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We deleted most of the pyruvate decarboxylase structural gene PDC1 from the genome of Saccharomyces cerevisiae. Surprisingly, mutants carrying this deletion allele showed a completely different phenotype than previously described point mutations. They were able to ferment glucose and their specific pyruvate decarboxylase activity was only reduced to 45% of the wild type level. Northern blot analysis revealed that a sequence in the yeast genome homologous to PDC1 and formerly designated as a possible pseudogene is expressed and may code for a different but closely related pyruvate decarboxylase. The products of the two PDC genes seem to form hybrid oligomers, however both homooligomers have enzyme activity. Thus, the product of the PDC1 gene is not absolutely necessary for glucose fermentation in yeast.
Barnett,
The utilization of sugars by yeasts.
1976, Pubmed
Barnett,
The utilization of sugars by yeasts.
1976,
Pubmed
Broach,
Construction of high copy yeast vectors using 2-microns circle sequences.
1983,
Pubmed
Butler,
Identification of an upstream activation site in the pyruvate decarboxylase structural gene (PDC1) of Saccharomyces cerevisiae.
1988,
Pubmed
Gounaris,
Pyruvate decarboxylase III. Specificity restrictions for thiamine pyrophosphate in the protein association step, sub-unit structure.
1975,
Pubmed
Grunstein,
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
1975,
Pubmed
Hohmann,
A region in the yeast genome which favours multiple integration of DNA via homologous recombination.
1987,
Pubmed
Hopmann,
Hydroxyl-ion-induced subunit dissociation of east cytoplasmic pyruvate decarboxylase. A circular dichroism study.
1980,
Pubmed
Kellermann,
The glucose-and ethanol-dependent regulation of PDC1 from Saccharomyces cerevisiae are controlled by two distinct promoter regions.
1988,
Pubmed
Kellermann,
Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae.
1986,
Pubmed
Kuo,
Resolution of brewers' yeast pyruvate decarboxylase into two isozymes.
1986,
Pubmed
Maitra,
A kinetic study of glycolytic enzyme synthesis in yeast.
1971,
Pubmed
Neigeborn,
Null mutations in the SNF3 gene of Saccharomyces cerevisiae cause a different phenotype than do previously isolated missense mutations.
1986,
Pubmed
Rothstein,
One-step gene disruption in yeast.
1983,
Pubmed
Schmitt,
Genetic analysis of the pyruvate decarboxylase reaction in yeast glycolysis.
1982,
Pubmed
Schmitt,
The synthesis of yeast pyruvate decarboxylase is regulated by large variations in the messenger RNA level.
1983,
Pubmed
Tautz,
An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels.
1983,
Pubmed
Vieira,
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
1982,
Pubmed
Zehender,
Improved purification of pyruvate decarboxylase from wheat germ. Its partial characterisation and comparison with the yeast enzyme.
1987,
Pubmed
Zimmermann,
Dominance and recessiveness at the protein level in mutant x wildtype crosses in Sacchaomyces cerevisiae.
1969,
Pubmed
