Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nucleic Acids Res
2002 Aug 01;3015:3490-6. doi: 10.1093/nar/gkf461.
Show Gene links
Show Anatomy links
Ribozyme suppression of endogenous thyroid hormone receptor activity in Xenopus laevis cells.
Lim W
,
Furlow JD
.
???displayArticle.abstract???
Xenopus laevis is an excellent model for thyroid hormone (T3)-regulated gene expression. T3 initiates two drastically different pathways during metamorphosis: death of larval tissues and growth of adult tissues. The role that each T3 receptor (TR) isotype, alpha and beta, plays in metamorphosis is uncertain. The X.laevis tetraploid genome limits experiments to overexpression, misexpression and dominant negative studies. Ribozymes offer an alternative by suppressing gene activity through specific mRNA reduction. It has been suggested that ribozymes will not work in X.laevis because of the organism's intracellular environment and body temperature. In this study, we show that hammerhead ribozymes are active in vitro against transcribed TRbeta message and in vivo against a TRbeta-luciferase fusion protein. We next show that TRbeta-targeted ribozymes can inhibit T3-induced transcription of a reporter gene in cultured X.laevis cells, using T3 response elements from two T3-responsive transcription factor genes. One has early expression kinetics in response to T3 and is proposed to be TRalpha regulated whereas the other has intermediate induction kinetics and thus may be partially TRbeta regulated. Therefore, ribozymes are a potentially valuable tool for overcoming the limitations in this system for examining gene function in X.laevis.
Banker,
The thyroid hormone receptor gene (c-erbA alpha) is expressed in advance of thyroid gland maturation during the early embryonic development of Xenopus laevis.
1991, Pubmed,
Xenbase
Banker,
The thyroid hormone receptor gene (c-erbA alpha) is expressed in advance of thyroid gland maturation during the early embryonic development of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Berry,
The expression pattern of thyroid hormone response genes in remodeling tadpole tissues defines distinct growth and resorption gene expression programs.
1998,
Pubmed
,
Xenbase
Brown,
The thyroid hormone-induced tail resorption program during Xenopus laevis metamorphosis.
1996,
Pubmed
,
Xenbase
Chiellini,
A high-affinity subtype-selective agonist ligand for the thyroid hormone receptor.
1998,
Pubmed
Conaty,
Selected classes of minimised hammerhead ribozyme have very high cleavage rates at low Mg2+ concentration.
1999,
Pubmed
Eliceiri,
Quantitation of endogenous thyroid hormone receptors alpha and beta during embryogenesis and metamorphosis in Xenopus laevis.
1994,
Pubmed
,
Xenbase
Ermakova,
The homeobox gene, Xanf-1, can control both neural differentiation and patterning in the presumptive anterior neurectoderm of the Xenopus laevis embryo.
1999,
Pubmed
,
Xenbase
Flatman,
Mechanisms of magnesium transport.
1991,
Pubmed
Furlow,
In vitro and in vivo analysis of the regulation of a transcription factor gene by thyroid hormone during Xenopus laevis metamorphosis.
1999,
Pubmed
,
Xenbase
Haseloff,
Simple RNA enzymes with new and highly specific endoribonuclease activities.
1988,
Pubmed
Kanamori,
The regulation of thyroid hormone receptor beta genes by thyroid hormone in Xenopus laevis.
1992,
Pubmed
,
Xenbase
Kanamori,
Cultured cells as a model for amphibian metamorphosis.
1993,
Pubmed
,
Xenbase
Kinugawa,
Regulation of thyroid hormone receptor isoforms in physiological and pathological cardiac hypertrophy.
2001,
Pubmed
Saxena,
Ribozymes correctly cleave a model substrate and endogenous RNA in vivo.
1990,
Pubmed
,
Xenbase
Schmidt,
RNA double cleavage by a hairpin-derived twin ribozyme.
2000,
Pubmed
Schreiber,
Diverse developmental programs of Xenopus laevis metamorphosis are inhibited by a dominant negative thyroid hormone receptor.
2001,
Pubmed
,
Xenbase
Szyrach,
Cleavage of AML1/MTG8 by asymmetric hammerhead ribozymes.
2001,
Pubmed
,
Xenbase
Trost,
The thyroid hormone receptor-beta-selective agonist GC-1 differentially affects plasma lipids and cardiac activity.
2000,
Pubmed
Ulisse,
Dominant-negative mutant thyroid hormone receptors prevent transcription from Xenopus thyroid hormone receptor beta gene promoter in response to thyroid hormone in Xenopus tadpoles in vivo.
1996,
Pubmed
,
Xenbase
Vize,
Assays for gene function in developing Xenopus embryos.
1991,
Pubmed
,
Xenbase
Wang,
Thyroid hormone-induced gene expression program for amphibian tail resorption.
1993,
Pubmed
,
Xenbase
Wong,
A role for nucleosome assembly in both silencing and activation of the Xenopus TR beta A gene by the thyroid hormone receptor.
1995,
Pubmed
,
Xenbase
Yaoita,
Xenopus laevis alpha and beta thyroid hormone receptors.
1990,
Pubmed
,
Xenbase
Yaoita,
A correlation of thyroid hormone receptor gene expression with amphibian metamorphosis.
1990,
Pubmed
,
Xenbase
Zhang,
The mechanism of action of thyroid hormones.
2000,
Pubmed
