Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
RNA
2004 Jun 01;106:942-53. doi: 10.1261/rna.5256704.
Show Gene links
Show Anatomy links
Xenopus U3 snoRNA docks on pre-rRNA through a novel base-pairing interaction.
Borovjagin AV
,
Gerbi SA
.
???displayArticle.abstract???
U3 small nucleolar RNA (snoRNA) is essential for rRNA processing to form 18S ribosomal RNA (rRNA). Previously, it has been shown that nucleolin is needed to load U3 snoRNA on pre-rRNA. However, as documented here, this is not sufficient. We present data that base-pairing between the U3 hinges and the external transcribed spacer (ETS) is critical for functional alignment of U3 on its pre-rRNA substrate. Additionally, the interaction between the U3 hinges and the ETS is proposed to serve as an anchor to hold U3 on the pre-rRNA substrate, while box A at the 5' end of U3 snoRNA swivels from ETS contacts to 18S rRNA contacts. Compensatory base changes revealed base-pairing between the 3' hinge of U3 snoRNA and region E1 of the ETS in Xenopus pre-rRNA; this novel interaction is required for 18S rRNA production. In contrast, base-pairing between the 5' hinge of U3 snoRNA and region E2 of the ETS is auxiliary, unlike the case in yeast where it is required. Thus, higher and lower eukaryotes use different interactions for functional association of U3 with pre-rRNA. The U3 hinge sequence varies between species, but covariation in the ETS retains complementarity. This species-specific U3-pre-rRNA interaction offers a potential target for a new class of antibiotics to prevent ribosome biogenesis in eukaryotic pathogens.
Andersen,
Directed proteomic analysis of the human nucleolus.
2002, Pubmed
Andersen,
Directed proteomic analysis of the human nucleolus.
2002,
Pubmed
Beltrame,
Mutational analysis of an essential binding site for the U3 snoRNA in the 5' external transcribed spacer of yeast pre-rRNA.
1994,
Pubmed
Beltrame,
Identification and functional analysis of two U3 binding sites on yeast pre-ribosomal RNA.
1992,
Pubmed
,
Xenbase
Beltrame,
Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis.
1995,
Pubmed
Borovjagin,
U3 small nucleolar RNA is essential for cleavage at sites 1, 2 and 3 in pre-rRNA and determines which rRNA processing pathway is taken in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Borovjagin,
The spacing between functional Cis-elements of U3 snoRNA is critical for rRNA processing.
2000,
Pubmed
,
Xenbase
Borovjagin,
Xenopus U3 snoRNA GAC-Box A' and Box A sequences play distinct functional roles in rRNA processing.
2001,
Pubmed
,
Xenbase
Bourbon,
Role of phosphorylation on the maturation pathways of a 100 kDa nucleolar protein.
1983,
Pubmed
Dragon,
A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis.
2002,
Pubmed
Enright,
5'ETS rRNA processing facilitated by four small RNAs: U14, E3, U17, and U3.
1996,
Pubmed
Epstein,
Multiple states of U3 RNA in Novikoff hepatoma nucleoli.
1984,
Pubmed
Fatica,
Making ribosomes.
2002,
Pubmed
Gerbi,
Ribosome biogenesis: role of small nucleolar RNA in maturation of eukaryotic rRNA.
2001,
Pubmed
Ginisty,
Nucleolin functions in the first step of ribosomal RNA processing.
1998,
Pubmed
,
Xenbase
Ginisty,
Structure and functions of nucleolin.
1999,
Pubmed
Ginisty,
Interaction of nucleolin with an evolutionarily conserved pre-ribosomal RNA sequence is required for the assembly of the primary processing complex.
2000,
Pubmed
,
Xenbase
Grainger,
Dictyostelium ribosomal RNA is processed during transcription.
1980,
Pubmed
Grandi,
90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors.
2002,
Pubmed
Hanakahi,
The C-terminal domain of nucleolin accelerates nucleic acid annealing.
2000,
Pubmed
Hartshorne,
Distinct regions of U3 snoRNA interact at two sites within the 5' external transcribed spacer of pre-rRNAs in Trypanosoma brucei cells.
