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XB-ART-31583
Cell 1979 Nov 01;183:643-8.
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Separation of RNA transcription and processing activities from X. laevis germinal vesicles.

Mattoccia E , Baldi MI , Carrara G , Fruscoloni P , Benedetti P , Tocchini-Valentini GP .


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A procedure suitable for en masse preparation of germinal vesicles (GV) from X.laevis oocytes (Scalenghe et al., 1978) has been adapted for studies of transcription. Extracts from GV contain activities for transcription of tRNA genes and for processing the transcription product. The two activities have been separated by column chromatography. One fraction allows synthesis of tRNA precursor molecules in the presence of X.laevis RNA polymerase III. Another fraction contains the activity that cuts and splices those precursors which contain an intervening sequence. Transcription occurs faithfully on linear DNA fragments.

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Species referenced: Xenopus
Genes referenced: mt-tr tbx2 trna