Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Virol
1982 Aug 01;432:386-94. doi: 10.1128/JVI.43.2.386-394.1982.
Show Gene links
Show Anatomy links
mRNA- and DNA-directed synthesis of herpes simplex virus-coded exonuclease in Xenopus laevis oocytes.
Preston CM
,
Cordingley MG
.
???displayArticle.abstract???
Microinjection of herpes simplex virus (HSV)-infected cell mRNA into Xenopus laevis oocytes resulted in the production of a new exonuclease activity. This enzyme strongly resembled the HSV alkaline exonuclease in many biochemical properties, and hybrid-arrested translation studies showed that it was virus coded, mapping at 0.080 to 0.185 genome map units. Exonuclease mRNA had a size and genome location equivalent to the mRNA encoding V185 in reticulocyte lysates, suggesting that V185 is the exonuclease. The enzyme synthesized in oocytes was found to act as an exonuclease in vivo. Two plasmids containing HSV DNA fragments directed the synthesis of exonuclease when microinjected into oocyte nuclei, and this finding enabled the coding and control sequences for this gene to be localized to 0.155 to 0.185 genome map units.
Anderson,
Isolation and translation of mRNA encoded by a specific region of the herpes simplex virus type 1 genome.
1980, Pubmed
Anderson,
Isolation and translation of mRNA encoded by a specific region of the herpes simplex virus type 1 genome.
1980,
Pubmed
Chartrand,
Mutant of herpes simplex virus type 2 with temperature-sensitive lesions affecting virion thermostability and DNase activity: identification of the lethal mutation and physical mapping of the nuc-lesion.
1979,
Pubmed
Clements,
Analysis of herpesvirus DNA substructure by means of restriction endonucleases.
1976,
Pubmed
Cordingley,
Transcription and translation of the herpes simplex virus type 1 thymidine kinase gene after microinjection into Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase
Costa,
Characterization of a major late herpes simplex virus type 1 mRNA.
1981,
Pubmed
Cremer,
Characterization of the mRNA for herpes simplex virus thymidine kinase by cell-free synthesis of active enzyme.
1978,
Pubmed
Docherty,
Identification of a virus-specific polypeptide associated with a transforming fragment (BglII-N) of herpes simplex virus type 2 DNA.
1981,
Pubmed
Francke,
Alkaline DNase activity in cells infected with a temperature-sensitive mutant of herpes simplex virus type 2.
1978,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Hoffmann,
The deoxyribonuclease induced after infection of KB cells by herpes simplex virus type 1 or type 2. I. Purification and characterization of the enzyme.
1978,
Pubmed
Hoffmann,
Mechanism of degradation of duplex DNA by the DNase induced by herpes simplex virus.
1981,
Pubmed
Holland,
Isolation and localization of herpes simplex virus type 1 mRNA abundant before viral DNA synthesis.
1979,
Pubmed
Honess,
Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins.
1974,
Pubmed
Jamieson,
Biochemical studies on the herpes simplex virus-specified deoxypyrimidine kinase activity.
1974,
Pubmed
Kit,
Regulation of herpesvirus thymidine kinase activity in LM(TK) cells transformed by ultraviolet light-irradiated herpes simplex virus.
1977,
Pubmed
Knopf,
Properties of herpes simplex virus DNA polymerase and characterization of its associated exonuclease activity.
1979,
Pubmed
Leiden,
Herpes simplex virus gene expression in transformed cells. I. Regulation of the viral thymidine kinase gene in transformed L cells by products of superinfecting virus.
1976,
Pubmed
Marsden,
Physical mapping of herpes simplex virus-induced polypeptides.
1978,
Pubmed
McKnight,
Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro.
1980,
Pubmed
,
Xenbase
McKnight,
Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: identification of an upstream control region.
1981,
Pubmed
,
Xenbase
Morrison,
A new DNA-exonuclease in cells infected with herpes virus: partial purification and properties of the enzyme.
1968,
Pubmed
Morse,
Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 X HSV-2 recombinants.
1978,
Pubmed
Preston,
The cell-free synthesis of herpesvirus-induced polypeptides.
1977,
Pubmed
Preston,
Cell-free synthesis of herpes simplex virus-coded pyrimidine deoxyribonucleoside kinase enzyme.
1977,
Pubmed
Preston,
Control of herpes simplex virus type 1 mRNA synthesis in cells infected with wild-type virus or the temperature-sensitive mutant tsK.
1979,
Pubmed
Preston,
Identification and mapping of two polypeptides encoded within the herpes simplex virus type 1 thymidine kinase gene sequences.
1981,
Pubmed
Strobel-Fidler,
Alkaline deoxyribonuclease induced by herpes simplex virus type 1: composition and properties of the purified enzyme.
1980,
Pubmed
Summers,
[125I]deoxycytidine used in a rapid, sensitive, and specific assay for herpes simplex virus type 1 thymidine kinase.
1977,
Pubmed
Watson,
Separation and characterization of herpes simplex virus type 1 immediate-early mRNA's.
1979,
Pubmed
Wilcox,
Herpes simplex virus phosphoproteins. I. Phosphate cycles on and off some viral polypeptides and can alter their affinity for DNA.
1980,
Pubmed