Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The messenger RNA sequences in human fibroblast cells induced with poly rI.rC to produce interferon.
Raj NB
,
Pitha PM
.
???displayArticle.abstract???
Induction of human fibroblast cells with poly rI.rC induces interferon mRNA which can be translated into interferon precursor in wheat germ cell free system or in Xenopus oocytes into biologically active interferon. The extent of gene expression in the poly rI.rC induced cells was compared to that of the uninduced cells by hybridization of the mRNA to complementary DNA. Homologous template driven hybridization of cDNA revealed the presence of two clearly defined transitions in the total poly A RNA from the induced cells; abundant class and a scarce class comprising approximately 37,000 diverse species of RNA. Heterologus hybridization of the cDNA with total uninduced mRNA showed that the majority of the mRNA sequences are the same in both the induced and uninduced cells. The results of the hybridization using cDNA prepared to the fraction enriched for interferon mRNA, however, showed that about 4% of the sequences present in the interferon enriched fraction are not present in the uninduced cells. These differences may result from the poly rI.rC induced alterations in gene expression.
Bishop,
Polynucleotide sequences in eukaryotic DNA and RNA that form ribonuclease-resistant complexes with polyuridylic acid.
1974, Pubmed
Bishop,
Polynucleotide sequences in eukaryotic DNA and RNA that form ribonuclease-resistant complexes with polyuridylic acid.
1974,
Pubmed
Bishop,
Three abundance classes in HeLa cell messenger RNA.
1974,
Pubmed
Buell,
Synthesis of full length cDNAs from four partially purified oviduct mRNAs.
1978,
Pubmed
Cavalieri,
Induction and decay of human fibroblast interferon mRNA.
1977,
Pubmed
,
Xenbase
De Maeyer-Guignard,
Purification of mouse interferon by sequential affinity chromatography on poly(U)--and antibody--agarose columns.
1978,
Pubmed
Efstratiadis,
Full length and discrete partial reverse transcripts of globin and chorion mRNAs.
1975,
Pubmed
Greene,
Inactivation of interferon mRNA in the shutoff of human interferon synthesis.
1978,
Pubmed
Hastie,
The expression of three abundance classes of messenger RNA in mouse tissues.
1976,
Pubmed
Kacian,
Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.
1976,
Pubmed
Lebleu,
Translation of mouse interferon mRNA in Xenopus laevis oocytes and in rabbit reticulocyte lysates.
1978,
Pubmed
,
Xenbase
Liu,
Biological detection of specific mRNA molecules by microinjection.
1979,
Pubmed
Monahan,
The synthesis and properties of the complete complementary DNA transcript of ovalbumin mRNA.
1976,
Pubmed
Raj,
Relationship between interferon production and interferon messenger RNA synthesis in human fibroblasts.
1977,
Pubmed
,
Xenbase
Reynolds,
Interferon activity produced by translation of human interferon messenger RNA in cell-free ribosomal systems and in Xenopus oöcytes.
1975,
Pubmed
,
Xenbase
Rubinstein,
Human leukocyte interferon: production, purification to homogeneity, and initial characterization.
1979,
Pubmed
Sehgal,
Superinduction of human fibroblast interferon production: further evidence for increased stability of interferon mRNA.
1978,
Pubmed
Shapiro,
Purification and characterization of Xenopus laevis vitellogenin messenger RNA.
1977,
Pubmed
,
Xenbase
Strohman,
Messenger RNA for myosin polypeptides: isolation from single myogenic cell cultures.
1977,
Pubmed
Thang,
Biosynthesis of mouse interferon by translation of its messenger RNA in a cell-free system.
1975,
Pubmed
Williams,
The messenger RNA sequences in growing and resting mouse fibroblasts.
1975,
Pubmed
