XB-ART-32737
Biochim Biophys Acta
1975 Jun 16;3952:120-7.
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Isolation of a DNA fraction highly enriched in transfer RNA genes from Xenopus laevis.
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A DNA fraction highly enriched in tRNA genes can be isolated from the Xenopus laevis genome by the use of Ag+/Cs2SO4 density gradients. Ag+ shows a low affinity for some tRNA cistrons, allowing their separation from bulk DNA upon equilibrium centrifugation in a Cs2SO4 density gradient. Contaminating DNA in the resulting tDNA fraction is further removed by two additional CsCl density gradient centrifugations. The final DNA fraction is 60-fold enriched in tRNA genes, compared to the starting DNA material.
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Genes referenced: mt-tr trna