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DNA supercoiling by Xenopus laevis oocyte extracts: requirement for a nuclear factor.
Baldi MI
,
Mattoccia E
,
Tocchini-Valentini GP
.
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A purified system is described for the introduction of negative supercoils into simian virus 40 DNA. The system consists of histones H2A, H2B, H3, and H4, DNA-relaxing enzyme, and a purified factor from Xenopus laevis stage 6oocyte nuclei. The nuclei are prepared en masse by the technique of F. Scalenghe, M. Buscaglia, C. Steinheil, and M. Crippa [(1978) Chromosoma 60, 299-308]. The supercoiled simian virus 40 DNA prepared by this method is indistinguishable from simian virus 40 supercoiled DNA prepared from infected monkey cells.
Adamson,
Histone synthesis in early amphibian development: histone and DNA syntheses are not co-ordinated.
1974, Pubmed,
Xenbase
Adamson,
Histone synthesis in early amphibian development: histone and DNA syntheses are not co-ordinated.
1974,
Pubmed
,
Xenbase
Bolivar,
Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
1977,
Pubmed
Destrée,
Analysis of histones from different tissues and embryos of Xenopus laevis (Daudin). II. Qualitative and quantitative aspects of nuclear histones during early stages of development.
1973,
Pubmed
,
Xenbase
Gellert,
DNA gyrase: an enzyme that introduces superhelical turns into DNA.
1976,
Pubmed
Gellert,
Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.
1977,
Pubmed
Germond,
Folding of the DNA double helix in chromatin-like structures from simian virus 40.
1975,
Pubmed
Hirt,
Selective extraction of polyoma DNA from infected mouse cell cultures.
1967,
Pubmed
Keller,
Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis.
1975,
Pubmed
Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase
Liu,
Micrococcus luteus DNA gyrase: active components and a model for its supercoiling of DNA.
1978,
Pubmed
Mattoccia,
DNA-relaxing activity and endonuclease activity in Xenopus laevis oocytes.
1976,
Pubmed
,
Xenbase
Scalenghe,
Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis.
1978,
Pubmed
,
Xenbase
Schorderet-Slatkine,
Action of progesterone and related steroids on oocyte maturation in Xenopus laevis. An in vitro study.
1972,
Pubmed
,
Xenbase
Sugino,
Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.
1977,
Pubmed
Tegtmeyer,
Simian virus 40 deoxyribonucleic acid synthesis: analysis by gel electrophoresis.
1972,
Pubmed
Woodland,
The synthesis and storage of histones during the oogenesis of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Wyllie,
Nuclear localisation of an oocyte component required for the stability of injected DNA.
1977,
Pubmed
,
Xenbase
