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Methods
2006 Dec 01;404:331-8. doi: 10.1016/j.ymeth.2006.07.031.
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Methods to identify and functionally analyze factors that specifically recognize histone lysine methylation.
Sims RJ
,
Trojer P
,
Li G
,
Reinberg D
.
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Histone lysine methylation plays an important role in the regulation of gene expression and impacts many fundamental biological processes, such as cellular identity. Despite great efforts, the mechanisms behind the downstream consequences of histone methyl-recognition remain poorly understood. Here, we describe various methods to investigate specific histone lysine-methyl recognition, including the use of short peptides, histone octamers, and the more physiological nucleosomal arrays. We also discuss techniques that are well suited to assess functional aspects of binding as it relates to transcriptional regulation.
Bannister,
Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain.
2001, Pubmed
Bannister,
Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain.
2001,
Pubmed
Bednar,
Nucleosomes, linker DNA, and linker histone form a unique structural motif that directs the higher-order folding and compaction of chromatin.
1998,
Pubmed
Bernstein,
Genomic maps and comparative analysis of histone modifications in human and mouse.
2005,
Pubmed
Boccuni,
The human L(3)MBT polycomb group protein is a transcriptional repressor and interacts physically and functionally with TEL (ETV6).
2003,
Pubmed
Cao,
Role of histone H3 lysine 27 methylation in Polycomb-group silencing.
2002,
Pubmed
Czermin,
Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites.
2002,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Francis,
Chromatin compaction by a polycomb group protein complex.
2004,
Pubmed
Jacobs,
Assays for the determination of structure and dynamics of the interaction of the chromodomain with histone peptides.
2004,
Pubmed
Kim,
NAD+-dependent modulation of chromatin structure and transcription by nucleosome binding properties of PARP-1.
2004,
Pubmed
Kim,
Tudor, MBT and chromo domains gauge the degree of lysine methylation.
2006,
Pubmed
Klymenko,
A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities.
2006,
Pubmed
Kuzmichev,
Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein.
2002,
Pubmed
Lachner,
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.
2001,
Pubmed
Loyola,
Histone deposition and chromatin assembly by RSF.
2003,
Pubmed
Margueron,
The key to development: interpreting the histone code?
2005,
Pubmed
Nishioka,
Methods and tips for the purification of human histone methyltransferases.
2003,
Pubmed
,
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Pray-Grant,
Chd1 chromodomain links histone H3 methylation with SAGA- and SLIK-dependent acetylation.
2005,
Pubmed
Reinberg,
de FACTo nucleosome dynamics.
2006,
Pubmed
Schneider,
Histone H3 lysine 4 methylation patterns in higher eukaryotic genes.
2004,
Pubmed
Shi,
ING2 PHD domain links histone H3 lysine 4 methylation to active gene repression.
2006,
Pubmed
Simpson,
Structural features of a phased nucleosome core particle.
1983,
Pubmed
Sims,
Histone lysine methylation: a signature for chromatin function.
2003,
Pubmed
Sims,
Human but not yeast CHD1 binds directly and selectively to histone H3 methylated at lysine 4 via its tandem chromodomains.
2005,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Turner,
Histone acetylation and an epigenetic code.
2000,
Pubmed
Wysocka,
A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling.
2006,
Pubmed
,
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