J Cell Physiol 1982 Jun 01;1113:284-90. doi: 10.1002/jcp.1041110309.

Parallel changes in protein synthesis and messenger RNA content in growing and resting epithelial cells of Xenopus laevis.

???displayArticle.abstract???
The regulation of cell growth can be achieved at many levels but ultimately the regulatory factors must alter protein synthesis since growing cells always exhibit an increased rate of protein synthesis compared to resting cells. Some studies using growing and nongrowing mammalian cells have shown that the rate of protein synthesis is directly dependent on mRNA content. Other studies have shown that growing the resting cells have similar amounts of mRNA and that protein synthesis is regulated by the proportion of mRNA in polysomes. We have analyzed mRNA content in growing and resting epithelial cells of Xenopus laevis. Quantitation of poly(A)+ mRNA by uniform labeling with 3H-uridine and by 3H-poly(U) hybridization demonstrated a direct relationship between mRNA content and the relative rate of protein synthesis in growing and resting cells. Likewise, after serum stimulation of resting cells the increase in mRNA content closely paralleled the increase in protein synthesis. Our results suggest that control of protein synthesis in growing and nongrowing cells is exerted before the translational level.

???displayArticle.pubmedLink??? 6178747
???displayArticle.link??? J Cell Physiol