to study the enzymatic activity of several classes of chromatin-remodeling complexes.

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References [+] :
Aalfs, Functional differences between the human ATP-dependent nucleosome remodeling proteins BRG1 and SNF2H. 2001, Pubmed