Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
tRNA synthesis: identification of in vivo precursor tRNAs from parental and mutant yeast strains.
Hopper AK
,
Kurjan J
.
???displayArticle.abstract???
In vivo yeast precursor tRNAs have been identified using a modification of the Northern-hybridization procedure. Two species of pre-tRNA Tyr, 1 species of pre-tRNA Ser2 and 2 species of pre-tRNA Serminor have been found in all yeast strains examined, including parental strains and strains harboring mutations affecting tRNA function. One of the tRNA Tyr strains harboring and one of the pre-tRNA SerUCG are the same size as the unspliced pre-tRNAs which accumulate in the yeast mutant rna1. The in vivo tRNA Tyr precursors detected in these studies also appear similar with the RNA species identified when cloned yeast tRNA Tyr is transcribed and processed by Xenopus oocytes and/or Xenopus extracts. We have also studied the precursor and mature tRNA Tyr species from 22 mutants which contain mutations in the SUP4 tyrosine-inserting suppressor locus. The RNA from 2 mutants mapping at the G52 position showing an aberrantly migrating "mature" tRNA Tyr. Although several of those cloned mutant genes showed transcript products of altered size in in vitro transcription studies (1), we did not detect such altered transcripts in vivo.
Alwine,
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
1977, Pubmed
Alwine,
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
1977,
Pubmed
Alwine,
Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.
1979,
Pubmed
De Robertis,
Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytes.
1979,
Pubmed
,
Xenbase
Etcheverry,
A precursor to a minor species of yeast tRNASer contains an intervening sequence.
1979,
Pubmed
Garber,
Transcription of a cloned Bombyx mori tRNA2Ala gene: nucleotide sequence of the tRNA precursor and its processing in vitro.
1979,
Pubmed
Goodman,
Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.
1977,
Pubmed
HOLLEY,
A simplified procedure for the preparation of tyrosine and valine-acceptor fractions of yeast "soluble ribonucleic acid".
1961,
Pubmed
Hagenbüchle,
The primary transcription product of a silkworm alanine tRNA gene: identification of in vitro sites of initiation, termination and processing.
1979,
Pubmed
,
Xenbase
Hopper,
A yeast mutant which accumulates precursor tRNAs.
1978,
Pubmed
Hopper,
Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs.
1980,
Pubmed
Hutchison,
Temperature-sensitive yeast mutant defective in ribonucleic acid production.
1969,
Pubmed
Knapp,
Transcription and processing of intervening sequences in yeast tRNA genes.
1978,
Pubmed
Knapp,
Splicing of yeast tRNA precursors: structure of the reaction intermediates.
1979,
Pubmed
Koski,
Mutations of the yeast SUP4 tRNATyr locus: transcription of the mutant genes in vitro.
1980,
Pubmed
,
Xenbase
Kurjan,
Mutations at the yeast SUP4 tRNATyr locus: DNA sequence changes in mutants lacking suppressor activity.
1980,
Pubmed
Madison,
Large oligonucleotides isolated from yeast tyrosine transfer ribonucleic acid after partial digestion with ribonuclease T1.
1967,
Pubmed
Melton,
Order and intracellular location of the events involved in the maturation of a spliced tRNA.
1980,
Pubmed
,
Xenbase
Miyazaki,
Studies on the nucleotide sequence of pseudouridine-containing 5S RNA from Saccharomyces cerevisiae.
1974,
Pubmed
Mortimer,
Genetic mapping in Saccharomyces.
1966,
Pubmed
O'Farrell,
Structure and processing of yeast precursor tRNAs containing intervening sequences.
1978,
Pubmed
Olson,
Cloning of the yeast tyrosine transfer RNA genes in bacteriophage lambda.
1979,
Pubmed
Olson,
Molecular characterisation of the tyrosine tRNA genes of yeast.
1977,
Pubmed
Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
Pubmed
Rubin,
The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid.
1973,
Pubmed
Silverman,
The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts.
1979,
Pubmed
,
Xenbase
Sprague,
5' flanking sequence signals are required for activity of silkworm alanine tRNA genes in homologous in vitro transcription systems.
1980,
Pubmed
,
Xenbase
Stellwag,
Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl (DBM) - paper.
1980,
Pubmed
Valenzuela,
Structure of yeast phenylalanine-tRNA genes: an intervening DNA segment within the region coding for the tRNA.
1978,
Pubmed
Wahl,
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
1979,
Pubmed
Zachau,
The structures of two serine transfer ribonucleic acids.
1966,
Pubmed