1998,
Pubmed
Hartshorne,
Two 5'-ETS regions implicated in interactions with U3 snoRNA are required for small subunit rRNA maturation in Trypanosoma brucei.
1999,
Pubmed
Hartshorne,
Trypanosoma brucei 5'ETS A'-cleavage is directed by 3'-adjacent sequences, but not two U3 snoRNA-binding elements, which are all required for subsequent pre-small subunit rRNA processing events.
2001,
Pubmed
Herrera,
Association of protein C23 with rapidly labeled nucleolar RNA.
1986,
Pubmed
Hughes,
Functional base-pairing interaction between highly conserved elements of U3 small nucleolar RNA and the small ribosomal subunit RNA.
1996,
Pubmed
Hughes,
Depletion of U3 small nucleolar RNA inhibits cleavage in the 5' external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA.
1991,
Pubmed
Jeppesen,
Nucleotide sequence determination and secondary structure of Xenopus U3 snRNA.
1988,
Pubmed
,
Xenbase
Kass,
Primary processing of mammalian rRNA involves two adjacent cleavages and is not species specific.
1987,
Pubmed
Kass,
The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing.
1990,
Pubmed
Kass,
The first pre-rRNA-processing event occurs in a large complex: analysis by gel retardation, sedimentation, and UV cross-linking.
1990,
Pubmed
Lange,
Nucleolar localization elements of Xenopus laevis U3 small nucleolar RNA.
1998,
Pubmed
,
Xenbase
Léger-Silvestre,
Ultrastructural changes in the Schizosaccharomyces pombe nucleolus following the disruption of the gar2+ gene, which encodes a nucleolar protein structurally related to nucleolin.
1997,
Pubmed
Maser,
U3 small nuclear RNA can be psoralen-cross-linked in vivo to the 5' external transcribed spacer of pre-ribosomal-RNA.
1989,
Pubmed
Michot,
Secondary structure of the 5' external transcribed spacer of vertebrate pre-rRNA. Presence of phylogenetically conserved features.
1991,
Pubmed
,
Xenbase
Mougey,
A U3 small nuclear ribonucleoprotein-requiring processing event in the 5' external transcribed spacer of Xenopus precursor rRNA.
1993,
Pubmed
,
Xenbase
Mougey,
The terminal balls characteristic of eukaryotic rRNA transcription units in chromatin spreads are rRNA processing complexes.
1993,
Pubmed
,
Xenbase
Méreau,
An in vivo and in vitro structure-function analysis of the Saccharomyces cerevisiae U3A snoRNP: protein-RNA contacts and base-pair interaction with the pre-ribosomal RNA.
1997,
Pubmed
Peculis,
snoRNA nuclear import and potential for cotranscriptional function in pre-rRNA processing.
2001,
Pubmed
,
Xenbase
Savino,
In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.
1990,
Pubmed
,
Xenbase
Savino,
Preribosomal RNA processing in Xenopus oocytes does not include cleavage within the external transcribed spacer as an early step.
1991,
Pubmed
,
Xenbase
Schnare,
The 28S-18S rDNA intergenic spacer from Crithidia fasciculata: repeated sequences, length heterogeneity, putative processing sites and potential interactions between U3 small nucleolar RNA and the ribosomal RNA precursor.
2000,
Pubmed
Sharma,
Base pairing between U3 small nucleolar RNA and the 5' end of 18S rRNA is required for pre-rRNA processing.
1999,
Pubmed
Sipos,
Nucleolin promotes secondary structure in ribosomal RNA.
1991,
Pubmed
Stroke,
The 5' end of U3 snRNA can be crosslinked in vivo to the external transcribed spacer of rat ribosomal RNA precursors.
1989,
Pubmed
Tyc,
A new interaction between the mouse 5' external transcribed spacer of pre-rRNA and U3 snRNA detected by psoralen crosslinking.
1992,
Pubmed
Venema,
Ribosome synthesis in Saccharomyces cerevisiae.
1999,
Pubmed
Warner,
The economics of ribosome biosynthesis in yeast.
1999,
Pubmed