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The centromere is the region within a chromosome that is required for proper segregation during mitosis and meiosis. Lesions in this sequence represent a unique type of damage, as loss of function could result in catastrophic loss of the genetic material of an entire chromosome. We have measured the induction by ultraviolet (UV) light of pyrimidine dimers in a 2550-bp restriction fragment that includes the centromere region of chromosome III in Saccharomyces cerevisiae. Yeast cells were exposed to ultraviolet light, cellular DNA was gently extracted, and subsequently treated with a UV-specific endonuclease to cleave all pyrimidine dimers. The sites of UV-specific nuclease scission within the centromere were determined by separating the DNA according to molecular weight, transferring the fragments to nitrocellulose, and hybridizing to a radiolabeled 624-bp fragment homologous to the centromere DNA from chromosome III. Several hotspots were identified in chromatin DNA from cells, as well as in irradiated deproteinized DNA. Double strand damage due to closely opposed pyrimidine dimers was also observed. At biological doses (35% survival) there are approximately 0.1 to 0.2 pyrimidine dimers per centromere. These dimers are efficiently repaired in the centromere and surrounding region.
Bloom,
Chromatin conformation of yeast centromeres.
1984, Pubmed,
Xenbase
Bloom,
Chromatin conformation of yeast centromeres.
1984,
Pubmed
,
Xenbase
Bloom,
Yeast centromere DNA is in a unique and highly ordered structure in chromosomes and small circular minichromosomes.
1982,
Pubmed
Bloom,
Structural analysis and sequence organization of yeast centromeres.
1983,
Pubmed
Bohr,
DNA repair in an active gene: removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall.
1985,
Pubmed
Carrier,
Endonuclease from Micrococcus luteus which has activity toward ultraviolet-irradiated deoxyribonucleic acid: purification and properties.
1970,
Pubmed
Clarke,
Isolation of a yeast centromere and construction of functional small circular chromosomes.
1980,
Pubmed
Clarke,
Genomic substitutions of centromeres in Saccharomyces cerevisiae.
,
Pubmed
Esposito,
Simultaneous detection of changes in chromosome number, gene conversion and intergenic recombination during mitosis of Saccharomyces cerevisiae: spontaneous and ultraviolet light induced events.
1982,
Pubmed
Evans,
Excision repair of pyrimidine dimers from simian virus 40 minichromosomes in vitro.
1984,
Pubmed
Fitzgerald-Hayes,
Nucleotide sequence comparisons and functional analysis of yeast centromere DNAs.
1982,
Pubmed
Forte,
Naturally occurring cross-links in yeast chromosomal DNA.
1976,
Pubmed
Gaudet,
Alterations in the adenine-plus-thymine-rich region of CEN3 affect centromere function in Saccharomyces cerevisiae.
1987,
Pubmed
Hieter,
Functional selection and analysis of yeast centromeric DNA.
1985,
Pubmed
Lam,
Bifilar enzyme-sensitive sites in ultraviolet-irradiated DNA are indicative of closely opposed cyclobutyl pyrimidine dimers.
1986,
Pubmed
Lam,
A sensitive, enzymatic assay for the detection of closely opposed cyclobutyl pyrimidine dimers induced in human diploid fibroblasts.
1986,
Pubmed
Leblon,
Most Uv-Induced Reciprocal Translocations in SORDARIA MACROSPORA Occur in or near Centromere Regions.
1986,
Pubmed
McGrew,
Single base-pair mutations in centromere element III cause aberrant chromosome segregation in Saccharomyces cerevisiae.
1986,
Pubmed
Mortimer,
Genetic map of Saccharomyces cerevisiae, edition 9.
1985,
Pubmed
Panzeri,
Role of conserved sequence elements in yeast centromere DNA.
1985,
Pubmed
Parry,
Radiation-induced mitotic and meiotic aneuploidy in the yeast Saccharomyces cerevisiae.
1979,
Pubmed
Prakash,
Characterization of postreplication repair in Saccharomyces cerevisiae and effects of rad6, rad18, rev3 and rad52 mutations.
1981,
Pubmed
Resnick,
Postreplication repair in Saccharomyces cerevisiae.
1981,
Pubmed
Resnick,
Repair of pyrimidine dimer damage induced in yeast by ultraviolet light.
1972,
Pubmed
Resnick,
Sunlight-induced killing in Saccharomyces cerevisiae.
1970,
Pubmed
Resnick,
Meiotic DNA metabolism in wild-type and excision-deficient yeast following UV exposure.
1983,
Pubmed
Resnick,
DNA polymerases, deoxyribonucleases, and recombination during meiosis in Saccharomyces cerevisiae.
1984,
Pubmed
Sedgwick,
Misrepair of overlapping daughter strand gaps as a possible mechanism for UV induced mutagenesis in UVR strains of Escherichia coli: a general model for induced mutagenesis by misrepair (SOS repair) of closely spaced DNA lesions.
1976,
Pubmed
Unrau,
Methods for the assay of ultraviolet light-induced pyrimidine dimers in Saccharomyces cerevisiae.
1972,
Pubmed
Wheatcroft,
Repair of UV-induced DNA damage and survival in yeast. I. Dimer excision.
1975,
Pubmed
Williams,
Deoxyribonucleic acid excision repair in chromatin after ultraviolet irradiation of human fibroblasts in culture.
1979,
Pubmed
Yeh,
Tightly centromere-linked gene (SPO15) essential for meiosis in the yeast Saccharomyces cerevisiae.
1986,
Pubmed
di Caprio,
DNA synthesis in UV-irradiated yeast.
1981,
Pubmed