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The action of puromycin and cycloheximide on the initiation of rapid axonal transport in amphibian dorsal root neurones.
Nichols TR
,
Smith RS
,
Snyder RE
.
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1. Amphibian dorsal root ganglia-sciatic nerve preparations were incubated in vitro and the rapid axonal transport of radioactive labels was studied with a position-sensitive detector and by conventional liquid scintillation analysis. Protein was labelled by exposure of the ganglia to [(35)S]methionine or [(3)H]leucine and lipid was labelled using [(32)P]orthophosphoric acid.2. Protein synthesis was interrupted by exposure of the ganglia to either cycloheximide or puromycin. When ganglia were exposed to either inhibitor prior to or simultaneously with a label, the somal export of both protein and lipid to the axon was reduced by two to three orders of magnitude.3. Using the position-sensitive detector, [(35)S]methionine was observed to be exported from the ninth dorsal root ganglia of Rana catesbiana 3.49+/-1.56 h (+/- S.D.) after exposure, and [(32)P]phosphate 4.46+/-1.85 h after exposure.4. Export of [(35)S]methionine or [(32)P]phosphate was disrupted 3.32+/-1.21 h (+/- S.D.) or 1.93+/-1.04 h respectively after exposure of the ganglia to cycloheximide or puromycin.5. For a given preparation the time required for [(35)S]methionine to be exported was statistically equal to the time required for cycloheximide or puromycin to disrupt export. No such correlation was found to exist for the export of [(32)P]phosphate.6. Analysis revealed that materials labelled with either [(35)S]methionine or [(32)P]phosphate continue to be exported from the ganglia for several hours after the initial disruption in outflow caused by the inhibitors.7. The results do not provide support for the hypothesis of Ambron, Goldman & Schwartz (1975) that a ;key' newly synthesized, and non-storable, polypeptide is added to an already assembled structure to allow rapid axonal transport to be initiated.
Abe,
Rapid transport of phosphatidylcholine occurring simultaneously with protein transport in the frog sciatic nerve.
1973, Pubmed
Abe,
Rapid transport of phosphatidylcholine occurring simultaneously with protein transport in the frog sciatic nerve.
1973,
Pubmed
Ambron,
Effect of inhibiting protein synthesis on axonal transport of membrane glycoproteins in an identified neuron of Aplysia.
1975,
Pubmed
Ambron,
Synthesis and axonal transport of membrane glycoproteins in an identified serotonergic neuron of Aplysia.
1980,
Pubmed
Currie,
Axonal transport of lipid in goldfish optic axons.
1978,
Pubmed
Edstrom,
Fast axonal transport in vitro in the sciatic system of the frog.
1972,
Pubmed
Edström,
Rapid axonal transport in vitro in the sciatic system of the frog of fucose-, glucosamine- and sulphate-containing material.
1972,
Pubmed
Edström,
Temperature effects on fast axonal transport of proteins in vitro in frog sciatic nerves.
1973,
Pubmed
Forman,
Rapid axonal transport of ( 3 H)fucosyl glycoproteins in the goldfish optic system.
1972,
Pubmed
Glazer,
The differential sensitivity of free and membrane-bound polyribosomes to inhibitors of protein synthesis.
1972,
Pubmed
Grafstein,
Axonal transport of phospholipid in goldfish optic system.
1975,
Pubmed
Grafstein,
Intracellular transport in neurons.
1980,
Pubmed
Gross,
A quantitative analysis of isotope concentration profiles and rapid transport velocities in the C-fibers of the garfish olfactory nerve.
1975,
Pubmed
Hammerschlag,
Initiation of fast axonal transport: involvement of calcium during transfer of proteins from Golgi apparatus to the transport system.
1979,
Pubmed
Hammond,
Inhibition of the rapid movement of optically detectable axonal particles colchicine and vinblastine.
1977,
Pubmed
,
Xenbase
Hines,
Release of protein from axons during rapid axonal transport: an in vitro preparation.
1977,
Pubmed
Longo,
Relation of somal lipid synthesis to the fast axonal transport of protein and lipid.
1980,
Pubmed
McEwen,
Fast and slow components in axonal transport of protein.
1968,
Pubmed
Neale,
Bidirectional axonal transport of 45Ca2+: studies in isolated frog sensory, motor and sympathetic neurons, Aplysia cerebral ganglion and the goldfish visual system.
1977,
Pubmed
Ochs,
Somal site of synthesis of fast transported materials in mammalian nerve fibers.
1969,
Pubmed
Ochs,
Retention and redistribution of proteins in mammalian nerve fibres by axoplasmic transport.
1975,
Pubmed
Schwartz,
Axonal transport: components, mechanisms, and specificity.
1979,
Pubmed
Sherbany,
Membrane glycolipids: regional synthesis and axonal transport in a single identified neuron of Aplysia californica.
1979,
Pubmed
Snyder,
Application of a multiwire proportional chamber to the detection of axoplasmic transport.
1976,
Pubmed
,
Xenbase
Snyder,
A multiwire proportional chamber study of axoplasmic transport in frog sciatic nerve involving interruption of somatic supply.
1979,
Pubmed
,
Xenbase
Snyder,
Rapid orthograde transport of 32P-labelled material in amphibian sensory axons: a multiwire proportional chamber study.
1980,
Pubmed
,
Xenbase
