Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-31270
Nucleic Acids Res 1980 Nov 25;822:5169-78.
Show Gene links Show Anatomy links

Nonsense suppression in eukaryotes: the use of the Xenopus oocyte as an in vivo assay system.

Bienz M , Kubli E , Kohli J , de Henau S , Grosjean H .


???displayArticle.abstract???
Amber, ochre, and opal nonsense suppressor tRNAs isolated from yeast were injected into Xenopus laevis oocytes together with purified mRNAs (globin mRNA from rabbit, tobacco mosaic virus-RNA). Yeast opal suppressor tRNA is able to read the UGA stop codon of the rabbit beta-globin mRNA, thus producing a readthrough protein. A large readthrough product is also obtained upon coinjection of yeast amber or ochre suppressor tRNA with TMV-RNA. The amount of readthrough product is dependent on the amount of injected suppressor tRNA. The suppression of the terminator codon of TMV-RNA is not susceptible to Mg++ concentration or polyamine addition. Therefore, the Xenopus laevis oocyte provides a simple, sensitive, and well buffered in vivo screening system for all three types of eukaryotic nonsense suppressor tRNAs.

???displayArticle.pubmedLink??? 7465411
???displayArticle.pmcLink??? PMC324292
???displayArticle.link??? Nucleic Acids Res


Species referenced: Xenopus laevis
Genes referenced: hbg1 mt-tr trna

References [+] :
Aoi, Read-through proteins of group 4 RNA bacteriophages TW19 and TW28. 1976, Pubmed