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Mol Cell Biol
2006 Dec 01;2623:9045-59. doi: 10.1128/MCB.00248-06.
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The LRS and SIN domains: two structurally equivalent but functionally distinct nucleosomal surfaces required for transcriptional silencing.
Fry CJ
,
Norris A
,
Cosgrove M
,
Boeke JD
,
Peterson CL
.
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Genetic experiments have identified two structurally similar nucleosomal domains, SIN and LRS, required for transcriptional repression at genes regulated by the SWI/SNF chromatin remodeling complex or for heterochromatic gene silencing, respectively. Each of these domains consists of histone H3 and H4 L1 and L2 loops that form a DNA-binding surface at either superhelical location (SHL) +/-2.5 (LRS) or SHL +/-0.5 (SIN). Here we show that alterations in the LRS domain do not result in Sin(-) phenotypes, nor does disruption of the SIN domain lead to loss of ribosomal DNA heterochromatic gene silencing (Lrs(-) phenotype). Furthermore, whereas disruption of the SIN domain eliminates intramolecular folding of nucleosomal arrays in vitro, alterations in the LRS domain have no effect on chromatin folding in vitro. In contrast to these dissimilarities, we find that the SIN and LRS domains are both required for recruitment of Sir2p and Sir4p to telomeric and silent mating type loci, suggesting that both surfaces can contribute to heterochromatin formation. Our study shows that structurally similar nucleosomal surfaces provide distinct functionalities in vivo and in vitro.
Buck,
RNA polymerase I propagates unidirectional spreading of rDNA silent chromatin.
2002, Pubmed
Buck,
RNA polymerase I propagates unidirectional spreading of rDNA silent chromatin.
2002,
Pubmed
Carruthers,
Assembly of defined nucleosomal and chromatin arrays from pure components.
1999,
Pubmed
Cioci,
Silencing in yeast rDNA chromatin: reciprocal relationship in gene expression between RNA polymerase I and II.
2003,
Pubmed
Dong,
DNA and protein determinants of nucleosome positioning on sea urchin 5S rRNA gene sequences in vitro.
1990,
Pubmed
Duina,
Analysis of a mutant histone H3 that perturbs the association of Swi/Snf with chromatin.
2004,
Pubmed
Flaus,
Sin mutations alter inherent nucleosome mobility.
2004,
Pubmed
Fletcher,
Core histone tail domains mediate oligonucleosome folding and nucleosomal DNA organization through distinct molecular mechanisms.
1995,
Pubmed
Goldstein,
Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.
1999,
Pubmed
Gottschling,
Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription.
1990,
Pubmed
Hansen,
Homogeneous reconstituted oligonucleosomes, evidence for salt-dependent folding in the absence of histone H1.
1989,
Pubmed
Horn,
The SIN domain of the histone octamer is essential for intramolecular folding of nucleosomal arrays.
2002,
Pubmed
,
Xenbase
Kruger,
Amino acid substitutions in the structured domains of histones H3 and H4 partially relieve the requirement of the yeast SWI/SNF complex for transcription.
1995,
Pubmed
Kuo,
In vivo cross-linking and immunoprecipitation for studying dynamic Protein:DNA associations in a chromatin environment.
1999,
Pubmed
Kurumizaka,
Sin mutations of histone H3: influence on nucleosome core structure and function.
1997,
Pubmed
,
Xenbase
Lenfant,
All four core histone N-termini contain sequences required for the repression of basal transcription in yeast.
1996,
Pubmed
Logie,
Catalytic activity of the yeast SWI/SNF complex on reconstituted nucleosome arrays.
1997,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Luger,
DNA binding within the nucleosome core.
1998,
Pubmed
Luger,
Expression and purification of recombinant histones and nucleosome reconstitution.
1999,
Pubmed
Luo,
Rap1-Sir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeast.
2002,
Pubmed
Muthurajan,
Crystal structures of histone Sin mutant nucleosomes reveal altered protein-DNA interactions.
2004,
Pubmed
Ng,
Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association.
2002,
Pubmed
Park,
A core nucleosome surface crucial for transcriptional silencing.
2002,
Pubmed
Rusche,
The establishment, inheritance, and function of silenced chromatin in Saccharomyces cerevisiae.
2003,
Pubmed
Rusché,
Ordered nucleation and spreading of silenced chromatin in Saccharomyces cerevisiae.
2002,
Pubmed
Schnell,
A position effect on the expression of a tRNA gene mediated by the SIR genes in Saccharomyces cerevisiae.
1986,
Pubmed
Schwarz,
Formation and stability of higher order chromatin structures. Contributions of the histone octamer.
1994,
Pubmed
Schwarz,
Reversible oligonucleosome self-association: dependence on divalent cations and core histone tail domains.
1996,
Pubmed
Singer,
Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae.
1998,
Pubmed
Smith,
An unusual form of transcriptional silencing in yeast ribosomal DNA.
1997,
Pubmed
Smith,
Distribution of a limited Sir2 protein pool regulates the strength of yeast rDNA silencing and is modulated by Sir4p.
1998,
Pubmed
Sternberg,
Activation of the yeast HO gene by release from multiple negative controls.
1987,
Pubmed
Straight,
Net1, a Sir2-associated nucleolar protein required for rDNA silencing and nucleolar integrity.
1999,
Pubmed
Thompson,
Identification of a functional domain within the essential core of histone H3 that is required for telomeric and HM silencing in Saccharomyces cerevisiae.
2003,
Pubmed
Wechser,
Effects of Sin- versions of histone H4 on yeast chromatin structure and function.
1997,
Pubmed
van Leeuwen,
Dot1p modulates silencing in yeast by methylation of the nucleosome core.
2002,
Pubmed