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Effects of estradiol-17beta in the male Xenopus laevis: isolation and translation of cytoplasmic messenger RNA populations.
Wiggins TL
,
Tucciarone LM
,
Lanclos KD
.
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Total Xenopus liver cytoplasmic RNA isolated following long-term estrogen administration (14 days) was fractioned using Sepharose 4B chromatography. One of the Sepharose 4B peaks was shown to contain RNA with a molecular weight reported for vitellogenin mRNA (approximately 34S). The presence of estrogen-induced vitellogenin mRNA in the peak 5 RNA was determined by translation of the RNA in the oocyte and analysis of the oocyte translational products by immunoprecipitation with anti-vitellogenin. Sepharose 4B peaks 2 and 3 were also observed to contain estrogen induced mRNA populations sedimenting between 9--18S. These findings suggest that Sepharose 4B chromatography might prove useful in separating different mRNA populations following estrogen-induced gene activation.
Berridge,
Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins.
1976, Pubmed,
Xenbase
Berridge,
Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins.
1976,
Pubmed
,
Xenbase
Clemens,
Sequential changes in the protein synthetic activity of male Xenopus laevis liver following induction of egg-yolk proteins by Estradiol-17 beta.
1975,
Pubmed
,
Xenbase
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Lanclos,
Translation of hormone-induced messenger RNA in amphibian oocytes: I. Induction by estrogen of messenger RNA encoded for vitellogenic protein in the liver of the male African clawed toad (Xenopus laevis).
1975,
Pubmed
,
Xenbase
O'Malley,
Female steroid hormones and target cell nuclei.
1974,
Pubmed
Palmiter,
Modulation of ovalbumin synthesis by estradiol-17 beta and actinomycin D as studied in explants of chick oviduct in culture.
1971,
Pubmed
STEELE,
EFFECTS OF THIOACETAMIDE ON THE COMPOSITION AND BIOSYNTHESIS OF NUCLEOLAR AND NUCLEAR RIBONUCLEIC ACID IN RAT LIVER.
1965,
Pubmed
Shapiro,
In vitro translation and estradiol-17beta induction of Xenopus laevis vitellogenin messenger RNA.
1976,
Pubmed
,
Xenbase
Tata,
The expression of the vitellogenin gene.
1976,
Pubmed
,
Xenbase
Wallace,
The induced synthesis and transport of yolk proteins and their accumulation by the oocyte in Xenopus laevis.
1968,
Pubmed
,
Xenbase
Woo,
Use of Sepharose 4B for preparative scale fractionation of eukaryotic messenger RNA's.
1974,
Pubmed
