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Translation of globin messenger RNA by the mouse ovum.
Brinster RL
,
Chen HY
,
Trumbauer ME
,
Avarbock MR
.
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It has been demonstrated that the Xenopus oocyte can translate rabbit haemoglobin messenger RNA (mRNA) following microinjection of the message into the cell. The Xenopus oocyte has since been shown to be capable of translating a variety of messenger RNAs from different species. This system has proved useful in un-erstanding the mechanism of message translation and has also provided information about the translation capability of the Xenopus oocyte. Several other cell types, including HeLa cells and fibroblasts, can also translate exogenous message injected into the cell. However, there have been no reports of injection of mRNA into oocytes or fertilised one-cell ova of mammalian species. Nevertheless, the latter system could be of considerable use in studying the processing of exogenous messages in a mammalian system undergoing development, as well as providing insight into the way the early embryo processes injected messages and the protein products of such messages. We report here the results of injecting message into the fertilised one-cell mouse ovum and show that both mouse and rabbit globin mRNA are translated in this system.
Asselbergs,
Messenger RNA competition in living Xenopus oocytes.
1979, Pubmed,
Xenbase
Asselbergs,
Messenger RNA competition in living Xenopus oocytes.
1979,
Pubmed
,
Xenbase
Brinster,
Protein degradation in the mouse blastocyst.
1979,
Pubmed
Brinster,
The effect of cells transferred into the mouse blastocyst on subsequent development.
1974,
Pubmed
Colman,
Export of proteins from oocytes of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Gorski,
Size heterogeneity of polyadenylate sequences in mouse globin messenger RNA.
1974,
Pubmed
Gurdon,
Message stability in injected frog oocytes: long life of mammalian alpha and beta globin messages.
1973,
Pubmed
,
Xenbase
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Krystosek,
Improved methods for purification and assay of eukaryotic messenger ribonucleic acids and ribosomes. Quantitative analysis of their interaction in a fractionated reticulocyte cell-free system.
1975,
Pubmed
Laskey,
Protein synthesis in oocytes of Xenopus laevis is not regulated by the supply of messenger RNA.
1977,
Pubmed
,
Xenbase
Mous,
Translation of biologically active messenger RNA from human placenta in Xenopus oocytes.
1979,
Pubmed
,
Xenbase
O'Farrell,
High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
1977,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
Stacey,
Microinjection studies of duck globin messenger RNA translation in human and avian cells.
1976,
Pubmed
Stacey,
Microinjection analysis of envelope-glycoprotein messenger activities of avian leukosis viral RNAs.
1977,
Pubmed