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Cloning and determination of the transcription termination site of ribosomal RNA gene of the mouse.
Kominami R
,
Mishima Y
,
Urano Y
,
Sakai M
,
Muramatsu M
.
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A Eco RI 6.6 kb DNA fragment containing the 3'-end of 28S ribosomal RNA gene of the mouse was detected by Southern blot hybridization, and cloned in a lambda-phage vector. The site of transcription termination and the processed 3'-end of 28S RNA were determined on the cloned fragment and the surrounding nucleotide sequence determined. The 3'-terminal nucleotides of mouse 28S RNA are similar to those of yeast, Drosophila and Xenopus although the homology was lost drastically beyond the 3'-end of 28S RNA. 45S precursor RNA terminated at 30 nucleotides downstream from the 3'-end of 28S RNA gene. A structure of a dyad symmetry with a loop was found immediately prior to the termination site of 45S RNA. The rDNA termination site thus shares some common features with termination sites recognized by other RNA polymerases.
Arnheim,
Heterogeneity of the ribosomal genes in mice and men.
1977, Pubmed
Arnheim,
Heterogeneity of the ribosomal genes in mice and men.
1977,
Pubmed
Arnheim,
Characterization of mouse ribosomal gene fragments purified by molecular cloning.
1979,
Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Bertrand,
The attenuator of the tryptophan operon of Escherichia coli. Heterogeneous 3'-OH termini in vivo and deletion mapping of functions.
1977,
Pubmed
Blattner,
Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections.
1978,
Pubmed
Bruce,
Reactions at the termini of tRNA with T4 RNA ligase.
1978,
Pubmed
Celma,
Studies of low molecular weight RNA from cells infected with adenovirus 2. I. The sequences at the 3' end of VA-RNA I.
1977,
Pubmed
Cory,
A very large repeating unit of mouse DNA containing the 18S, 28S and 5.8S rRNA genes.
1977,
Pubmed
Enea,
Genetic studies with heteroduplex DNA of bacteriophage fl. Asymmetric segregation, base correction and implications for the mechanism of genetic recombination.
1975,
Pubmed
Gross-Bellard,
Isolation of high-molecular-weight DNA from mammalian cells.
1973,
Pubmed
Grummt,
Characterization of a cloned ribosomal fragment from mouse which contains the 18S coding region and adjacent spacer sequences.
1979,
Pubmed
Hamada,
3'-terminal processing of ribosomal RNA precursors in mammalian cells.
1980,
Pubmed
Hentschel,
Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences.
1980,
Pubmed
Kominami,
Heterogeneity of 5' -termini of nucleolar 45S, 32S and 28S RNA in mouse hepatoma.
1977,
Pubmed
Kominami,
5'-Terminal processing of ribosomal 28S RNA.
1978,
Pubmed
Korn,
Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.
1978,
Pubmed
,
Xenbase
Mandal,
The nucleotide sequence at the transcription termination site of ribosomal RNA in Drosophila melanogaster.
1981,
Pubmed
,
Xenbase
Maniatis,
Nucleotide sequence of the rightward operator of phage lambda.
1975,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
Mishima,
Cloning and determination of a putative promoter region of a mouse ribosomal deoxyribonucleic acid fragment.
1980,
Pubmed
Perry,
Processing of RNA.
1976,
Pubmed
Rosenberg,
The relationship between function and DNA sequence in an intercistronic regulatory region in phage lambda.
1978,
Pubmed
Sollner-Webb,
The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
1979,
Pubmed
,
Xenbase
Southern,
Long range periodicities in mouse satellite DNA.
1975,
Pubmed
Tabak,
A method for the recovery of DNA from agarose gels.
1978,
Pubmed
Thomas,
Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA.
1974,
Pubmed
Thomas,
Hybridization of RNA to double-stranded DNA: formation of R-loops.
1976,
Pubmed
Tiemeier,
Purification and cloning of a mouse ribosomal gene fragment in coliphage lambda.
1977,
Pubmed
Urano,
The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.
1980,
Pubmed
,
Xenbase
Valenzuela,
Ribosomal RNA genes of Saccharomyces cerevisiae. II. Physical map and nucleotide sequence of the 5 S ribosomal RNA gene and adjacent intergenic regions.
1977,
Pubmed
Veldman,
The transcription termination site of the ribosomal RNA operon in yeast.
1980,
Pubmed
,
Xenbase
Wellauer,
Secondary structure maps of ribosomal RNA. II. Processing of mouse L-cell ribosomal RNA and variations in the processing pathway.
1974,
Pubmed
Wilson,
Molecular analysis of cloned human 18S ribosomal DNA segments.
1978,
Pubmed
Wu,
Transcription termination: nucleotide sequence at 3' end of tryptophan operon in Escherichia coli.
1978,
Pubmed
Young,
Transcription termination in the Escherichia coli ribosomal RNA operon rrnC.
1979,
Pubmed