Comp Biochem Physiol B 1982 Jan 01;734:839-43.

Cellular DNA content in different species of Xenopus.

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1. Biochemical methods were applied to determine the DNA content per cell in ten species and subspecies of Xenopus. 2. Cell density and DNA concentration were measured in a suspension of blood cells. 3. DNA was determined by the diphenylamine method and values were compared with those obtained by the ultraviolet absorption method and by cytofluorometry. 4. A good degree of reproducibility was demonstrated by a simple biochemical method which yielded values compatible with cytofluorometry. 5. Results suggested that the DNA of Xenopus muelleri, and perhaps that of other species with a large genome size, may be specially protected from chemical degradation.

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