Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-30851
J Cell Biochem 1982 Jan 01;184:507-13. doi: 10.1002/jcb.1982.240180411.
Show Gene links Show Anatomy links

Benzo(a)pyrene-binding proteins of hamster embryo cell nuclei: comparison of nuclear isolation procedures.

MacLeod MC , Mansfield BK , Selkirk JK .


???displayArticle.abstract???
Hamster embryo cells metabolize benzo(a)pyrene to derivatives that covalently modify the nuclear macromolecules including proteins. Not all proteins are modified to the same extent nor by the same metabolites. In particular, a protein of apparent molecular weight 32,000 is highly modified by derivatives of trans-9,10-dihydro-9,10-dihydroxy B(a)P. This protein is shown here to be preferentially lost from nuclei during purification by centrifugation through high molarity sucrose solutions followed by osmotic shock. It does not appear to be a cytoplasmic contaminant, but shares many properties of an abundant protein from Xenopus laevis oocytes, nucleoplasmin.

???displayArticle.pubmedLink??? 6282910
???displayArticle.link??? J Cell Biochem


Species referenced: Xenopus laevis
Genes referenced: npm1