Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Membr Biol
2004 Nov 01;2021:39-49. doi: 10.1007/s00232-004-0718-3.
Show Gene links
Show Anatomy links
Lanthanum effect on the dynamics of tight junction opening and closing.
Lacaz-Vieira F
,
Marques MM
.
???displayArticle.abstract???
We present a comparative study in frog urinary bladders (FUB) and A6 cell monolayers (A6CM) on the effect of La3+ on tight junction (TJ) dynamics. These tissues react similarly to changes of basolateral Ca2+ (Ca(2+)bl), while responding differently to the action of La3+(bl). In FUB, La(3+)bl shows a Ca(2+)-antagonistic effect that promotes TJ opening in the presence of a normal Ca(2+)bl concentration. In A6CM, in contrast, La(3+)bl always shows a clear Ca(2+)-agonistic effect. The fact that a concentration of La(3+)bl one fifth of the normal Ca(2+)bl leads in FUB to TJ opening and in A6CM to a complete recovery of the TJ seal indicates a high affinity of La3+ for the Ca(2+)-binding sites in both tissues. In FUB, apical La3+ (La(3+)ap) exhibits, differently from its basolateral effect, an evident Ca(2+)-agonistic effect, suggesting a dual effect of La3+, depending on which side of the bladder La3+ is applied. In A6CM La(3+)ap has a Ca(2+)-agonistic effect similar to La(3+)bl. The effects of La(3+)bl in FUB and in A6CM are consistent, according to our previous publications, with La3+ acting antagonistically or agonistically, respectively, on the Ca2+ binding sites of zonula adhaerens. Despite the fact that the effect of La(3+)ap is clear in both tissues, its site of action is yet to be determined. Protonation of the Ca(2+)-binding sites causes a decrease of its agonistic effect on A6CM, consistent with a negatively charged binding site. In A6CM La3+ apparently replaces Ca2+, mimicking the effect of Ca2+ triggering the cascade of events leading to TJ closure. In FUB, La3+ interacts with the binding sites, dislodging Ca2+, with a high affinity, but this interaction is inadequate to initiate or sustain the process of junction closing. Possibly, the difference between the two preparations resides in subtle conformation differences of the outer segment of E-cadherin molecules.
Alvarado,
Chloride transport across isolated skin of Rana pipiens.
1975, Pubmed
Alvarado,
Chloride transport across isolated skin of Rana pipiens.
1975,
Pubmed
Anderson,
Tight junctions: closing in on the seal.
,
Pubmed
Armstrong,
Modification of sodium channel gating by lanthanum. Some effects that cannot be explained by surface charge theory.
1990,
Pubmed
Balda,
Assembly and sealing of tight junctions: possible participation of G-proteins, phospholipase C, protein kinase C and calmodulin.
1991,
Pubmed
Balda,
Structure, regulation, and pathophysiology of tight junctions in the gastrointestinal tract.
1992,
Pubmed
Balda,
Assembly of the tight junction: the role of diacylglycerol.
1993,
Pubmed
Boller,
Cell-adhesion molecule uvomorulin is localized in the intermediate junctions of adult intestinal epithelial cells.
1985,
Pubmed
Brown,
Calcium modulation of adherens and tight junction function: a potential mechanism for blood-brain barrier disruption after stroke.
2002,
Pubmed
Castro,
Deposition of BaSO4 in the tight junctions of amphibian epithelia causes their opening; apical Ca2+ reverses this effect.
1993,
Pubmed
Cereijido,
Structural and functional membrane polarity in cultured monolayers of MDCK cells.
1980,
Pubmed
Cereijido,
Occluding junctions in cultured epithelial monolayers.
1981,
Pubmed
Cereijido,
Role of tight junctions in establishing and maintaining cell polarity.
1998,
Pubmed
Clarke,
Adrenocorticotropic hormone stimulates a transient calcium uptake in rat lymphocytes.
1994,
Pubmed
Galli,
Extracellular calcium and the organization of tight junctions in pancreatic acinar cells.
1976,
Pubmed
García-Colunga,
Opposite effects of lanthanum on different types of nicotinic acetylcholine receptors.
1997,
Pubmed
,
Xenbase
Gleeson,
Sustaining olfaction at low salinities: evidence for a paracellular route of ion movement from the hemolymph to the sensillar lymph in the olfactory sensilla of the blue crab Callinectes sapidus.
2000,
Pubmed
Gonzalez-Mariscal,
Tight junction formation in cultured epithelial cells (MDCK).
1985,
Pubmed
González-Mariscal,
Tight junction proteins.
2003,
Pubmed
,
Xenbase
Gottardi,
E-cadherin suppresses cellular transformation by inhibiting beta-catenin signaling in an adhesion-independent manner.
2001,
Pubmed
Gumbiner,
The role of the cell adhesion molecule uvomorulin in the formation and maintenance of the epithelial junctional complex.
1988,
Pubmed
Hays,
The effect of calcium withdrawal on the structure and function of the toad bladder.
1965,
Pubmed
Hille,
Negative surface charge near sodium channels of nerve: divalent ions, monovalent ions, and pH.
1975,
Pubmed
Jan,
Mechanism of rise and decay of thapsigargin-evoked calcium signals in MDCK cells.
