Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Virol
2004 Jun 01;7812:6459-68. doi: 10.1128/JVI.78.12.6459-6468.2004.
Show Gene links
Show Anatomy links
Adenovirus protein VII condenses DNA, represses transcription, and associates with transcriptional activator E1A.
Johnson JS
,
Osheim YN
,
Xue Y
,
Emanuel MR
,
Lewis PW
,
Bankovich A
,
Beyer AL
,
Engel DA
.
???displayArticle.abstract???
Adenovirus protein VII is the major protein component of the viral nucleoprotein core. It is highly basic, and an estimated 1070 copies associate with each viral genome, forming a tightly condensed DNA-protein complex. We have investigated DNA condensation, transcriptional repression, and specific protein binding by protein VII. Xenopus oocytes were microinjected with mRNA encoding HA-tagged protein VII and prepared for visualization of lampbrush chromosomes. Immunostaining revealed that protein VII associated in a uniform manner across entire chromosomes. Furthermore, the chromosomes were significantly condensed and transcriptionally silenced, as judged by the dramatic disappearance of transcription loops characteristic of lampbrush chromosomes. During infection, the protein VII-DNA complex may be the initial substrate for transcriptional activation by cellular factors and the viral E1A protein. To investigate this possibility, mRNAs encoding E1A and protein VII were comicroinjected into Xenopus oocytes. Interestingly, whereas E1A did not associate with chromosomes in the absence of protein VII, expression of both proteins together resulted in significant association of E1A with lampbrush chromosomes. Binding studies with proteins produced in bacteria or human cells or by in vitro translation showed that E1A and protein VII can interact in vitro. Structure-function analysis revealed that an N-terminal region of E1A is responsible for binding to protein VII. These studies define the in vivo functions of protein VII in DNA binding, condensation, and transcriptional repression and indicate a role in E1A-mediated transcriptional activation of viral genes.
Amin,
Genetic analysis of adenovirus type 2. VII. Cleavage-modified affinity for DNA of internal virion proteins.
1977, Pubmed
Amin,
Genetic analysis of adenovirus type 2. VII. Cleavage-modified affinity for DNA of internal virion proteins.
1977,
Pubmed
Anderson,
Characterization of the adenovirus 2 virion protein, mu.
1989,
Pubmed
Arany,
A family of transcriptional adaptor proteins targeted by the E1A oncoprotein.
1995,
Pubmed
Berk,
Mechanisms of viral activators.
1998,
Pubmed
Black,
DNA-binding properties of the major core protein of adenovirus 2.
1979,
Pubmed
Boyer,
Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein.
1999,
Pubmed
Brown,
Structure and composition of the adenovirus type 2 core.
1975,
Pubmed
Burnham,
The identification of p130cas-binding proteins and their role in cellular transformation.
1996,
Pubmed
Cadieux,
Identification of the periplasmic cobalamin-binding protein BtuF of Escherichia coli.
2002,
Pubmed
Chakravarti,
A viral mechanism for inhibition of p300 and PCAF acetyltransferase activity.
1999,
Pubmed
Chatterjee,
Adenoviral protein VII packages intracellular viral DNA throughout the early phase of infection.
1986,
Pubmed
Chatterjee,
DNA-binding properties of an adenovirus 289R E1A protein.
1988,
Pubmed
Corden,
Chromatin-like organization of the adenovirus chromosome.
1976,
Pubmed
Daniell,
Adenovirus chromatin structure at different stages of infection.
1981,
Pubmed
Déry,
The structure of adenovirus chromatin in infected cells.
1985,
Pubmed
Ennever,
Ionic effects on the structure of nucleoprotein cores from adenovirus.
1985,
Pubmed
Everitt,
Structural proteins of adenoviruses. X. Isolation and topography of low molecular weight antigens from the virion of adenovirus type 2.
1973,
Pubmed
Everitt,
Structural proteins of adenoviruses. XII. Location and neighbor relationship among proteins of adenovirion type 2 as revealed by enzymatic iodination, immunoprecipitation and chemical cross-linking.
1975,
Pubmed
Fax,
cAMP-independent activation of the adenovirus type 12 E2 promoter correlates with the recruitment of CREB-1/ATF-1, E1A(12S), and CBP to the E2-CRE.
2000,
Pubmed
Flint,
Viral transactivating proteins.
1997,
Pubmed
Frisch,
Adenovirus-5 E1A: paradox and paradigm.
