Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Meunier-Rotival M
,
Cortadas J
,
Macaya G
,
Bernardi G
.
???displayArticle.abstract???
Ribosomal DNA (rDNA) from calf was isolated by three density gradient centrifugations. The first centrifugation in Cs2S04/BAMD was used to obtain partially resolved dG+dC-rich fractions from total DNA. The second and third centrifugations, in Cs2S04/Ag+, led to the isolation of an rDNA fraction characterized by a symmetrical band in CsCl, p = 1.724 g/cm3. This new procedure appears to be generally suitable for the isolation of rDNA and other dG+dC-rich repeated genes. The organization of isolated calf rDNA has been studied by restriction enzyme digestion and by hybridization with cloned rDNA from Xenopus laevis. The repeat unit of calf rDNA has a molecular weight of 21x10(6) and is split by EcoR1 into two fragments, 16x10(6) and 5.0x10(6), and by BamHI into seven fragments. EcoRI and BamHI sites have been mapped. Most of the 18S and 28S RNA genes and the transcribed spacer are contained in the small EcoRI fragment, while the non-transcribed spacer is localized in the large EcoRI fragment. This spacer showed length heterogeneity within a single individual; such heterogeneity is limited to two regions of the spacer.
Arnheim,
Heterogeneity of the ribosomal genes in mice and men.
1977, Pubmed
Arnheim,
Heterogeneity of the ribosomal genes in mice and men.
1977,
Pubmed
Bachmann,
Genome size in mammals.
1972,
Pubmed
Birnstiel,
The ribosomal RNA cistrons.
1971,
Pubmed
Blin,
Isolation and some properties of a mammalian ribosomal DNA.
1976,
Pubmed
Botchan,
Restriction analysis of the nontranscribed spacers of Xenopus laevis ribosomal DNA.
1977,
Pubmed
,
Xenbase
Buongiorno-Nardelli,
Size of ribosomal DNA repeating units in Xenopus laevis: limited individual heterogeneity and extensive population polymorphism.
1977,
Pubmed
,
Xenbase
Cortadas,
An analysis of the bovine genome by density gradient centrifugation: fractionation in Cs2SO4/3,6-bis(acetatomercurimethyl)dioxane density gradient.
1977,
Pubmed
Cory,
A very large repeating unit of mouse DNA containing the 18S, 28S and 5.8S rRNA genes.
1977,
Pubmed
Denhardt,
A membrane-filter technique for the detection of complementary DNA.
1966,
Pubmed
Filipski,
An analysis of the bovine genome by Cs2SO4-Ag density gradient centrifugation.
1973,
Pubmed
Gillespie,
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
1965,
Pubmed
Lebowitz,
Hemophilus aegyptius restriction edonuclease cleavage map of the simian virus 40 genome and its colinear relation with the hemophilus influenzae cleavage map of SV40.
1974,
Pubmed
Macaya,
An analysis of the bovine genome by density-gradient centrifugation. Preparation of the dG+dC-rich DNA components.
1978,
Pubmed
Melli,
Clustering of the DNA sequences complementary to repetitive nuclear RNA of HeLa cells.
1975,
Pubmed
Morrow,
Replication and transcription of eukaryotic DNA in Escherichia coli.
1974,
Pubmed
,
Xenbase
Polisky,
Specificity of substrate recognition by the EcoRI restriction endonuclease.
1975,
Pubmed
Prunell,
The mitochondrial genome of wild-type yeast cells. V. Genome evolution.
1977,
Pubmed
Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
Pubmed
Sinclair,
Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of eukaryotes and some of their physical characteristics.
1971,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Southern,
Long range periodicities in mouse satellite DNA.
1975,
Pubmed
Thomas,
Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
1975,
Pubmed
Tiemeier,
Purification and cloning of a mouse ribosomal gene fragment in coliphage lambda.
1977,
Pubmed
Wellauer,
The molecular basis for length heterogeneity in ribosomal DNA from Xenopus laevis.
1976,
Pubmed
,
Xenbase
Wellauer,
A comparison of the structural organization of amplified ribosomal DNA from Xenopus mulleri and Xenopus laevis.
1975,
Pubmed
,
Xenbase
