Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Planta
2006 Aug 01;2243:569-81. doi: 10.1007/s00425-006-0246-8.
Show Gene links
Show Anatomy links
Expression of a peroxisome proliferator-activated receptor gene (xPPARalpha) from Xenopus laevis in tobacco (Nicotiana tabacum) plants.
Nila AG
,
Sandalio LM
,
López MG
,
Gómez M
,
del Rio LA
,
Gómez-Lim MA
.
???displayArticle.abstract???
In this work, we have genetically transformed tobacco (Nicotiana tabacum) plants with the peroxisome proliferator-activated receptor cDNA (xPPARalpha) from Xenopus laevis, which is a transcriptional factor involved in the peroxisomal proliferation and induction of fatty acid beta-oxidation in animal cells. Several transgenic lines were generated and one representative line (T) from the R2 generation was selected for further studies. Analysis of free fatty acids revealed that unsaturated fatty acids such as C16:2 and C16:3 were deficient in line T, whereas saturated fatty acids like C16:0, C18:0, and C20:0 were more abundant than in non-transformed plants. Acyl-CoA oxidase (ACOX) activity was assayed as a marker enzyme of beta-oxidation in crude leaf extracts and it was found that in line T there was a threefold increase in enzyme activity. We also found that the peroxisome population was increased and that catalase (CAT) activity was induced by clofibrate, a known activator of xPPARalpha protein, in leaves from line T. Taken together, these findings suggest that xPPARalpha is functional in plants and that its expression in tobacco leads to changes in general lipid metabolism and peroxisomal proliferation as reported in animal cells. Furthermore, it indicates that there is an endogenous ligand in tobacco cells able to activate xPPARalpha.
Aebi,
Catalase in vitro.
1984,
Pubmed
Arabidopsis Genome Initiative,
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
2000,
Pubmed
Blanquart,
Peroxisome proliferator-activated receptors: regulation of transcriptional activities and roles in inflammation.
2003,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Chapple,
MOLECULAR-GENETIC ANALYSIS OF PLANT CYTOCHROME P450-DEPENDENT MONOOXYGENASES.
1998,
Pubmed
Corpas,
Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells.
2001,
Pubmed
Desvergne,
Peroxisome proliferator-activated receptors: nuclear control of metabolism.
1999,
Pubmed
Dommes,
A convenient assay for acyl-CoA-dehydrogenases.
1976,
Pubmed
Dreyer,
Control of the peroxisomal beta-oxidation pathway by a novel family of nuclear hormone receptors.
1992,
Pubmed
,
Xenbase
Gerhardt,
Localization of β-oxidation enzymes in peroxisomes isolated from nonfatty plant tissues.
1983,
Pubmed
Graham,
Pathways of straight and branched chain fatty acid catabolism in higher plants.
2002,
Pubmed
Green,
PPAR: a mediator of peroxisome proliferator action.
1995,
Pubmed
Guo,
Identification of a plant nitric oxide synthase gene involved in hormonal signaling.
2003,
Pubmed
Gutiérrez-Ortega,
Expression of a single-chain human interleukin-12 gene in transgenic tobacco plants and functional studies.
2004,
Pubmed
Hayashi,
Entering a new era of research on plant peroxisomes.
2003,
Pubmed
Igamberdiev,
The role of peroxisomes in the integration of metabolism and evolutionary diversity of photosynthetic organisms.
2002,
Pubmed
Johnson,
Regulation of P450 4A expression by peroxisome proliferator activated receptors.
2002,
Pubmed
Keller,
Fatty acids and retinoids control lipid metabolism through activation of peroxisome proliferator-activated receptor-retinoid X receptor heterodimers.
1993,
Pubmed
Li,
Conservation of function between mammalian and plant steroid 5alpha-reductases.
1997,
Pubmed
Marcus,
Diverse peroxisome proliferator-activated receptors bind to the peroxisome proliferator-responsive elements of the rat hydratase/dehydrogenase and fatty acyl-CoA oxidase genes but differentially induce expression.
1993,
Pubmed
,
Xenbase
Mehendale,
PPAR-alpha: a key to the mechanism of hepatoprotection by clofibrate.
2000,
Pubmed
Millar,
Accumulation of very-long-chain fatty acids in membrane glycerolipids is associated with dramatic alterations in plant morphology.
1998,
Pubmed
Na,
Peroxisome proliferator-activated receptor gamma (PPARgamma) ligands as bifunctional regulators of cell proliferation.
2003,
Pubmed
Palma,
Peroxisome proliferation and oxidative stress mediated by activated oxygen species in plant peroxisomes.
1991,
Pubmed
Pastori,
Natural Senescence of Pea Leaves (An Activated Oxygen-Mediated Function for Peroxisomes).
1997,
Pubmed
Pinot,
[omega]-Hydroxylation of Oleic Acid in Vicia sativa Microsomes (Inhibition by Substrate Analogs and Inactivation by Terminal Acetylenes).
1993,
Pubmed
Polashock,
Expression of the Yeast Delta-9 Fatty Acid Desaturase in Nicotiana tabacum.
1992,
Pubmed
Qi,
Peroxisome proliferator-activated receptors, coactivators, and downstream targets.
2000,
Pubmed
Ruyter,
Peroxisome proliferator activated receptors in Atlantic salmon (Salmo salar): effects on PPAR transcription and acyl-CoA oxidase activity in hepatocytes by peroxisome proliferators and fatty acids.
1997,
Pubmed
Sayanova,
Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6-desaturated fatty acids in transgenic tobacco.
1997,
Pubmed
Schardl,
Design and construction of a versatile system for the expression of foreign genes in plants.
1987,
Pubmed
Shay,
Regulation of gene transcription by botanicals: novel regulatory mechanisms.
2005,
Pubmed
Takahashi,
Dual action of isoprenols from herbal medicines on both PPARgamma and PPARalpha in 3T3-L1 adipocytes and HepG2 hepatocytes.
2002,
Pubmed
Tan,
Multiple expression control mechanisms of peroxisome proliferator-activated receptors and their target genes.
2005,
Pubmed
Tijet,
Functional expression in yeast and characterization of a clofibrate-inducible plant cytochrome P-450 (CYP94A1) involved in cutin monomers synthesis.
1998,
Pubmed
Torra,
Peroxisome proliferator-activated receptors: from transcriptional control to clinical practice.
2001,
Pubmed
Tsydendambaev,
Identification of unusual fatty acids of four alpine plant species from the Pamirs.
2004,
Pubmed
Vert,
Molecular mechanisms of steroid hormone signaling in plants.
2005,
Pubmed
Weber,
Fatty acid-derived signals in plants.
2002,
Pubmed
del Rio LA,
The activated oxygen role of peroxisomes in senescence.
1998,
Pubmed
del Río,
Nitric oxide and nitric oxide synthase activity in plants.
2004,
Pubmed
del Río,
Reactive oxygen species, antioxidant systems and nitric oxide in peroxisomes.
2002,
Pubmed
