Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biochim Biophys Acta
2006 Jun 01;17586:781-8. doi: 10.1016/j.bbamem.2006.05.005.
Show Gene links
Show Anatomy links
Ala-504 is a determinant of substrate binding affinity in the mouse Na(+)/dicarboxylate cotransporter.
Oshiro N
,
Pajor AM
.
???displayArticle.abstract???
The Na(+)/dicarboxylate cotransporters from mouse (mNaDC1) and rabbit (rbNaDC1) differ in their ability to handle adipate, a six-carbon terminal dicarboxylic acid. The mNaDC1 and rbNaDC1 amino acid sequences are 75% identical. The rbNaDC1 does not transport adipate and only succinate produced inward currents under two-electrode voltage clamp. In contrast, oocytes expressing mNaDC1 had adipate-dependent inward currents that were about 60% of those induced by succinate. In order to identify domains involved in adipate transport, we examined the functional properties of a series of chimeric transporters made between mouse and rabbit NaDC1. We find that multiple transmembrane helices (TM), particularly TM 8, 9, and 10, are involved in adipate transport. In TM 10 there is only one amino acid difference between the two proteins, corresponding to Ala-504 in mouse and Ser-512 in rabbit NaDC1. The mNaDC1-A504S mutant had decreased adipate-dependent currents relative to succinate-dependent currents and an increase in the K(0.5) for both succinate and glutarate. We conclude that multiple amino acids from TM 8, 9 and 10 contribute to the transport of adipate in NaDC1. Furthermore, Ala-504 in TM 10 is an important determinant of K(0.5) for both adipate and succinate.
Bailey,
How to Make Drugs Orally Active: A Substrate Template for Peptide Transporter PepT1.
2000, Pubmed
Bailey,
How to Make Drugs Orally Active: A Substrate Template for Peptide Transporter PepT1.
2000,
Pubmed
Ballesteros,
Serine and threonine residues bend alpha-helices in the chi(1) = g(-) conformation.
2000,
Pubmed
Blostein,
Evidence that Ser775 in the alpha subunit of the Na,K-ATPase is a residue in the cation binding pocket.
1997,
Pubmed
Burckhardt,
Potential-dependent steady-state kinetics of a dicarboxylate transporter cloned from winter flounder kidney.
2000,
Pubmed
,
Xenbase
Burckhardt,
Substrate specificity of the human renal sodium dicarboxylate cotransporter, hNaDC-3, under voltage-clamp conditions.
2005,
Pubmed
,
Xenbase
Chen,
Characterization of a rat Na+-dicarboxylate cotransporter.
1998,
Pubmed
,
Xenbase
Chen,
Molecular and functional analysis of SDCT2, a novel rat sodium-dependent dicarboxylate transporter.
1999,
Pubmed
,
Xenbase
Cohen,
MAD phasing with krypton.
2001,
Pubmed
Dantzler,
Effect of alpha-KG in lumen on PAH transport by isolated perfused rabbit renal proximal tubules.
1996,
Pubmed
Fei,
Structural and functional characteristics of two sodium-coupled dicarboxylate transporters (ceNaDC1 and ceNaDC2) from Caenorhabditis elegans and their relevance to life span.
2003,
Pubmed
Geck,
Coupling in secondary transport. Effect of electrical potentials on the kinetics of ion linked co-transport.
1976,
Pubmed
Griffith,
Acidic residues involved in cation and substrate interactions in the Na+/dicarboxylate cotransporter, NaDC-1.
1999,
Pubmed
,
Xenbase
Kahn,
Determinants of substrate and cation affinities in the Na+/dicarboxylate cotransporter.
1999,
Pubmed
Li,
Serines 260 and 288 are involved in sulfate transport by hNaSi-1.
2003,
Pubmed
,
Xenbase
Mackenzie,
Mechanisms of the human intestinal H+-coupled oligopeptide transporter hPEPT1.
1996,
Pubmed
,
Xenbase
Mager,
Steady states, charge movements, and rates for a cloned GABA transporter expressed in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Merickel,
Identification of residues involved in substrate recognition by a vesicular monoamine transporter.
1995,
Pubmed
Mohana Rao,
A conformational preference parameter to predict helices in integral membrane proteins.
1986,
Pubmed
Nguyen,
Conformations, energies, and intramolecular hydrogen bonds in dicarboxylic acids: implications for the design of synthetic dicarboxylic acid receptors.
2005,
Pubmed
Oshiro,
Transmembrane helices 3 and 4 are involved in substrate recognition by the Na+/dicarboxylate cotransporter, NaDC1.
2006,
Pubmed
,
Xenbase
Oshiro,
Functional characterization of high-affinity Na(+)/dicarboxylate cotransporter found in Xenopus laevis kidney and heart.
2005,
Pubmed
,
Xenbase
Pajor,
Sodium and lithium interactions with the Na+/Dicarboxylate cotransporter.
1998,
Pubmed
,
Xenbase
Pajor,
Cloning and functional characterization of a high-affinity Na(+)/dicarboxylate cotransporter from mouse brain.
2001,
Pubmed
,
Xenbase
Pajor,
Molecular cloning, chromosomal organization, and functional characterization of a sodium-dicarboxylate cotransporter from mouse kidney.
2000,
Pubmed
,
Xenbase
Pajor,
Conformationally sensitive residues in transmembrane domain 9 of the Na+/dicarboxylate co-transporter.
2001,
Pubmed
,
Xenbase
Pajor,
Conformationally sensitive residues in extracellular loop 5 of the Na+/dicarboxylate co-transporter.
2005,
Pubmed
Pajor,
Characterization of the rabbit renal Na(+)-dicarboxylate cotransporter using antifusion protein antibodies.
1996,
Pubmed
,
Xenbase
Pajor,
Functional differences between rabbit and human Na(+)-dicarboxylate cotransporters, NaDC-1 and hNaDC-1.
1996,
Pubmed
,
Xenbase
Pajor,
Molecular properties of the SLC13 family of dicarboxylate and sulfate transporters.
2006,
Pubmed
Pak,
Etiology and treatment of urolithiasis.
1991,
Pubmed
Rogina,
Extended life-span conferred by cotransporter gene mutations in Drosophila.
2000,
Pubmed
Sur,
A single serine residue controls the cation dependence of substrate transport by the rat serotonin transporter.
1997,
Pubmed
Wang,
Structure, function, and genomic organization of human Na(+)-dependent high-affinity dicarboxylate transporter.
2000,
Pubmed
,
Xenbase
Yao,
Arginine-349 and aspartate-373 of the Na(+)/dicarboxylate cotransporter are conformationally sensitive residues.
2002,
Pubmed
Yao,
The transport properties of the human renal Na(+)- dicarboxylate cotransporter under voltage-clamp conditions.
2000,
Pubmed
,
Xenbase
Zhang,
Topology of the Na(+)/dicarboxylate cotransporter: the N-terminus and hydrophilic loop 4 are located intracellularly.
2001,
Pubmed