2002,
Pubmed
Fuchs,
The p400 complex is an essential E1A transformation target.
2001,
Pubmed
Gall,
Assembly of the nuclear transcription and processing machinery: Cajal bodies (coiled bodies) and transcriptosomes.
1999,
Pubmed
,
Xenbase
Gall,
Lampbrush chromosomes.
1991,
Pubmed
,
Xenbase
Greber,
The role of the nuclear pore complex in adenovirus DNA entry.
1997,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Haley,
Transformation properties of type 5 adenovirus mutants that differentially express the E1A gene products.
1984,
Pubmed
Harlow,
Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products.
1985,
Pubmed
Hosokawa,
Isolation and characterization of an extremely basic protein from adenovirus type 5.
1976,
Pubmed
Jones,
Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells.
1979,
Pubmed
Jones,
An adenovirus type 5 early gene function regulates expression of other early viral genes.
1979,
Pubmed
Keller,
Biophysical characterization of the DNA binding and condensing properties of adenoviral core peptide mu.
2002,
Pubmed
Korn,
Adenovirus DNA synthesis in vitro is inhibited by the virus-coded major core protein.
1986,
Pubmed
Kraus,
Interaction of the Dr1 inhibitory factor with the TATA binding protein is disrupted by adenovirus E1A.
1994,
Pubmed
Lai,
Ethidium bromide provides a simple tool for identifying genuine DNA-independent protein associations.
1992,
Pubmed
Lang,
The adenovirus E1A oncoprotein recruits the cellular TRRAP/GCN5 histone acetyltransferase complex.
2003,
Pubmed
Leff,
Individual products of the adenovirus 12S and 13S EIa mRNAs stimulate viral EIIa and EIII expression at the transcriptional level.
1984,
Pubmed
Maizel,
The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion.
1968,
Pubmed
Matthews,
Adenovirus core protein V is delivered by the invading virus to the nucleus of the infected cell and later in infection is associated with nucleoli.
1998,
Pubmed
Miller,
Adenovirus E1A specifically blocks SWI/SNF-dependent transcriptional activation.
1996,
Pubmed
Mirza,
Structure of adenovirus chromatin.
1982,
Pubmed
Murphy,
Human SWI-SNF component BRG1 represses transcription of the c-fos gene.
1999,
Pubmed
Mymryk,
Multiple pathways for gene activation in rodent cells by the smaller adenovirus 5 E1A protein and their relevance to growth and transformation.
1993,
Pubmed
Nakanishi,
In vitro transcription of a chromatin-like complex of major core protein VII and DNA of adenovirus serotype 2.
1986,
Pubmed
Nermut,
Electron microscopy of adenovirus cores.
1975,
Pubmed
Newcomb,
Ion etching of human adenovirus 2: structure of the core.
1984,
Pubmed
Osheim,
EM visualization of transcriptionally active genes after injection into Xenopus oocyte nuclei.
1998,
Pubmed
,
Xenbase
Prage,
Structural proteins of adenoviruses. VII. Purification and properties of an arginine-rich core protein from adenovirus type 2 and type 3.
1971,
Pubmed
Rekosh,
Identification of a protein linked to the ends of adenovirus DNA.
1977,
Pubmed
Russell,
Internal components of adenovirus.
1968,
Pubmed
Russell,
Nucleic acid-binding properties of adenovirus structural polypeptides.
1982,
Pubmed
Sergeant,
Nucleosome-like structural subunits of intranuclear parental adenovirus type 2 DNA.
1979,
Pubmed
Tate,
Parental adenovirus DNA accumulates in nucleosome-like structures in infected cells.
1979,
Pubmed
Vayda,
The structure of nucleoprotein cores released from adenovirions.
1983,
Pubmed
Vayda,
Transcription of adenovirus cores in vitro.
1984,
Pubmed
Vayda,
Isolation and characterization of adenovirus core nucleoprotein subunits.
1987,
Pubmed
Weber,
Adenovirus core proteins.
1983,
Pubmed
Wong,
Linear adenovirus DNA is organized into supercoiled domains in virus particles.
1989,
Pubmed
Wong,
Complementary functions of E1a conserved region 1 cooperate with conserved region 3 to activate adenovirus serotype 5 early promoters.
1994,
Pubmed
Zhang,
Role for the adenovirus IVa2 protein in packaging of viral DNA.
2001,
Pubmed
Zu,
Separable roles in vivo for the two RNA binding domains of Drosophila A1-hnRNP homolog.
1998,
Pubmed