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XB-ART-30825
Arch Anat Microsc Morphol Exp 1982 Jan 01;712:89-98.
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[Detection, using fluorescent lectins, of specific carbohydrate chains in primordial germ cells of anuran amphibians].

Delbos M , Saidi N , Gipouloux JD .


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A study of primordial germ cells (PGC) of Amphibia Anura was carried out after treatment of sections by different fluorescein isothiocyanate conjugated lectins (FITC-lectins). Specific labelling on the PGC is obtained with lectins, the activity of which is inhibited by D-galactose or N-acetyl-galactosamine. These osidic groups appear to be located more specifically on the PGC. The same labelling pattern is not obtained with lectins possessing major affinity for mannose, glucose, fucose and N-acetyl-glucosamine. Furthermore, changes in labelling pattern are observed during migration of PGC. It is suggested that D-galactose and N-acetyl-galactosamine might be related to membrane activity of PGC during migration. Ultrastructural study of the visualization of cell surface carbohydrates supplies some information on the localisation of these lectins receptors.

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Species referenced: Xenopus
Genes referenced: pgc