1999,
Pubmed
Kassab,
Modeling tight junction dynamics and oscillations.
2002,
Pubmed
Kemler,
Classical cadherins.
1992,
Pubmed
Koch,
Calcium binding and homoassociation of E-cadherin domains.
1997,
Pubmed
Lacaz-Vieira,
Calcium site specificity. Early Ca2+-related tight junction events.
1997,
Pubmed
Lacaz-Vieira,
Sodium flux in the apical membrane of the toad skin: aspects of its regulation and the importance of the ionic strength of the outer solution upon the reversibility of amiloride inhibition.
1986,
Pubmed
Lacaz-Vieira,
Small synthetic peptides homologous to segments of the first external loop of occludin impair tight junction resealing.
1999,
Pubmed
Lacaz-Vieira,
Protein kinase inhibitors and the dynamics of tight junction opening and closing in A6 cell monolayers.
2001,
Pubmed
,
Xenbase
Lacaz-Vieira,
Tight junction dynamics: oscillations and the role of protein kinase C.
2000,
Pubmed
Lacaz-Vieira,
Tight junction dynamics in the frog urinary bladder.
1996,
Pubmed
Lippoldt,
Structural alterations of tight junctions are associated with loss of polarity in stroke-prone spontaneously hypertensive rat blood-brain barrier endothelial cells.
2000,
Pubmed
Lora,
Hepatocyte tight-junctional permeability is increased in rat experimental colitis.
1997,
Pubmed
Machen,
Permeable junctional complexes. The movement of lanthanum across rabbit gallbladder and intestine.
1972,
Pubmed
Martinez-Palomo,
Experimental modulation of occluding junctions in a cultured transporting epithelium.
1980,
Pubmed
Mazzon,
Effect of stress on the paracellular barrier in the rat ileum.
2002,
Pubmed
Meldolesi,
Ca++-dependent disassembly and reassembly of occluding junctions in guinea pig pancreatic acinar cells. Effect of drugs.
1978,
Pubmed
Nagar,
Structural basis of calcium-induced E-cadherin rigidification and dimerization.
1996,
Pubmed
Nelson,
Effects of permeant ions and blockers on properties of single calcium channels from brains.
1987,
Pubmed
Palant,
Ca2+ regulation of tight-junction permeability and structure in Necturus gallbladder.
1983,
Pubmed
Pitelka,
Effects of extracellular calcium depletion on membrane topography and occluding junctions of mammary epithelial cells in culture.
1983,
Pubmed
Poncet,
A calcium-permeable channel in the apical membrane of primary cultures of the rabbit distal bright convoluted tubule.
1992,
Pubmed
Powis,
Lanthanum can be transported by the sodium-calcium exchange pathway and directly triggers catecholamine release from bovine chromaffin cells.
1994,
Pubmed
Ramsey,
Indomethacin blocks enhanced paracellular backflux in proximal tubules.
2002,
Pubmed
Ramsey,
Effect of volume expansion on the paracellular flux of lanthanum in the proximal tubule.
1998,
Pubmed
Ranscht,
Cadherins and catenins: interactions and functions in embryonic development.
1994,
Pubmed
Ringwald,
The structure of cell adhesion molecule uvomorulin. Insights into the molecular mechanism of Ca2+-dependent cell adhesion.
1987,
Pubmed
SEDAR,
EFFECTS OF CALCIUM DEPLETION ON THE JUNCTIONAL COMPLEX BETWEEN OXYNTIC CELLS OF GASTRIC GLANDS.
1964,
Pubmed
Schneeberger,
The tight junction: a multifunctional complex.
2004,
Pubmed
Shapiro,
Structural basis of cell-cell adhesion by cadherins.
1995,
Pubmed
Snyder,
Calcium(II) site specificity: effect of size and charge on metal ion binding to an EF-hand-like site.
1990,
Pubmed
Suzuki,
SS1 Helicobacter pylori disrupts the paracellular barrier of the gastric mucosa and leads to neutrophilic gastritis in mice.
2002,
Pubmed
Takata,
Ionic conductance changes in lobster axon membrane when lanthanum is substituted for calcium.
1966,
Pubmed
Takeichi,
Cadherins: a molecular family important in selective cell-cell adhesion.
1990,
Pubmed
Tomschy,
Homophilic adhesion of E-cadherin occurs by a co-operative two-step interaction of N-terminal domains.
1996,
Pubmed
Uehara,
Tight junction of sinus endothelial cells of the rat spleen.
1999,
Pubmed
Vogel,
Calcium and lanthanum effects at the nodal membrane.
1974,
Pubmed
,
Xenbase
Watkins,
Modulation of the gating of the transient outward potassium current of rat isolated cerebellar granule neurons by lanthanum.
1994,
Pubmed
Weiss,
Transient increase in lung epithelial tight junction permeability: an additional mechanism for enhancement of lung transgene expression by perfluorochemical liquids.
2003,
Pubmed
Yap,
Lateral clustering of the adhesive ectodomain: a fundamental determinant of cadherin function.
1997,
Pubmed
,
Xenbase
Zheng,
Expression, purification, and structural study of the EC4 domain of E-cadherin.
2004,
Pubmed