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XB-ART-31674
Somatic Cell Genet 1979 Jul 01;54:529-36. doi: 10.1007/bf01538886.
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Effects of pH, Ca2+, temperature, and protease pretreatment on interkingdom fusion.

Ward M , Davey MR , Mathias RJ , Cocking EC , Clothier RH , Balls M , Lucy JA .


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The incubation of carrot protoplasts and cultured Xenopus cells in a protease solution has been shown to enhance their subsequent interkingdom fusion by a high pH/high Ca2+ method. The effects of Ca2+ concentration, pH, and temperature on the frequency of heterokaryon formation have also been studied. Potentially viable heterokaryons have been repeatedly produced at high frequencies (consistently greater than 10%), far exceeding those so far achieved in PEG-mediated fusion. Cell aggregates are readily dispersed after this method of fusion, permitting the accurate estimation of fusion frequencies.

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