Cell Differ 1976 Mar 01;46:385-97. doi: 10.1016/0045-6039(76)90026-9.

Ribosomes from Xenopus laevis eggs and embryos in a cell-free protein-synthesizing system: translational regulation.

???displayArticle.abstract???
Three types of ribosomal preparations from Xenopus laevis eggs and embryos were tested in a cell-free system to study possible translational regulation of protein synthesis as mediated by the ribosome during early amphibian development: type 1, a crude high-speed sediment, mainly containing monoribosomes completely dissociable by 0.5 M KC1; type II, ribosomes washed with 0.5 M KC1; and type III, ribosomes treated with puromycin - 0.5 M KC1. All three types showed an active response to the addition of poly[U]. Type III was found to be the most active: levels of incorporation of 30 phenylalanine residues/ribosome were reached. In all three cases ribosomes prepared from unfertilized eggs were 30-40% less active in vitro than those from cleavage and gastrula stages.

???displayArticle.pubmedLink??? 944101
???displayArticle.link??? Cell Differ