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Construction of recombinant plasmids containing Xenopus immunoglobulin heavy chain DNA sequences.
Brown RD
,
Armentrout RW
,
Cochran MD
,
Cappello J
,
Langemeier SO
.
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A recombinant cDNA plasmid containing Xenopus immunoglobulin heavy chain sequence has been constructed from Xenopus spleen poly(A)-containing RNA. The plasmid was identified by colony hybridization and a hybridization-translation assay and its identity was confirmed by DNA sequence analysis. The portion of the heavy chain sequence contained in the plasmid is 35% homologous to mammalian mu and gamma sequences. The mRNA corresponding to this plasmid is 2.5 kilobases, in close agreement with the size of mouse mu mRNA. RNA sequences complementary to the cloned sequence appear in embryos about 24 hr after fertilization, which corresponds to 24 hr before the first detectable immunoglobulin.
Alt,
Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3' ends.
1980, Pubmed
Alt,
Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3' ends.
1980,
Pubmed
Alwine,
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
1977,
Pubmed
Alwine,
Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.
1979,
Pubmed
Bailey,
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
1976,
Pubmed
Breathnach,
Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.
1978,
Pubmed
Cochran,
The identification of messenger RNA coding for immunoglobulin heavy and light chains of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Cory,
Deletions are associated with somatic rearrangement of immunoglobulin heavy chain genes.
1980,
Pubmed
Davis,
An immunoglobulin heavy-chain gene is formed by at least two recombinational events.
1980,
Pubmed
Donelson,
Nucleotide sequence analysis of deoxyribonucleic acid. VI. Determination of 3'-terminal dnucleotide sequences of several species of duplex deoxyribonucleic acid using Escherichia coli deoxyribonucleic acid polymerase I.
1972,
Pubmed
Du Pasquier,
The ontogenesis of lymphocyte diversity in anuran amphibians.
1977,
Pubmed
Du Pasquier,
Direct evidence for immunoglobulins on the surface of thymus lymphocytes of amphibian larvae.
1972,
Pubmed
Grunstein,
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
1975,
Pubmed
Hadji-Azimi,
Studies on Xenopus laevis immunoglobulins.
1971,
Pubmed
,
Xenbase
Hadji-Azimi,
Xenopus laevis 19S immunoglobulin. Ultrastructure and J chain isolation.
1976,
Pubmed
,
Xenbase
Honjo,
Organization of immunoglobulin heavy chain genes and allelic deletion model.
1978,
Pubmed
Kehry,
Amino acid sequence of a mouse immunoglobulin mu chain.
1979,
Pubmed
Kobayashi,
Identification of J-chain in polymeric immunoglobulins from a variety of species by cross-reaction with rabbit antisera to human J-chain.
1973,
Pubmed
Leverone,
Characteristics of immunoglobulin synthesized early in the development of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
McCumber,
The complete amino-acid sequence of a canine mu chain.
1979,
Pubmed
Moyer,
The onset of immunoglobulin synthesis during the development of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Perry,
Immunoglobulin messenger RNAs in murine cell lines that have characteristics of immature B lymphocytes.
1979,
Pubmed
Proudfoot,
3' non-coding region sequences in eukaryotic messenger RNA.
1976,
Pubmed
Rabbitts,
The role of gene deletion in the immunoglobulin heavy chain switch.
1980,
Pubmed
Richardson,
Partial sequence analysis of Xenopus alpha- and beta-globin mRNA as determined from recombinant DNA plasmids.
1980,
Pubmed
,
Xenbase
Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
Pubmed
Rogers,
Two mRNAs with different 3' ends encode membrane-bound and secreted forms of immunoglobulin mu chain.
1980,
Pubmed
Villa-Komaroff,
A bacterial clone synthesizing proinsulin.
1978,
Pubmed
Weinheimer,
Species distribution of J chain.
1971,
Pubmed
Zain,
Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA.
1979,
Pubmed