XB-ART-31913
Cell Differ
1978 Oct 01;75:259-70. doi: 10.1016/0045-6039(78)90027-1.
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Effects of estradiol on early messenger RNA in male Xenopus laevis liver.
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Cytoplasmic RNA isolated from male Xenopus liver between 1 and 6 h of hormone treatment was separated into distinct classes by Sepharose 4B chromatography. Assay of the mRNA activity of these RNA species in the wheat germ cell-free system demonstrated 2 populations of mRNA activity (peak 2 and peak 3). The activity of 1 population of mRNA (peak 2) was inducible by estradiol while the other (peak 3) remained unaltered for at least 6 h. mRNAs obtained by Sepharose 4B chromatography were also translated in the Xenopus oocyte. The translation products were analyzed by Sephadex chromatography and SDS-gel electrophoresis.
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