Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
SLC26A7 can function as a chloride-loading mechanism in parietal cells.
Kosiek O
,
Busque SM
,
Föller M
,
Shcheynikov N
,
Kirchhoff P
,
Bleich M
,
Muallem S
,
Geibel JP
.
???displayArticle.abstract???
To date three potential candidates for parietal cell basolateral Cl(-) entry have been described: the highly 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS)-sensitive Cl(-)/HCO(3)(-) exchanger AE2, the HCO(3)(-) and lowly DIDS-sensitive SLC26A7 protein, and the Na(+)-2Cl(-)K(+) cotransporter (NKCC1). In this study we investigate the contribution of these pathways to secretagogue stimulated acid secretion. Individually hand-dissected rat gastric glands were microfluorimetrically monitored for Cl(-) influx and pH(i) changes. Transporter activity was determined by varying ion content and through the use of pharmacological inhibitors. Expression of SLC26A7 in rat parietal cells was shown by immunohistochemistry and Western blot. SLC26A7 was inhibited by 5-Nitro-2-(3-phenylpropyl-amino)benzoic acid (NPPB) (100 microM) in the Xenopus laevis oocyte expression system. Cl(-) influx in parietal cells was enhanced by histamine, depended partially on endogenous HCO(3)(-) synthesis and completely on extracellular Na(+). Removal and subsequent readdition of Cl(-) revealed a low and a high DIDS-sensitive HCO(3)(-) extrusion system contributing to Cl(-) uptake. At acidic pH(i), however, H(+) extrusion via the H(+),K(+)-ATPase depending on Cl(-) uptake was abolished only in the presence of 100 microM (NPPB) and at high (250 microM) DIDS concentration. There was no effect of the NKCC inhibitor bumetanide on stimulated H(+) extrusion. These results would be compatible with SLC26A7 as a Cl(-) uptake system under histamine stimulation.
Alper,
Molecular physiology of SLC4 anion exchangers.
2006, Pubmed,
Xenbase
Alper,
Molecular physiology of SLC4 anion exchangers.
2006,
Pubmed
,
Xenbase
Ayalon,
Suppression of gastric acid secretion by furosemide in isolated gastric mucosa of guinea pig.
1980,
Pubmed
Busque,
L-type amino acids stimulate gastric acid secretion by activation of the calcium-sensing receptor in parietal cells.
2005,
Pubmed
Cheng,
Expression of an extracellular calcium-sensing receptor in rat stomach.
1999,
Pubmed
Dudas,
Immunolocalization of anion transporter Slc26a7 in mouse kidney.
2006,
Pubmed
Flagella,
Mice lacking the basolateral Na-K-2Cl cotransporter have impaired epithelial chloride secretion and are profoundly deaf.
1999,
Pubmed
Gawenis,
Mice with a targeted disruption of the AE2 Cl-/HCO3- exchanger are achlorhydric.
2004,
Pubmed
Geibel,
The stomach divalent ion-sensing receptor scar is a modulator of gastric acid secretion.
2001,
Pubmed
Grahammer,
The cardiac K+ channel KCNQ1 is essential for gastric acid secretion.
2001,
Pubmed
Haas,
The Na-K-Cl cotransporter of secretory epithelia.
2000,
Pubmed
Hersey,
Gastric acid secretion.
1995,
Pubmed
Hirschowitz,
Pharmacological aspects of acid secretion.
1995,
Pubmed
Jöns,
Basolateral localization of anion exchanger 2 (AE2) and actin in acid-secreting (parietal) cells of the human stomach.
1994,
Pubmed
Kim,
SLC26A7 is a Cl- channel regulated by intracellular pH.
2005,
Pubmed
,
Xenbase
Kirchhoff,
Characteristics of the K+-competitive H+,K+-ATPase inhibitor AZD0865 in isolated rat gastric glands.
2006,
Pubmed
Lytle,
Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies.
1995,
Pubmed
,
Xenbase
McDaniel,
Parietal cells express high levels of Na-K-2Cl cotransporter on migrating into the gastric gland neck.
1999,
Pubmed
McDaniel,
Role of Na-K-2Cl cotransporter-1 in gastric secretion of nonacidic fluid and pepsinogen.
2005,
Pubmed
Muallem,
Electrolyte transport across the basolateral membrane of the parietal cells.
1985,
Pubmed
Orloff,
Intestinal acid inhibits gastric acid secretion by neural and hormonal mechanisms in rats.
1992,
Pubmed
Paradiso,
Regulation of intracellular pH in resting and in stimulated parietal cells.
1989,
Pubmed
Paradiso,
Na-H and Cl-HCO3 exchange in rabbit oxyntic cells using fluorescence microscopy.
1987,
Pubmed
Petrovic,
Identification of an apical Cl-/HCO-3 exchanger in rat kidney proximal tubule.
2003,
Pubmed
,
Xenbase
Petrovic,
Identification of a basolateral Cl-/HCO3- exchanger specific to gastric parietal cells.
2003,
Pubmed
Prinz,
Acid secretion and the H,K ATPase of stomach.
1992,
Pubmed
Radebold,
Gap junctional channels regulate acid secretion in the mammalian gastric gland.
2001,
Pubmed
Rossmann,
Differential expression and regulation of AE2 anion exchanger subtypes in rabbit parietal and mucous cells.
2001,
Pubmed
Samuelson,
Insights into the regulation of gastric acid secretion through analysis of genetically engineered mice.
2003,
Pubmed
Seidler,
pHi and HCO3- dependence of proton extrusion and Cl(-)-base exchange rates in isolated rabbit parietal cells.
1994,
Pubmed
Singh,
An apical permeability barrier to NH3/NH4+ in isolated, perfused colonic crypts.
1995,
Pubmed
Soybel,
Role of basolateral Na(+)-K(+)-Cl- cotransport in HCl secretion by amphibian gastric mucosa.
1995,
Pubmed
Stuart-Tilley,
Immunolocalization of anion exchanger AE2 and cation exchanger NHE-1 in distinct adjacent cells of gastric mucosa.
1994,
Pubmed
Thomas,
Regulation of Cl/HCO3 exchange in gastric parietal cells.
1991,
Pubmed
Waisbren,
Luminal perfusion of isolated gastric glands.
1994,
Pubmed
Yao,
Cell biology of acid secretion by the parietal cell.
2003,
Pubmed